[1]李春秀,李勋兰,孙海艳,等.柑橘果实萜类色素和挥发物生物合成研究进展[J].江苏农业科学,2021,49(12):20-28.
　Li Chunxiu,et al.Research progress on biosynthesis of terpenoid pigments and volatiles of citrus fruit[J].Jiangsu Agricultural Sciences,2021,49(12):20-28.
点击复制

柑橘果实萜类色素和挥发物生物合成研究进展()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第12期
页码:
20-28
栏目:
专论与综述
出版日期:
2021-06-20

文章信息/Info

Title:
Research progress on biosynthesis of terpenoid pigments and volatiles of citrus fruit
作者:
李春秀1; 2; 李勋兰2; 孙海艳1; 梁国鲁1; 向素琼1; 韩国辉2
1.西南大学园艺园林学院，重庆 400716； 2.重庆市农业科学院果树研究所，重庆 401329
Author(s):
Li Chunxiu; et al
关键词:
柑橘; 果实; 类胡萝卜素; 萜类挥发物; 萜类色素
Keywords:
-
分类号:
S666.01
DOI:
-
文献标志码:
A
摘要:
柑橘果实的色泽和香气品质主要由果实中的萜类色素和挥发物的种类和含量决定。2-C-甲基-D-赤藓醇-4-磷酸(MEP)和甲羟戊酸(MVA)途径合成共同前体异戊烯焦磷酸和二甲基丙烯焦磷酸，经八氢番茄红素合成酶和萜类合成酶的作用分别进入萜类色素和挥发物的合成代谢。本文介绍了柑橘果实萜类色素的组成及呈色特点、萜类挥发物的组成及香气特点，综述了萜类色素和挥发物的生物合成途径及关键基因、主要的转录调控因子和环境调控因子的研究现状，对该领域未来的研究方向提出了展望，为改善柑橘果实色泽和香气品质提供了参考。
Abstract:
-

参考文献/References:

［1］丁晓波，张华，刘世尧，等. 柑橘果品营养学研究现状［J］. 园艺学报，2012，39(9)：1687-1702.
［2］Zheng H W，Zhang Q W，Quan J P，et al. Determination of sugars，organic acids，aroma components，and carotenoids in grapefruit pulps［J］. Food Chemistry，2016，205：112-121.
［3］Rodrigo M J，Alquézar B，Alós E，et al. Biochemical bases and molecular regulation of pigmentation in the peel of Citrus fruit［J］. Scientia Horticulturae，2013，163：46-62.
［4］Alquezar B，Rodrigo M J，Lado J，et al. A comparative physiological and transcriptional study of carotenoid biosynthesis in white and red grapefruit (Citrus paradisi Macf.)［J］. Tree Genetics & Genomes，2013，9(5)：1257-1269.
［5］Chen C X. Pigments in fruits and vegetables［M］. New York：Springer，2015.
［6］Wei X，Hu H，Tong H R，et al. Profiles of gene family members related to carotenoid accumulation in Citrus genus［J］. Journal of Plant Biology，2017，60(1)：1-10.
［7］Sawamura M，Kuriyama T. Quantitative determination of volatile constituents in the pummelo (Citrus grandis Osbeck forma Tosa-buntan)［J］. Journal of Agricultural and Food Chemistry，1988，36(3)：567-569.
［8］Lota M L，Serra D D，Tomi F，et al. Volatile components of peel and leaf oils of lemon and lime species［J］. Journal of Agricultural and Food Chemistry，2002，50(4)：796-805.
［9］Hgnadóttir A，Rouseff R L. Identification of aroma active compounds in orange essence oil using gas chromatography-olfactometry and gas chromatography-mass spectrometry［J］. Journal of Chromatography A，2003，998(1/2)：201-211.
［10］Miyazaki T，Plotto A，Baldwin E A，et al. Aroma characterization of tangerine hybrids by gas-chromatography-olfactometry and sensory evaluation［J］. Journal of the Science of Food and Agriculture，2012，92(4)：727-735.
