|本期目录/Table of Contents|

[1]汪园,熊雨舟,王世岩,等.不同施硒方法对豇豆豆荚中硒形态与含量的影响[J].江苏农业科学,2022,50(22):145-152.
 Wang Yuan,et al.Effects of different selenium application methods on form and content of selenium in cowpea pods[J].Jiangsu Agricultural Sciences,2022,50(22):145-152.
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不同施硒方法对豇豆豆荚中硒形态与含量的影响(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第22期
页码:
145-152
栏目:
园艺与林学
出版日期:
2022-11-20

文章信息/Info

Title:
Effects of different selenium application methods on form and content of selenium in cowpea pods
作者:
汪园123熊雨舟123王世岩123饶申13程华13熊银13程水源123李丽134
1.武汉轻工大学/国家富硒农产品加工技术与研发专业中心,湖北武汉 430023; 2.武汉轻工大学生命科学与技术学院,湖北武汉 430023;3.武汉轻工大学硒科学与工程现代产业学院,湖北武汉 430023; 4.粮食作物种质创新与遗传改良湖北省重点实验室,湖北武汉 430023
Author(s):
Wang Yuanet al
关键词:
长豇豆品种纳米硒亚硒酸钠施用浓度
Keywords:
-
分类号:
S643.406
DOI:
-
文献标志码:
A
摘要:
为了确定改善豇豆硒营养的硒肥调控措施,以矮蔓豇豆美国无架豆、有架豇豆之豇28/2为试验材料,以亚硒酸钠(Na2SeO3)、纳米硒(SeNPs)为硒源,在2个不同的生长季节分别开展硒强化栽培的定点试验。在第1季,将外源硒浓度设置为0、0.1、0.5、1.0、2.5、3.0 mmol/L,研究不同硒浓度、不同硒源对豇豆豆荚中硒形态、硒含量的影响;在第2季,将外源硒浓度设置为0、0.5、2.5 mmol/L,比较不同施肥次数、不同采摘时间对豇豆豆荚中总硒含量的影响。结果表明,2个豇豆品种豆荚的硒含量均随施入的外源硒浓度的增加而增加,在相同浓度下,Na2SeO3对豆荚硒含量的提升效果更为明显。用不同外源硒处理后,豇豆豆荚中硒的主要存在形态为MeSeCys、SeMet,在有架豇豆豆荚中,这2种形态硒含量占总硒含量的比例分别为15.20%~36.53%、63.47%~74.64%,在矮蔓豇豆豆荚中,这2种形态硒含量的占比分别为14.50%~32.31%、55.00%~100.00%;无机硒形态均只能在高浓度处理组中被检测到。用2种硒源的硒处理后,低浓度硒处理组的矮蔓豇豆第2茬采摘的豆荚中总硒含量与第1茬相比略有降低,而高浓度处理组中与此相反,其中 2.5 mmol/L 纳米硒处理组中第2茬采摘的豆荚中硒含量显著升高;有架豇豆的表现与矮蔓豇豆不同,2.5 mmol/L纳米硒处理组中第2茬采摘的豆荚中硒含量显著下降。在2种硒源处理下,与初花期施1次硒相比,结荚期追施1次硒对豆荚中硒含量有一定的提升效果,但在高浓度亚硒酸钠处理组中,2次施肥会造成豆荚中硒含量的下降。由结果可知,叶片喷施纳米硒、亚硒酸钠处理能够有效提高豇豆豆荚的总硒含量,且豆荚中的硒形态以SeMet、MeSeCys为主,通过豇豆叶片吸收的硒优先转化为有机硒;不同外源硒种类、施用浓度、植物基因型对豇豆豆荚累积的硒形态、含量有明显影响;用2.5 mmol/L SeNPs在豇豆初花期进行叶面喷施(如有必要可在结荚期追施1次)是最佳处理。
Abstract:
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参考文献/References:

