|本期目录/Table of Contents|

[1]齐素敏,陈丹丹,李圆圆,等.玫烟色棒束孢NBL-Z8的培养及对桃蚜的侵染力[J].江苏农业科学,2021,49(10):82-86.
 Qi Sumin,et al.Cultivation of Isaria fumosorosea NBL-Z8 and its infectivity to Myzus persicae[J].Jiangsu Agricultural Sciences,2021,49(10):82-86.
点击复制

玫烟色棒束孢NBL-Z8的培养及对桃蚜的侵染力(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第10期
页码:
82-86
栏目:
植物保护
出版日期:
2021-05-20

文章信息/Info

Title:
Cultivation of Isaria fumosorosea NBL-Z8 and its infectivity to Myzus persicae
作者:
齐素敏 陈丹丹 李圆圆 冉新炎 韩广泉 陶宁 王丽荣
山东碧蓝生物科技有限公司/泰安市植物微生态制剂重点实验室,山东泰安 271000
Author(s):
Qi Suminet al
关键词:
玫烟色棒束孢液体培养桃蚜侵染力测定生防潜力
Keywords:
-
分类号:
S433.39;S182
DOI:
-
文献标志码:
A
摘要:
通过探索玫烟色棒束孢NBL-Z8的最佳培养条件并测定该菌对桃蚜[Myzus persicae (Sulzer)]的侵染力,以期为筛选出对桃蚜具有高毒力的微生物杀虫剂提供依据。采用单因素分析法测定菌株的最佳培养条件,并采用喷雾法测定菌株NBL-Z8对桃蚜的致病力。结果显示,NBL-Z8菌株的最佳碳源为蔗糖,最佳氮源为蛋白胨,最佳培养条件为温度25 ℃、初始pH值7、转速180 r/min、接种量6%;菌株的室内致病力测定结果表明,当孢子浓度为1×108 CFU/mL 时,对桃蚜的致死中时间(LT50)为3.118 d,接菌7 d时致死中浓度(LC50)为1.367×104 CFU/mL。由研究结果可以看出,玫烟色棒束孢NBL-Z8对桃蚜具有较强的致病力,可作为桃蚜生防制剂开发中的潜力菌株。
Abstract:
-

参考文献/References:

