|本期目录/Table of Contents|

[1]徐劼,段德超.镉胁迫对芹菜生理特性及细胞超微结构的影响[J].江苏农业科学,2019,47(05):95-99.
 Xu Jie,et al.Effects of cadmium stress on physiological property and cell ultrastructure of celery[J].Jiangsu Agricultural Sciences,2019,47(05):95-99.
点击复制

镉胁迫对芹菜生理特性及细胞超微结构的影响(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第05期
页码:
95-99
栏目:
园艺与林学
出版日期:
2019-03-30

文章信息/Info

Title:
Effects of cadmium stress on physiological property and cell ultrastructure of celery
作者:
徐劼1 段德超2
1.嘉兴学院生物与化学工程学院,浙江嘉兴 314001; 2.浙江博世华环保科技有限公司,浙江杭州 310015
Author(s):
Xu Jieet al
关键词:
芹菜富集系数超微结构生理特性转运能力细胞
Keywords:
-
分类号:
X53;S636.301
DOI:
-
文献标志码:
A
摘要:
采用水培试验,研究不同浓度镉(Cd)胁迫下Cd在芹菜不同器官组织中的积累及对芹菜生理生化指标、细胞超微结构的影响。结果表明,Cd浓度为40 mg/L时,芹菜叶、茎、根中的Cd含量相对最高,分别为16.36、65.78、213.06 mg/L;Cd浓度为10 mg/L时,芹菜对Cd的富集和转运能力相对最强,富集系数、转运系数分别为5.83、0.62,同时,叶绿素a、叶绿素b、总叶绿素含量处于最高水平;Cd胁迫导致芹菜叶片细胞壁内侧模糊,轮廓不饱满,淀粉颗粒增大、变多,叶绿体出现肿胀,且基粒类囊体呈波浪状,并处于解离状态,而根尖细胞的细胞基质变稀,液泡数量明显增多,细胞核仁消失,染色质外溢,线粒体出现肿胀;Cd胁迫浓度分别为5、10、20、40 mg/L时,芹菜叶组织中的可溶性蛋白含量分别为清水处理(CK)的98.73%、96.55%、91.28%、86.16%,而根中的蛋白含量分别为对照的99.74%、109.98%、132.76%、157.18%;随Cd胁迫浓度的增大,芹菜的根系活力逐渐降低,与CK相比分别显著下降15.33%、57.72%、58.69%、70.59%(P<0.05)。
Abstract:
-

参考文献/References:

