|本期目录/Table of Contents|

[1]胡祥祥,许姣,宋梦瑶,等.红花CDPK基因家族的鉴定及表达分析[J].江苏农业科学,2023,51(4):39-46.
 Hu Xiangxiang,et al.Identification and expression analysis of CDPK gene family in Carthamus tinctorius[J].Jiangsu Agricultural Sciences,2023,51(4):39-46.
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红花CDPK基因家族的鉴定及表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第4期
页码:
39-46
栏目:
生物技术
出版日期:
2023-02-20

文章信息/Info

Title:
Identification and expression analysis of CDPK gene family in Carthamus tinctorius
作者:
胡祥祥1 许姣12 宋梦瑶12 朱畇昊12
1.河南中医药大学药学院,河南郑州 450046; 2.呼吸疾病诊疗与新药研发河南省协同创新中心,河南郑州 450046
Author(s):
Hu Xiangxianget al
关键词:
红花CDPK基因家族生物信息学分析表达分析染色体定位
Keywords:
-
分类号:
S567.21+9.01
DOI:
-
文献标志码:
A
摘要:
钙依赖性蛋白激酶(CDPK)是植物细胞内Ca2+信号识别及转导的重要媒介,在植物的生长发育以及逆境响应过程中起着重要的作用。本研究通过多种生物信息学的方法和手段,对鉴定出来的30个CDPK基因进行分析鉴定,结果显示30个CtCDPK基因所编码的氨基酸分子量为47 781.96~93 251.66 ku,等电点为5.09~9.27;基因结构预测结果表明,所有CtCDPK基因均含有6~13个外显子;染色体定位结果表明,30个CtCDPK基因分别定位在10条染色体上,其中8号染色体上最多,有5个,2号、4号染色体上没有;系统发育树分析结果显示将其分为6个亚家族;表达分析结果表明CtCDPK基因在不同组织、不同发育阶段中表达模式有所差异。
Abstract:
-

参考文献/References:

[1]Hrabak E M,Chan C W M,Gribskov M,et al. The Arabidopsis CDPK-SnRK superfamily of protein kinases[J]. Plant Physiology,2003,132(2):666-680.
[2]Zhang K,Han Y T,Zhao F L,et al. Genome-wide identification and expression analysis of the CDPK gene family in grape,Vitis spp[J]. BMC Plant Biology,2015,15:164.
[3]Cheng S H,Willmann M R,Chen H C,et al. Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family[J]. Plant Physiology,2002,129(2):469-485.
[4]Ludwig A A,Romeis T,Jones J D G. CDPK-mediated signalling pathways:specificity and cross-talk[J]. Journal of Experimental Botany,2004,55(395):181-188.
[5]Harmon A C,Gribskov M,Harper J F. CDPKs-a kinase for every Ca2+signal?[J]. Trends in Plant Science,2000,5(4):154-159.
[6]余琴鸯,尹恒,安利佳,等. 谷子逆境应答相关的钙依赖蛋白激酶基因SiCDPK1的克隆与表达[J]. 作物学报,2014,40(9):1531-1539.
[7]谷卫彬,藜大爵. 世界红花种植的籽油脂肪酸组分评价[J]. 植物与资源学报,2002,11 (1):17-19.
[8]Kumar S,Stecher G,Tamura K. MEGA7:molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology and Evolution,2016,33(7):1870-1874.
[9]瞿城,乐世俊,林航,等. 红花化学成分研究[J]. 中草药,2015,46(13):1872-1877.
[10]Liu W,Li W,He Q L,et al. Genome-wide survey and expression analysis of calcium-dependent protein kinase in Gossypium raimondii[J]. PLoS One,2014,9(6):e98189.
[11]林欢,段伟科,周怡,等. 辣椒CDPK基因家族的鉴定、进化与表达分析[J]. 核农学报,2021,35(1):7-17.
[12]赵娟,朱凯凯,范平桦,等. 薄壳山核桃和山核桃CDPK基因家族的鉴定及表达分析[J]. 农业生物技术学报,2022,30(3):442-456.
[13]张进,李建波,刘伯斌,等. 杨树CDPK基因家族的表达分析及功能预测[J]. 林业科学研究,2014,27(5):604-611.
[14]Chen C J,Chen H,He Y H,et al. TBtools,a Toolkit for Biologists integrating various biological data handling tools with a user-friendly interface[J]. bioRxiv,2018,DOI:10.1101/289660.
[15]Harper J F,Breton G,Harmon A. Decoding Ca2+signals through plant protein kinases[J]. Annual Review of Plant Biology,2004,55:263-288.
[16]Harmon A C,Gribskov M,Gubrium E,et al. The CDPK superfamily of protein kinases[J]. New Phytologist,2001,151(1):175-183.
[17]Qin F,Yu B Z,Li W Q. Heat shock protein 101 (HSP101) promotes flowering under nonstress conditions[J]. Plant Physiology,2021,186(1):407-419.
[18]郑赟,王昊宁,刘靖文,等. 牡丹花发育相关基因PsPI的克隆与表达分析[J]. 分子植物育种,2021,19(7):2185-2192.
[19]Estruch J J,Kadwell S,Merlin E,et al. Cloning and characterization of a maize pollen-specific calcium-dependent calmodulin-independent protein kinase[J]. Proceedings of the National Academy of Sciences of the United States of America,1994,91(19):8837-8841.
[20]Yoon G M,Dowd P E,Gilroy S,et al. Calcium-dependent protein kinase isoforms in petunia have distinct functions in pollen tube growth,including regulating polarity[J]. The Plant Cell,2006,18(4):867-878.
[21]麻浩,王爽,周亚丽. 植物中钙依赖蛋白激酶的研究进展[J]. 南京农业大学学报,2017,40(4):565-572.
[22]丁艳芬. 玉米ZmCPK11在ABA诱导的抗氧化防护中的功能分析[D]. 南京:南京农业大学,2012.
[23]Fedosejevs E T,Gerdis S A,Ying S,et al. The calcium-dependent protein kinase RcCDPK2 phosphorylates sucrose synthase at Ser11 in developing castor oil seeds[J]. The Biochemical Journal,2016,473(20):3667-3682.
[24]王娇娇,韩胜芳,李小娟,等. 钙依赖蛋白激酶(CDPKs)介导植物信号转导的分子基础[J]. 草业学报,2009,18(3):241-250.

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备注/Memo

备注/Memo:
收稿日期:2022-03-21
基金项目:国家自然科学基金(编号:81603232);国家重点研发计划(编号:2017YFC1702800);河南省重大科技专项(编号:171100310500);河南省科技攻关项目(编号:172102310539)。
作者简介:胡祥祥(1999—),女,河南信阳人,硕士研究生,主要从事药用植物分子生物研究。E-mail:212725252@qq.com。
通信作者:朱畇昊,博士,副教授,主要从事药用植物分子生物研究。E-mail:guxinhan123@163.com。
更新日期/Last Update: 2023-02-20