[1]von Dohlen C D,Rowe C A,Heie O E,et al. A test of morphological hypotheses for tribal and subtribal relationships of Aphidinae (Insecta:Hemiptera:Aphididae) using DNA sequences[J]. Molecular Phylogenetics and Evolution,2006,38(2):316-329.
[2]郑竹胜,邢鲲,赵飞,等. 甘蓝蚜虫田间防治研究[J]. 中国果菜,2015,35(4):59-61.
[3]闰彦萍,王海荣,韩巨才. 山西甘蓝蚜对7种杀虫剂的抗性监测[J]. 安徽农业科学,2006,34(18):4684-4685.
[4]Nauen R,Elbert A. European monitoring of resistance to insecticides in Myzus persicae and Aphis gossypii (Hemiptera:Aphididae) with special reference to imidacloprid[J]. Bulletin of Entomological Research,2003,93(1):47.
[5]武银玉,曹亚萍,杨秀丽,等. 麦蚜抗药性现状及抗性治理研究进展[J]. 小麦研究,2017,38(2):1-8.
[6]苗艳香. 蔬菜蚜虫抗药性现状及治理策略[J]. 热带农业工程,2019,43(1):68-70.
[7]Srigiriraju L,Semtner P J,Bloomquist J R. Monitoring for imidacloprid resistance in the tobacco-adapted form of the green peach aphid,Myzus persicae (Sulzer) (Hemiptera:Aphididae) in the eastern United States[J]. Pest Management Science,2010,66(6):676-685.
[8]Zimmermann G. The entomopathogenic fungi Isaria farinosa (formerly Paecilomyces farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus):biology,ecology and use in biological control[J]. Biocontrol Science and Technology,2008,18(9):865-901.
[9]曹娜. 玫烟色棒束孢发酵条件及对刺吸式口器害虫控制潜力研究[D]. 合肥:安徽农业大学,2018.
[10]朱丽梅,严慧,方慧子,等. 玫烟色棒束孢QH4对蚜虫和烟粉虱杀虫活性的研究[J]. 金陵科技学院学报,2018,34(1):84-87.
[11]邓建华,吴兴富,庄辉,等. 两种拟青霉对烟蚜感染作用试验[J]. 烟草科技,2005(3):46-48.
[12]孙莉,张艳璇,赵玲玲,等. 利用胡瓜新小绥螨携带玫烟色拟青霉菌兼防茄子蚜虫和叶螨[J]. 中国农学通报,2015,31(20):91-96.
[13]邢培翔,刁红亮,韩志慧,等. 烟色棒束孢IF-1106对2种果树蚜虫的致病力[J]. 山西农业科学,2019,47(11):2029-2033,2045.
[14]李农昌,樊美珍,李春如,等. 白僵菌有关培养条件及其与毒力关系的研究[J]. 安徽农业大学学报,1996,23(3):254-259.
[15]张仙红,张奂,张未仲. 玫烟色拟青霉最适液体培养条件的研究[J]. 微生物学杂志,2006,26(6):15-18.
[16]田晶. 玫烟色棒束孢生物学特性及对烟粉虱致病作用研究[D]. 晋中:山西农业大学,2014.
[17]Meyling N V,Eilenberg J. Ecology of the entomopathogenic fungi Beanveria bassiana and Metarhnm anisopliae in temperate agroecosystems:potential for conservation biological control[J]. Biological Control,2007,43(2):145-155.
[18]Shan L T,Feng M G. Evaluation of the biocontrol potential of various Metarhiziu misolates against green peach aphid Myzus persicac (Homoptcra:Aphididae)[J]. Pest Management Scicnce,2010,66(6):669-675.
[19]Poprawski T J,Parker P E,Tsai J H,et al. Laboratory and field evalua tion of hyphomycete insect pathogenic fungi for control of brown citrus aphid (Homoptera:Aphididae)[J]. Environmental Entomology,1999,28(2):315-321.
[20]Diaz B M,Oggcrin M,Lastra C C L,et al. Characterization and virulence of Lecanicillium lecanii against diffetent aphid species[J]. BioControl,2009,54(6):825-835.
[21]刘浩,张龙,张宗山. 蜡蚧轮枝菌对枸杞蚜虫的室内毒力和常用药剂敏感性测定[J]. 中国蔬菜,2012(4):87-90.
[22]Grenier A M,Duport G,Pages S,et al . The phytopathogen Diekeya dadantii (Frwinia chrysanthcmi 3937) is a pathogen of the pea aphid[J]. Applied and Environmental Microbiology,2006,72(3):1956-1965.
[23]Costeeharcyre D,Balmand S,Condemine G,et al. Dickeya dadantii,aplant pathogenic bacterium producing Cyt-like entomotoxins,causes septicemia in the pea aphid Acyrthosiphon pisum[J]. PLoS One,2012,7(1):e30702.
[24]陈巍巍,冯光明. 四株玫烟色拟青霉作为桃蚜微生物防治因子的潜力评价[J]. 浙江大学学报(农业与生命科学版),1999,25(6):563-568.
[25]张仙红,嵇能焕. 玫烟色拟青霉菌株的毒力测定[J]. 山西农业大学学报,2006,26(1):24-26.
[26]孟豪,田晶,付淑慧,等. 玫烟色棒束孢与球孢白僵菌对桃蚜致病力对比[J]. 植物保护学报,2014,41(6):717-722.
[27]周鹏飞,张婧,张博文,等. 玫烟色棒束孢的遗传地理差异及对B型烟粉虱的致病性[J]. 华南农业大学学报,2018,39(3):60-64.
[28]Flores-Macias A,Diaz M P,Ramos-Lopez M A,et al. Study of the entomopathogenic fungus Isaria fumosorosea as microbiological control of the white fly Bemicia tabaci[J]. Interciencia,2013,38(7):523-527.
[29]Tian J,Diao H L,Li L,Hao C,et al. Pathogenicity of Isaria fumosorosea to Bemisia tabaci,with some observations on the fungal infection process and host immune response[J]. Journal of Invertebrate Pathology,2015,130:147-153.
[30]Hoy M,Singh R. Evaluations of a novel isolate of Isaria fumosorosea for control of the Asian citrus psyllid,Diaphorina citri (Hemiptera:Psyllidae)[J]. Florida Entomologist,2010,93:24-32.
[31]代晓彦,任素丽,周雅婷,等. 黄龙病媒介昆虫柑橘木虱生物防治新进展[J]. 中国生物防治学报,2014,30(3):414-419.
[32]吕利华,何余容,武亚敬,等. 玫烟色拟青霉对小菜蛾致病力的时间-剂量-死亡率模型模拟[J]. 昆虫学报,2007,50(6):567-573.
[33]Shi W B,Feng M G. Ovicidal activity of two fungal pathogens (Hyphomycetes) against Tetranychus cinnabarinus (Acarina:Tetranichidae)[J]. Chinese Science Bulletin,2004,49(3):263-267.
[34]de Faria M R,Wraight S P. Mycoinsecticides and Mycoacaricides:a comprehensive list with worldwide coverage and international classification of formulation types[J]. Biological Control,2007,43(3):237-256.