[1]Jin Y H,Clark A B,Slebos R J,et al. Cadmium is a mutagen that acts by inhibiting mismatch repair[J]. Nature Genetics,2003,34(3):326-329.
[2]Basa B,Lattanzio G,Solti A,et al. Changes induced by cadmium stress and iron deficiency in the composition and organization of thylakoid complexes in sugar beet (Beta vulgaris L.)[J]. Environmental and Experimental Botany,2014,101(5):1-11.
[3]肖旭峰,解庆娓,范淑英,等. 镉、铅胁迫对芹菜生长及抗氧化酶活性的影响[J]. 江西农业大学学报,2015,37(5):793-797.
[4]徐劼,保积庆. 芹菜根细胞壁对镉的吸附固定机制及其FTIR表征研究[J]. 环境科学学报,2015,35(8):2605-2612.
[5]Du R J,He E K,Tang Y T,et al. How phytohormone IAA and chelator EDTA affect lead uptake by Zn/Cd hyperaccumulator Picris divaricate[J]. International Journal of Phytoremediation,2011,13(10):1024-1036.
[6]张静,赵秀侠,汪翔,等. 重金属镉(Cd)胁迫对水芹生长及生理特性的影响[J]. 植物生理学报,2015,51(11):1969-1974.
[7]王国栋,陈云,梁飞,等. 控墒补灌对新疆春玉米产量和穗叶光合特性的影响[J]. 应用生态学报,2016,27(8):2499-2506.
[8]徐臣善. 钙对盐胁迫下小金海棠幼苗生物量及抗氧化系统的影响[J]. 植物生理学报,2014,50(6):817-822.
[9]程海宽,张彪,景鑫鑫,等. 玉米对铅胁迫的响应及体内铅化学形态研究[J]. 环境科学,2015,36(4):1468-1473.
[10]Yamauchi T,Watanabe K,Fukazawa A,et al. Ethylene and reactive oxygen species are involved in root aerenchyma formation and adaptation of wheat seedlings to oxygen-deficient conditions[J]. Journal of Exprimental Botany,2014,65(1):261-273.
[11]徐劼. 茶树(Camellia sinensis L.)对铅的吸收累积及耐性机制研究[D]. 杭州:浙江大学,2011.
[12]Xu C,Chen X,Duan D,et al. Effect of heavy-metal-resistant bacteria on enhanced metal uptake and translocation of the Cu-tolerant plant,Elsholtzia splendens[J]. Environmental Science and Pollution Research,2015,22(7):5070-5081.
[13]邹金华,张忠贵,魏爱琪. 毛葱的镉吸收积累及亚细胞分布特征[J]. 天津师范大学学报(自然科学版),2014,34(1):72-77.
[14]Yin A G,Yang Z Y,Ebbs S,et al. Effects of phosphorus on chemical forms of Cd in plants of four spinach (Spinacia oleracea L.) cultivars differing in Cd accumulation[J]. Environmental Science and Pollution Research,2016,23(6):5753-5762.
[15]Wang A Y,Wang M Y,Liao Q,et al. Characterization of Cd translocation and accumulation in 19 maize cultivars grown on Cd-contaminated soil:implication of maize cultivar selection for minimal risk to human health and for phytoremediation[J]. Environmental Science and Pollution Research,2016,23(6):5410-5419.
[16]Huang B F,Xin J L,Dai H W,et al. Root morphological responses of three hot pepper cultivars to Cd exposure and their correlations with Cd accumulation[J]. Environmental Science and Pollution Research,2015,22(2):1151-1159.
[17]刘卫国,丁俊祥,邹杰,等. NaCl对齿肋赤藓叶肉细胞超微结构的影响[J]. 生态学报,2016,36(12):3556-3563.
[18]Nash W W,Poor B W,Jenkins K D. The uptake and subcellular-distribution of lead in developing sea-urchin embryos[J]. Comparative Biochemistry and Physiology Part C:Comparative Pharmacology,1981,69(2):205-211.
[19]Brain G S,Martin S W. Ultrastructure and the biology of plant cells[M]. New York:Edward Arnold,1975.
[20]Yao Y C,Wang S H,Kong Y. Characteristics of photosynthesis mechanism in different peach species under low light intensity[J]. Scientia Agricultura Sinica,2007,40(4):855-863.
[21]Asada K. The water-water cycle in chloroplasts:scavenging of active oxygens and dissipation of excess photons[J]. Annual Review of Plant Biology,1999,50:601-639.
[22]Jiang M Y,Yang W Y,Xu J. Active oxygen damage effect of chlorophyll degradation in rice seedlings under osmotic stress[J]. Acta Botanica Sinica,1994,36(4):289-295.
[23]Hall J L. Electron microscopy and cytochemistry of plant cells[M]. New York:North-holland Biomedical Press,1978.
[24]段昌群,王焕校. 重金属对蚕豆的细胞遗传学毒理作用和对蚕豆根尖微核技术的探讨[J]. 植物学报,1995,37(1):14-24.
[25]侯江涛,高启明,克热木·伊力. NaCl胁迫对两种扁桃幼苗根系K+、Na+、Cl-含量和超微结构的影响[J]. 北方园艺,2014(23):1-5.
[26]宇克莉,孟庆敏,邹金华. 镉对玉米幼苗生长、叶绿素含量及细胞超微结构的影响[J]. 华北农学报,2010,25(3):118-123.
[27]李铮铮,伍钧,唐亚,等. 铅、锌及其交互作用对鱼腥草(Houttuynia cordata)叶绿素含量及抗氧化酶系统的影响[J]. 生态学报,2007,27(12):5441-5446.
[28]Bhaduri A M,Fulekar M H. Antioxidant enzyme responses of plants to heavy metal stress[J]. Reviews in Environmental Science and Biotechnology,2012,11(1):55-69.
[29]彭玲,贾芬,田小平,等. 硒对油菜根尖镉胁迫的缓解作用[J]. 环境科学学报,2015,35(8):2597-2604.
[30]董如茵,徐应明,王林,等. 土施和喷施锌肥对镉低积累油菜吸收镉的影响[J]. 环境科学学报,2015,35(8):2589-2596.
[31]郭艳丽,台培东,韩艳萍,等. 镉胁迫对向日葵幼苗生长和生理特性的影响[J]. 环境工程学报,2009,3(12):2291-2296.
[32]李佩华,刘小文. 重金属铅、镉胁迫对马铃薯生长及抗氧化酶系统的影响[J]. 云南农业大学学报(自然科学版),2014,29(5):746-751.
[33]吴桂容,严重玲. 镉对桐花树幼苗生长及渗透调节的影响[J]. 生态环境,2006,15(5):1003-1008.
[34]Bartolf M,Brennan E,Price C A. Partial characterization of a cadmium-binding protein from the roots of cadmium-treated tomato[J]. Plant Physiology,1980,66(3):438-441.
[35]Wang X E,Shi G X,Xu Q S,et al. Exogenous polyamnines enhance copper tolerance of Nymphoides peltatum[J]. Journal of Plant Physiology,2007,164(8):1062-1070.
[36]王玉凤,王庆祥,商丽威. 钙对NaCl胁迫下玉米幼苗根系活力和有机渗透调节物质含量的影响[J]. 玉米科学,2008,16(2):66-70.