[1]程水源. 硒学导论[M]. 北京:中国农业出版社,2019:81-115.
[2]Wang J,Li H R,Yang L S,et al. Distribution and translocation of selenium from soil to highland barley in the Tibetan Plateau Kashin-Beck disease area[J]. Environmental Geochemistry and Health,2017,39(1):221-229.
[3]Li S M,Bauelos G S,Wu L H,et al. The changing selenium nutritional status of Chinese residents[J]. Nutrients,2014,6(3):1103-1114.
[4]Dinh Q T,Cui Z W,Huang J,et al. Selenium distribution in the Chinese environment and its relationship with human health:a review[J]. Environment International,2018,112:294-309.
[5]DAmato R,Regni L,Falcinelli B,et al. Current knowledge on selenium biofortification to improve the nutraceutical profile of food:a comprehensive review[J]. Journal of Agricultural and Food Chemistry,2020,68(14):4075-4097.
[6]Cai R,Hettiarachchy N S,Jalaluddin M. High-performance liquid chromatography determination of phenolic constituents in 17 varieties of cowpea[J]. Journal of Agricultural and Food Chemistry,2003,51(6):1623-1627.
[7]程安玮,吴剑夫,秦宏伟,等. 4种豆类中多酚、类黄酮含量及抗氧化活性研究[J]. 中国粮油学报,2017,32(10):28-32.
[8]汪宝根,吴新义,李素娟,等. 浙江省地方豇豆种质资源的鉴定与评价[J]. 植物遗传资源学报,2021,22(2):380-389.
[9]Silva V M,Boleta E H M,Martins J T,et al. Agronomic biofortification of cowpea with selenium:effects of selenate and selenite applications on selenium and phytate concentrations in seeds[J]. Journal of the Science of Food and Agriculture,2019,99(13):5969-5983.
[10]Block E,Birringer M,Jiang W Q,et al. Allium chemistry:synthesis,natural occurrence,biological activity,and chemistry of Se-alk(en)ylselenocysteines and their γ-glutamyl derivatives and oxidation products[J]. Journal of Agricultural and Food Chemistry,2001,49(1):458-470.
[11]张伟,万宣伍,田卉,等. 2种植物生长调节剂对豇豆产量和品质的影响[J]. 食品安全质量检测学报,2021,12(7):2624-2628.
[12]孙信成,张忠武,杨连勇,等. 密度与追肥量对豇豆产量及农艺性状的影响[J]. 中国农机化学报,2018,39(6):71-76.
[13]Rao S,Yu T,Cong X,et al. Integration analysis of PacBio SMRT-and Illumina RNA-seq reveals candidate genes and pathway involved in selenium metabolism in hyperaccumulator Cardanine violifolia[J]. BMC Plant Biology,2020,20(1):492-511.
[14]WHO. Vitamin and mineral requirements in human nutrition[M]. 2nd ed. Geneva:World Health Organization and Food and Agriculture Organization of the United Nations,2004.
[15]张志轩,高艳丽,汪妮,等. 豇豆新品种“全王绿宝特”的选育与特征特性[J]. 北方园艺,2018(13):209-210,2.
[16]吴正景,郭大龙,高文. 不同时期喷施亚硒酸钠对豌豆芽苗生长及硒含量的影响[J]. 种子,2008,27(8):40-42.
[17]刘芳,周乾坤,周守标,等. 施硒对紫云英生长、生理和硒积累特性的影响[J]. 土壤通报,2016,47(1):129-136.
[18]Zoltán K,ron S,Béla K,et al. Uptake dynamics of ionic and elemental selenium forms and their metabolism in multiple-harvested alfalfa (Medicago sativa L.)[J]. Plants,2021,10(7):1277.
[19]吴正景,王少先,蒋燕,等. 不同质量浓度亚硒酸钠对豌豆芽苗菜生长及硒含量的影响[J]. 广东微量元素科学,2004,11(12):35-38.
[20]Hu T,Li H F,Li J X,et al. Absorption and bio-transformation of selenium nanoparticles by wheat seedlings (Triticum aestivum L.)[J]. Frontiers in Plant Science,2018,9:597.
[21]Wang K,Wang Y Q,Li K,et al. Uptake,translocation and biotransformation of selenium nanoparticles in rice seedlings (Oryza sativa L.)[J]. Nanobiotechnology,2020,18(1):103.
[22]Lanza M G D B,Silva V M,Montanha G S,et al. Assessment of selenium spatial distribution using μ-XFR in cowpea [Vigna unguiculata (L.) Walp.] plants:integration of physiological and biochemical responses[J]. Ecotoxicology and Environmental Safety,2021,207:111216.
[23]Li Y X,Zhu N L,Liang X J,et al. A comparative study on the accumulation,translocation and transformation of selenite,selenate,and SeNPs in a hydroponic-plant system[J]. Ecotoxicology and Environmental Safety,2020,189:109955.1-109955.7.
[24]邵树勋,Dernovics M,龙胜桥. 恩施富硒豆角中硒的形态研究[J]. 矿物学报,2015,35(增刊1):671.
[25]Chan Q L,Afton S E,Caruso J A. Selenium speciation profiles in selenite-enriched soybean (Glycine Max) by HPLC-ICPMS and ESI-ITMS[J]. Metallomics,2010,2(2):47-53.
[26]陈佳佳,李汛,吴沿友,等. 增施CO2条件下施硒对温室黄瓜产量及硒吸收累积的影响[J]. 江苏农业学报,2020,36(6):1503-1511.
[27]White P J. Selenium metabolism in plants[J]. Biochimica et Biophysica Acta- General Subjects,2018,1862(11):2333-2342.

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备注/Memo

备注/Memo:
收稿日期:2021-11-27
基金项目:2019年湖北省技术创新专项(重大项目)(编号:2019ABA113);粮食作物种质创新与遗传改良湖北省重点实验室开放课题(编号:2020lzjj09)。
作者简介:汪园(1996—),男,湖北荆州人,硕士研究生,主要从事豆科植物富硒机理研究。E-mail:2390446193@qq.com。
通信作者:程水源,博士,教授,主要从事硒资源评价与利用研究,E-mail:s-y-cheng@sina.com;李丽,博士,副教授,研究方向为植物资源与富硒机理研究,E-mail:lily7819@whpu.edu.cn。
更新日期/Last Update: 2022-11-20