［11］Zhang H P，Xie Y X，Liu C H，et al. Comprehensive comparative analysis of volatile compounds in citrus fruits of different species［J］. Food Chemistry，2017，230：316-326.
［12］Kato M，Ikoma Y，Matsumoto H，et al. Accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in citrus fruit［J］. Plant Physiology，2004，134(2)：824-837.
［13］Fanciullino A L，Dhuique-Mayer C，Luro F，et al. Carotenoid diversity in cultivated citrus is highly influenced by genetic factors［J］. Journal of Agricultural and Food Chemistry，2006，54(12)：4397-4406.
［14］Agócs A，Nagy V，Szabó Z，et al. Comparative study on the carotenoid composition of the peel and the pulp of different citrus species［J］. Innovative Food Science & Emerging Technologies，2007，8(3)：390-394.
［15］Bartley G，Scolnik P A. Plant carotenoids：pigments for photoprotection，visual attraction，and human health［J］. The Plant Cell，1995，7(7)：1027-1038.
［16］陶俊. 柑橘果实类胡萝卜素形成及调控的生理机制研究［D］. 杭州：浙江大学，2002.
［17］Finley J W，de Man J M，Lee C Y. Color and food colorants［M］//Principles of Food Chemistry. Berlin：Springer，2018.
［18］Lado J，Gambetta G，Zacarias L. Key determinants of citrus fruit quality：metabolites and main changes during maturation［J］. Scientia Horticulturae，2018，233：238-248.
［19］Allegrone G，Belliardo F，Cabella P. Comparison of volatile concentrations in hand-squeezed juices of four different lemon varieties［J］. Journal of Agricultural and Food Chemistry，2006，54(5)：1844-1848.
［20］Rouseff R L，Ruiz Perez-Cacho P，Jabalpurwala F. Historical review of citrus flavor research during the past 100 years［J］. Journal of Agricultural and Food Chemistry，2009，57(18)：8115-8124.
［21］Liu Y Q，Heying E，Tanumihardjo S A. History，global distribution，and nutritional importance of citrus fruits［J］. Comprehensive Reviews in Food Science and Food Safety，2012，11(6)：530-545.
［22］Wei X，Song M，Chen C X，et al. Juice volatile composition differences between Valencia orange and its mutant Rohde Red Valencia are associated with carotenoid profile differences［J］. Food Chemistry，2018，245：223-232.
［23］Perez-Cacho P R，Rouseff R. Processing and storage effects on orange juice aroma：a review［J］. Journal of Agricultural and Food Chemistry，2008，56(21)：9785-9796.
［24］Cheong M W，Liu S Q，Yeo J，et al. Identification of aroma-active compounds in Malaysian pomelo［Citrus grandis (L.) Osbeck］peel by gas chromatography-olfactometry［J］. Journal of Essential Oil Research，2011，23(6)：34-42.
［25］何朝飞，冉玥，曾林芳，等. 柠檬果皮香气成分的GC-MS分析［J］. 食品科学，2013，34(6)：175-179.
［26］Mahattanatawee K，Rouseff R，Valim M F，et al. Identification and aroma impact of norisoprenoids in orange juice［J］. Journal of Agricultural and Food Chemistry，2005，53(2)：393-397.
［27］El H A，Zhang F J，Wu F F，et al. Advances in fruit aroma volatile research［J］. Molecules，2013，18(7)：8200-8229.
［28］Liu C H，Cheng Y J，Zhang H Y，et al. Volatile constituents of wild citrus Mangshanyegan (Citrus nobilis Lauriro) peel oil［J］. Journal of Agricultural and Food Chemistry，2012，60(10)：2617-2628.
［29］Zhang W L，Chen T T，Tang J M，et al. Tracing the production area of citrus fruits using aroma-active compounds and their quality evaluation models［J］. Journal of the Science of Food and Agriculture，2020，100(2)：517-526.
［30］Goff S，Klee H J. Plant volatile compounds：sensory cues for health and nutritional value？［J］. Science，2006，311(5762)：815-819.