相似文献/References:

[1]史景允,于伟红,梁秋生.蓖麻对镉污染土壤的修复潜力[J].江苏农业科学,2014,42(11):386.
 Shi Jingyun,et al(8).Potential repairing of cadmium contaminated soil by castor oil plant[J].Jiangsu Agricultural Sciences,2014,42(05):386.
[2]房春生,王帆,刘多,等.模拟酸雨对白菜体内铅、镉富集的影响[J].江苏农业科学,2013,41(08):323.
 Fang Chunsheng,et al.Effects of simulated acid rain on lead and cadmium accumulation in Brassica chinensis ssp. chinensis[J].Jiangsu Agricultural Sciences,2013,41(05):323.
[3]雷忻,郭兆权,延志莲,等.镉胁迫对泥鳅血清卵黄蛋白原的诱导作用[J].江苏农业科学,2013,41(09):321.
 Lei Xin,et al.Inductive effect of cadmium stress on serum vitellogenin in Misgurnus anguillicaudatus[J].Jiangsu Agricultural Sciences,2013,41(05):321.
[4]郭继斌,王莉,韩娇,等.联合浸提法测定土壤有效态镉[J].江苏农业科学,2016,44(03):369.
 Guo Jibin,et al.Determination of soil available Cd content by universal extraction method[J].Jiangsu Agricultural Sciences,2016,44(05):369.
[5]高华健,王玉祯,侯丹,等.锌调节镉胁迫水稻幼苗根系生长的生理机制[J].江苏农业科学,2013,41(12):48.
 Gao Huajian,et al.Physiological effect of zinc on growth of rice seedling roots under cadmium stress[J].Jiangsu Agricultural Sciences,2013,41(05):48.
[6]姚茹,黎小正.广西沿海主要贝类养殖区海水、表层沉积物及近江牡蛎体内重金属镉监测与评价[J].江苏农业科学,2014,42(01):316.
 Yao Ru,et al.Monitoring and comprehensive assessment of Cd in sea water,surface sediments and body of Crassostrea rivularis in main shellfish culture areas of Guangxi coastal waters[J].Jiangsu Agricultural Sciences,2014,42(05):316.
[7]刘标,尹红梅,陈薇,等.高效镉吸附菌株的筛选及生物学特性[J].江苏农业科学,2014,42(03):316.
 Liu Biao,et al.Screening and biological characteristics of strains with strong cadmium adsorption ability[J].Jiangsu Agricultural Sciences,2014,42(05):316.
[8]刘思思,高旋旋,胡竹青,等.镉诱导锦鲤肾脏氧化性DNA损伤及谷胱甘肽抗氧化系统改变[J].江苏农业科学,2016,44(02):272.
 Liu Sisi,et al.Cadmium induced renal oxidative DNA damage and glutathione antioxidant system change of brocade carp[J].Jiangsu Agricultural Sciences,2016,44(05):272.
[9]宋晓慧,陆引罡,何丹,等.烟草对镉的吸收及镉在亚细胞中的分布[J].江苏农业科学,2014,42(05):116.
 Song Xiaohui,et al.Absorbtion and subcellular distribution of cadmium in tobacco[J].Jiangsu Agricultural Sciences,2014,42(05):116.
[10]张美德,艾伦强,卢超,等.硒对镉胁迫下白术幼苗生理特性的影响[J].江苏农业科学,2015,43(10):306.
 Zhang Meide,et al.Effect of selenium on physiological characteristics of Atractylodes macrocephala Koidz. seedlings under cadmium stress[J].Jiangsu Agricultural Sciences,2015,43(05):306.

备注/Memo

备注/Memo:
收稿日期:2017-12-12
基金项目:浙江省嘉兴市科技计划(编号:2017BY18065)。
作者简介:徐劼(1975—),男,辽宁辽阳人,博士,讲师,从事土壤重金属植物污染化学与农产品安全研究。Tel:(0573)83643295;E-mail:xujie1688@126.com。
更新日期/Last Update: 2019-03-05