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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第51卷

期数:

2023年第18期

页码:

45-53

栏目:

生物技术

出版日期:

2023-09-20

文章信息/Info

Title:

Identification，expression and response to low-salt stress of taurine transporter genes in Manalia clam(Ruditapes philippinarum)

作者:

张志超¹; 2; 王珺¹; 2; 霍忠明¹; 2; 聂鸿涛¹; 2; 闫喜武¹; 2; 丁鉴锋¹; 2

1.大连海洋大学水产与生命学院，辽宁大连 116023； 2.辽宁省贝类良种繁育工程技术研究中心，辽宁大连 116023

Author(s):

Zhang Zhichao; et al

关键词:

菲律宾蛤仔; 牛磺酸转运体; 低盐胁迫; 基因表达量

Keywords:

-

分类号:

S968.3

DOI:

-

文献标志码:

A

摘要:

牛磺酸转运体在动物牛磺酸代谢过程中发挥着重要作用，对基于基因组获取的菲律宾蛤仔牛磺酸转运体蛋白基因(RpTauT)进行鉴定、结构和组织表达分析，并对低盐胁迫过程中3种壳色蛤仔软体组织牛磺酸含量和水管组织RpTauT基因表达特征进行分析。鉴定出6条RpTauT基因(命名为RpTauT1~RpTauT6)，结构分析表明，其推测蛋白具有保守的12个跨膜结构域和钠离子结合位点；系统进化分析表明，蛤仔RpTauT与其他双壳贝类的遗传距离较近；组织表达分析表明，蛤仔RpTauT在外套膜组织和水管组织中表达量高，在性腺和唇瓣组织中表达量低。橙蛤软体部低盐胁迫24 h牛磺酸含量显著升高(P<0.05)；3种壳色蛤仔的RpTauT表达量均表现出先升高后降低的趋势，提示3种壳色蛤仔RpTauT基因表达量在低盐胁迫过程中表现出的差异性与不同壳色菲律宾蛤仔抗逆性差异有关。

Abstract:

-

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相似文献/References:

[1]杨晴晴,陈悦,李伟,等.菲律宾蛤仔凝集素在小黄鱼保鲜中的应用研究[J].江苏农业科学,2018,46(20):219.
　Yang Qingqing,et al.Effect of a lectin from manila clam Ruditapes philippinarum on preservation of Pseudosciaena polyactis[J].Jiangsu Agricultural Sciences,2018,46(18):219.

备注/Memo

备注/Memo:

收稿日期：2022-12-06
基金项目：辽宁省科学技术计划(编号：2020-MS-279)；国家重点研发计划(编号：2018YFD0901404)。
作者简介：张志超(1999—)，男，硕士研究生，主要从事贝类遗传育种研究。E-mail：1216022793@qq.com。
通信作者：丁鉴锋，博士，副教授，主要从事贝类遗传育种研究。E-mail：jfding@dlou.edu.cn。

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更新日期/Last Update: 2023-09-20