［31］Baldwin I T. Plant volatiles［J］. Current Biology，2010，20(9)：392-397.
［32］Pulido P，Perello C，Rodriguez-Concepcion M. New insights into plant isoprenoid metabolism［J］. Molecular Plant，2012，5(5)：964-967.
［33］Sun T H，Yuan H，Cao H B，et al. Carotenoid metabolism in plants：the role of plastids［J］. Molecular Plant，2018，11(1)：58-74.
［34］Nisar N，Li L，Lu S，et al. Carotenoid metabolism in plants［J］. Molecular Plant，2015，8(1)：68-82.
［35］Qualley A，Dudareva N. Plant volatiles［EB/OL］. (2010-09-15)［2020-09-17］. https：//onlinelibrary.wiley.com/doi/abs/10.1002/9780470015902.a0000910.pub2.
［36］Alquézar B，Rodríguez A，de la Pea M，et al. Genomic analysis of terpene synthase family and functional characterization of seven sesquiterpene synthases from Citrus sinensis［J］. Frontiers in Plant Science，2017，8：1481.
［37］Young P R，Lashbrooke J G，Alexandersson E，et al. The genes and enzymes of the carotenoid metabolic pathway in Vitis vinifera L.［J］. BMC Genomics，2012，13(1)：1-17.
［38］Ikoma Y，Komatsu A，Kita M，et al. Expression of a phytoene synthase gene and characteristic carotenoid accumulation during citrus fruit development［J］. Physiologia Plantarum，2001，111(2)：232-238.
［39］Peng G，Wang C Y，Song S，et al. The role of 1-deoxy-d-xylulose-5-phosphate synthase and phytoene synthase gene family in citrus carotenoid accumulation［J］. Plant Physiology and Biochemistry，2013，71：67-76.
［40］Bartley G，Viitanen P V，Pecker I，et al. Molecular cloning and expression in photosynthetic bacteria of a soybean cDNA coding for phytoenedesaturase，an enzyme of the carotenoid biosynthesis pathway［J］. Proceedings of the National Academy of Sciences of the United States of America，1991，88(15)：6532-6536.
［41］Hugueney P，Rmer S，Kuntz M，et al. Characterization and molecular cloning of a flavoprotein catalyzing the synthesis of phytofluene and ζ-carotene in Capsicum chromoplasts［J］. European Journal of Biochemistry，1992，209(1)：399-407.
［42］Giuliano G，Bartley G，Scolnik P A. Regulation of carotenoid biosynthesis during tomato development［J］. The Plant Cell，1993，5(4)：379-387.
［43］Li Z H，Matthews P D，Burr B，et al. Cloning and characterization of a maize cDNA encoding phytoenedesaturase，an enzyme of the carotenoid biosynthetic pathway［J］. Plant Molecular Biology，1996，30(2)：269-279.
［44］Kita M，Komatsu A，Omura M，et al. Cloning and expression of CitPDS1，a gene encoding phytoenedesaturase in citrus［J］. Bioscience Biotechnology and Biochemistry，2001，65(6)：1424-1428.
［45］Alquezar B，Rodrigo M J，Zacarías L. Regulation of carotenoid biosynthesis during fruit maturation in the red-fleshed orange mutant Cara Cara［J］. Phytochemistry，2008，69(10)：1997-2007.
［46］Rodrigo M J，Lado J，Alós E，et al. A mutant allele of ζ-carotene isomerase (Z-ISO) is associated with the yellow pigmentation of the “Pinalate” sweet orange mutant and reveals new insights into its role in fruit carotenogenesis［J］. BMC Plant Biology，2019，19(1)：1-16.
［47］Bai L，Kim E H，Dellapenna D，et al. Novel lycopene epsilon cyclase activities in maize revealed through perturbation of carotenoid biosynthesis［J］. The Plant Journal，2009，59(4)：588-599.
［48］Chaudhary N，Nijhawan A，Khurana J P，et al. Carotenoid biosynthesis genes in rice：structural analysis，genome-wide expression profiling and phylogenetic analysis［J］. Molecular Genetics and Genomics，2010，283(1)：13-33.
