|本期目录/Table of Contents|

[1]吴美儒,周毅峰,唐巧玉.1株耐硒凝结芽孢杆菌的分离鉴定及其培养条件的优化[J].江苏农业科学,2019,47(04):256-259.
　Wu Meiru,et al.Isolation and identification of a selenium-tolerant Bacillus coagulans strain and optimization of its culture conditions[J].Jiangsu Agricultural Sciences,2019,47(04):256-259.
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1株耐硒凝结芽孢杆菌的分离鉴定
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第04期
页码:
256-259
栏目:
资源与环境
出版日期:
2019-03-15

文章信息/Info

Title:
Isolation and identification of a selenium-tolerant Bacillus coagulans strain and optimization of its culture conditions
作者:
吴美儒1;  周毅峰2;  唐巧玉1
1.湖北民族学院生物资源保护与利用湖北省重点实验室，湖北恩施 445000； 2.湖北民族学院生物科学与技术学院，湖北恩施 445000
Author(s):
Wu Meiru; et al
关键词:
硒; 凝结芽孢杆菌; 分离; 培养条件; 生长曲线
Keywords:
-
分类号:
S182
DOI:
-
文献标志码:
A
摘要:
用含硒培养基，采用平板稀释分离法，从湖北恩施渔塘坝高硒环境中分离鉴定1株凝结芽孢杆菌。为确定该凝结芽孢杆菌培养基及培养条件，采用单因素和正交试验法对凝结芽孢杆菌的摇瓶发酵培养条件(碳源、氮源、温度和转速)进行了优化，为确定其培养时间作出了生长曲线。结果表明，温度对凝结芽孢杆菌生长的影响最大，其后依次为氮源、碳源和转速；最适培养基为：葡萄糖11 g/L，牛肉浸膏11 g/L，氯化钠5 g/L；最适培养条件为：温度35 ℃，转速160 r/min；(2)从生长曲线可看出，0~1.5 h为停滞期，1.5~10 h为对数生长期，10~20 h为稳定期，最佳培养时间为10 h。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2017-10-24
基金项目：国家自然科学基金(编号：31360498)；湖北省教育厅重点项目(编号：D20131902)。
作者简介：吴美儒(1993—)，女，湖北恩施人，硕士研究生，主要从事植物硒代谢的研究：E-mail：1060198820@qq.com。
通信作者：唐巧玉，硕士，副教授，主要从事微量元素硒的研究。E-mail：2004039@hbmy.edu.cn。
更新日期/Last Update: 2019-02-20