相似文献/References:

[1]张梅秀,魏玉杰,臧广鹏,等.脱毒啤酒花的高温快繁技术[J].江苏农业科学,2013,41(05):53.
 Zhang Meixiu,et al.Study on high temperature rapid propagation techniques of virus-free Humulus lupulusL.[J].Jiangsu Agricultural Sciences,2013,41(10):53.
[2]钟万芳,瞿钰峰,郭慧芳.高效防治烟粉虱的玫烟色棒束孢JZ-7的特性[J].江苏农业科学,2013,41(07):104.
 Zhong Wanfang,et al.Characteristics of Isaria fumosorosea JZ-7 with high toxicity to Bemisia tabaci[J].Jiangsu Agricultural Sciences,2013,41(10):104.
[3]方中明,于恒,白根祥,等.香水百合组织培养和快速繁殖条件的优化[J].江苏农业科学,2016,44(08):81.
 Fang Zhongming,et al.Optimization of tissue culture and rapid propagation conditions of perfume lily[J].Jiangsu Agricultural Sciences,2016,44(10):81.
[4]史俊,席刚俊,贾君,等.不同因素对白芨原球茎和假鳞茎形成的影响[J].江苏农业科学,2018,46(18):153.
 Shi Jun,et al.Effects of different factors on formation of protocorm and pseudobulb of Bletilla striata[J].Jiangsu Agricultural Sciences,2018,46(10):153.
[5]张杰,许风国.高产和高生物活性的铁皮石斛液体培养体系研究[J].江苏农业科学,2019,47(06):43.
 Zhang Jie,et al.Study on liquid culture system of Dendrobium candidum with high yield and high biological activity[J].Jiangsu Agricultural Sciences,2019,47(10):43.

备注/Memo

备注/Memo:
收稿日期:2020-08-20
基金项目:山东省重点研发计划(编号:2018JHZ012);泰山产业领军人才项目(编号:鲁政办字[2018]246号);2017年“外专双百计划”项目(编号:鲁政办字[2017]144号)。
作者简介:齐素敏(1989—),女,山东淄博人,硕士,助理研究员,主要从事植物微生态制剂的研发工作。E-mail:qsm20095089@126.com。
更新日期/Last Update: 2021-05-20