［49］Bramley P M. Regulation of carotenoid formation during tomato fruit ripening and development［J］. Journal of Experimental Botany，2002，53(377)：2107-2113.
［50］Ampomah-Dwamena C，Mcghie T，Wibisono R，et al. The kiwifruit lycopene β-cyclase plays a significant role in carotenoid accumulation in fruit［J］. Journal of Experimental Botany，2009，60(13)：3765-3779.
［51］Blas A L，Ming R，Liu Z，et al. Cloning of the papaya chromoplast-specific lycopene β-cyclase，CpCYC-b，controlling fruit flesh color reveals conserved microsynteny and a recombination hot spot［J］. Plant Physiology，2010，152(4)：2013-2022.
［52］Fu X M，Kong W B，Peng G，et al. Plastid structure and carotenogenic gene expression in red-and white-fleshed loquat (Eriobotrya japonica) fruits［J］. Journal of Experimental Botany，2012，63(1)：341-354.
［53］Alquézar B，Zacarías L，Rodrigo M J. Molecular and functional characterization of a novel chromoplast-specific lycopene β-cyclase from citrus and its relation to lycopene accumulation［J］. Journal of Experimental Botany，2009，60(6)：1783-1797.
［54］徐昌杰，张上隆. 柑橘类胡萝卜素合成关键基因研究进展［J］. 园艺学报，2002，29(增刊1)：619-623.
［55］Zhang L C，Ma G，Shirai Y，et al. Expression and functional analysis of two lycopene β-cyclases from citrus fruits［J］. Planta，2012，236(4)：1315-1325.
［56］Wei X，Chen C X，Yu Q B，et al. Novel expression patterns of carotenoid pathway-related genes in citrus leaves and maturing fruits［J］. Tree Genetics & Genomes，2014，10(3)：439-448.
［57］Joyard J，Ferro M，Masselon C，et al. Chloroplast proteomics and the compartmentation of plastidialisoprenoid biosynthetic pathways［J］. Molecular Plant，2009，2(6)：1154-1180.
［58］Ma G，Zhang L C，Yungyuen W，et al. Expression and functional analysis of citrus carotene hydroxylases：unravelling the xanthophyll biosynthesis in citrus fruits［J］. BMC Plant Biology，2016，16(1)：1-12.
［59］Auldridge M E，Mccarty D R，Klee H J. Plant carotenoid cleavage oxygenases and their apocarotenoid products［J］. Current Opinion in Plant Biology，2006，9(3)：315-321.
［60］Schwartz S H，Tan B C，Da G G，et al. Specific oxidative cleavage of carotenoids by VP14 of maize［J］. Science，1997，276(5320)：1872-1874.
［61］Rodrigo M J，Alquézar B，Alós E，et al. A novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments［J］. Journal of Experimental Botany，2013，64(14)：4461-4478.
［62］Kato M，Matsumoto H，Ikoma Y，et al. The role of carotenoid cleavage dioxygenases in the regulation of carotenoid profiles during maturation in citrus fruit［J］. Journal of Experimental Botany，2006，57(10)：2153-2164.
［63］Simkin A J，Schwartz S H，Auldridge M，et al. The tomato carotenoid cleavage dioxygenase 1 genes contribute to the formation of the flavor volatiles β-ionone，pseudoionone，and geranylacetone［J］. The Plant Journal，2004，40(6)：882-892.
［64］García-Limones C，Schnbele K，Blanco-Portales R，et al. Functional characterization of FaCCD1：a carotenoid cleavage dioxygenase from strawberry involved in lutein degradation during fruit ripening［J］. Journal of Agricultural and Food Chemistry，2008，56(19)：9277-9285.
［65］刘盛雨，章世奎，卢娟芳，等. 杏香气形成特异基因PaCCD1的克隆与功能分析［J］. 园艺学报，2018，45(3)：471-481.
［66］Huang F C，Molnár P，Schwab W. Cloning and functional characterization of carotenoid cleavage dioxygenase 4 genes［J］. Journal of Experimental Botany，2009，60(11)：3011-3022.
［67］Ma G，Zhang L C，Matsuta A，et al. Enzymatic formation of β-citraurin from β-cryptoxanthin and Zeaxanthin by carotenoid cleavage dioxygenase4 in the flavedo of citrus fruit［J］. Plant Physiology，2013，163(2)：682-695.
［68］Zheng X J，Zhu K J，Sun Q，et al. Natural variation in CCD4 promoter underpins species-specific evolution of red coloration in citrus peel［J］. Molecular Plant，2019，12(9)：1294-1307.
［69］Nieuwenhuizen N J，Green S A，Chen X，et al. Functional genomics reveals that a compact terpene synthase gene family can account for terpene volatile production in apple［J］. Plant Physiology，2013，161(2)：787-804.
［70］Falara V，Akhtar T A，Nguyen T T，et al. The tomato terpene synthase gene family［J］. Plant Physiology，2011，157(2)：770-789.
［71］Lücker J，El Tamer M K，Schwab W，et al. Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases［J］. European Journal of Biochemistry，2002，269(13)：3160-3171.
［72］Shimada T，Endo T，Fujii H，et al. Isolation and characterization of (E)-β-ocimene and 1，8 cineole synthases in Citrus unshiu Marc［J］. Plant Science，2005，168(4)：987-995.
［73］Shimada T，Endo T，Fujii H，et al. Characterization of three linalool synthase genes from Citrus unshiu Marc. and analysis of linalool-mediated resistance against Xanthomonas citri subsp. citri and Peniciliumitalicum in citrus leaves and fruits［J］. Plant Science，2014，229：154-166.
［74］Li W Y，Liu C H，He M，et al. Largely different contents of terpenoids in beef red-flesh tangerine and its wild type［J］. BMC Plant Biology，2017，17(1)：1-12.
［75］Sharon-Asa L，Shalit M，Frydman A，et al. Citrus fruit flavor and aroma biosynthesis：isolation，functional characterization，and developmental regulation of Cstps1，a key gene in the production of the sesquiterpene aroma compound valencene［J］. The Plant Journal，2003，36(5)：664-674.
［76］Yu Q B，Plotto A，Baldwin E A，et al. Proteomic and metabolomic analyses provide insight into production of volatile and non-volatile flavor components in mandarin hybrid fruit［J］. BMC Plant Biology，2015，15(1)：76.
［77］Yu Q B，Huang M，Jia H G，et al. Deficiency of valencene in mandarin hybrids is associated with a deletion in the promoter region of the valencene synthase gene［J］. BMC Plant Biology，2019，19(1)：101.
［78］Lu S，Zhang Y，Zhu K，et al. The citrus transcription factor CsMADS6 modulates carotenoid metabolism by directly regulating carotenogenic genes［J］. Plant Physiology，2018，176(4)：2657-2676.
［79］Zhu F，Luo T，Liu C Y，et al. An R2R3-MYB transcription factor represses the transformation of α-and β-branch carotenoids by negatively regulating expression of CrBCH22 and CrNCED55 in flavedo of Citrus reticulate［J］. The New Phytologist，2017，216(1)：178-192.
［80］Shen S L，Yin X R，Zhang B，et al. CitAP2.10 activation of the terpene synthase CsTPS1 is associated with the synthesis of (+)-valencene in ‘Newhall’ orange［J］. Journal of Experimental Botany，2016，67(14)：4105-4115.
［81］Li X，Xu Y Y，Shen S L，et al. Transcription factor CitERF71 activates the terpene synthase gene CitTPS16 involved in the synthesis of E-geraniol in sweet orange fruit［J］. Journal of Experimental Botany，2017，68(17)：4929-4938.
［82］van Wyk A A，Huysamer M，Barry G H. Extended low-temperature shipping adversely affects rind colour of ‘Palmer Navel’sweet orange［Citrus sinensis (L.) Osb.］due to carotenoid degradation but can partially be mitigated by optimising post-shipping holding temperature［J］. Postharvest Biology and Technology，2009，53(3)：109-116.
［83］Zhou J Y，Sun C D，Zhang L L，et al. Preferential accumulation of orange-colored carotenoids in Ponkan (Citrus reticulata) fruit peel following postharvest application of ethylene or ethephon［J］. Scientia Horticulturae，2010，126(2)：229-235.
［84］Carmona L，Zacarías L，Rodrigo M J. Stimulation of coloration and carotenoid biosynthesis during postharvest storage of ‘Navelina’ orange fruit at 12 ℃［J］. Postharvest Biology and Technology，2012，74：108-117.
［85］Yungyuen W，Ma G，Zhang L C，et al. Regulation of carotenoid metabolism in response to different temperatures in Citrus juice sacs in vitro［J］. Scientia Horticulturae，2018，238：384-390.
［86］Luan Y T，Wang S S，Wang R Q，et al. Accumulation of red apocarotenoid β-citraurin in peel of a spontaneous mutant of huyou (Citrus changshanensis) and the effects of storage temperature and ethylene application［J］. Food Chemistry，2020，309：125705.
［87］Tao N G，Wang C F，Xu J，et al. Carotenoid accumulation in postharvest “Cara Cara” navel orange (Citrus sinensis Osbeck) fruits stored at different temperatures was transcriptionally regulated in a tissue-dependent manner［J］. Plant Cell Reports，2012，31(9)：1667-1676.
［88］Lu Q，Huang X J，Lv S Y，et al. Carotenoid profiling of red navel orange “Cara Cara” harvested from five regions in China［J］. Food Chemistry，2017，232：788-798.
［89］Lado J，Gurrea A，Zacarías L，et al. Influence of the storage temperature on volatile emission，carotenoid content and chilling injury development in Star Ruby red grapefruit［J］. Food Chemistry，2019，295：72-81.
［90］Lado J，Alós E，Manzi M，et al. Light regulation of carotenoid biosynthesis in the peel of Mandarin and sweet orange fruits［J］. Frontiers in Plant Science，2019，10：1288.
［91］Lado J，Cronje P，Alquézar B，et al. Fruit shading enhances peel color，carotenes accumulation and chromoplast differentiation in red grapefruit［J］. Physiologia Plantarum，2015，154(4)：469-484.
［92］姜启航. 套袋对柚果实类胡萝卜素代谢和品质的影响［D］. 武汉：华中农业大学，2019.
［93］Ma G，Zhang L C，Kato M，et al. Effect of blue and red LED light irradiation on β-cryptoxanthin accumulation in the flavedo of citrus fruits［J］. Journal of Agricultural and Food Chemistry，2012，60(1)：197-201.
［94］Ma G，Zhang L C，Kato M，et al. Effect of the combination of ethylene and red LED light irradiation on carotenoid accumulation and carotenogenic gene expression in the flavedo of citrus fruit［J］. Postharvest Biology and Technology，2015，99：99-104.
［95］Zhang L C，Ma G，Kato M，et al. Regulation of carotenoid accumulation and the expression of carotenoid metabolic genes in citrus juice sacs in vitro［J］. Journal of Experimental Botany，2012，63(2)：871-886.
［96］Davidovich-Rikanati R，Sitrit Y，Tadmor Y，et al. Enrichment of tomato flavor by diversion of the early plastidial terpenoid pathway［J］. Nature Biotechnology，2007，25(8)：899-901.
［97］Liu C H，He M，Wang Z，et al. Integrative analysis of terpenoid profiles and hormones from fruits of red-flesh citrus mutants and their wild types［J］. Molecules，2019，24(19)：3456.

相似文献/References:

[1]郭健,曹珂,朱更瑞,等.蟠桃与圆桃果实4种内源激素含量差异分析[J].江苏农业科学,2016,44(03):196.
　Guo Jian,et al.Differential analysis of contents of four endogenous hormones of flat peach and round peach[J].Jiangsu Agricultural Sciences,2016,44(12):196.
[2]葛晓霞.柑橘CitSERK-LIKE基因原核表达载体的构建与表达[J].江苏农业科学,2016,44(04):22.
　Ge Xiaoxia.Construction of prokaryotic expression vector and expression of CitSERK1-like gene[J].Jiangsu Agricultural Sciences,2016,44(12):22.
[3]罗旭璐,张德国,李永和,等.樟叶越橘阴干果实的营养成分[J].江苏农业科学,2014,42(01):242.
　Luo Xulu,et al.Nutrient components of dried fruit of Vaccinium dunalianum Wight[J].Jiangsu Agricultural Sciences,2014,42(12):242.
[4]王爱波.槭属2种植物果实形态特征与扩散特性的比较[J].江苏农业科学,2016,44(04):271.
　Wang Aibo.Comparison of fruit morphological and dispersal characteristics of two Acer species[J].Jiangsu Agricultural Sciences,2016,44(12):271.
[5]汤路昀,曾光.中国柑橘产业空间集聚与农民收入的关系[J].江苏农业科学,2016,44(04):535.
　Tang Luyun,et al.Relationship between Chinese citrus industrial agglomeration and famers income[J].Jiangsu Agricultural Sciences,2016,44(12):535.
[6]王晶晶,陈奇凌,李铭,等.不同氮磷钾配施对滴灌红枣果实及植株养分含量的影响[J].江苏农业科学,2014,42(04):117.
　Wang Jingjing,et al.Effects of different treatments of N，P and K on nutrient contents in plant and fruit of Zizyphus jujube under drip irrigation[J].Jiangsu Agricultural Sciences,2014,42(12):117.
[7]郏艳红,吉立柱.番茄脐腐病发生机理初步研究[J].江苏农业科学,2015,43(04):158.
　Jia Yanhong,et al.Preliminary study on mechanism of tomato navel rot[J].Jiangsu Agricultural Sciences,2015,43(12):158.
[8]张晓梅,刘红明,李进学,等.有机肥对火龙果不同批次果实生长与品质的影响[J].江苏农业科学,2016,44(08):228.
　Zhang Xiaomei,et al.Effects of organic fertilizers on fruit growth and quality of different batches of pitaya[J].Jiangsu Agricultural Sciences,2016,44(12):228.
[9]魏召新,朱世平,阳佳位,等.不对称PCR的引物浓度优化及在柑橘基因型分析上的应用[J].江苏农业科学,2016,44(11):46.
　Wei Zhaoxin,et al.Optimization of primer ratio in asymmetric polymerase chain reaction system and its application in citrus genotype analysis[J].Jiangsu Agricultural Sciences,2016,44(12):46.
[10]董艳,陈磊,张亚红.4种膨大剂对葡萄果实生长发育的影响[J].江苏农业科学,2017,45(17):135.
　Dong Yan,et al.Effects of four kinds of expanding agents on growth and development of grape[J].Jiangsu Agricultural Sciences,2017,45(12):135.
[11]李金强,李文云,杨胜安,等.贵州柑橘主栽品种果实品质特征分析[J].江苏农业科学,2013,41(12):170.
　Li Jinqiang,et al.Analysis of fruit quality characteristics of citrus main cultivars in Guizhou area[J].Jiangsu Agricultural Sciences,2013,41(12):170.

备注/Memo

备注/Memo:
收稿日期：2020-10-17
基金项目：重庆市技术创新与应用发展专项(编号：cstc2019jscx-msxmX0371)；重庆市农业发展专项(NKY-2019AB006、NKY-2020AC006)；重庆市基础与前沿创新专项(编号：cstc2016jcyjA0046)；重庆市特色水果产业技术体系项目［编号：2020(3)03］。
作者简介：李春秀(1996—)，女，重庆人，硕士研究生，主要从事果树遗传育种与生物技术研究。E-mail：li1378602852@163.com。
通信作者：韩国辉，男，博士，副研究员，主要从事果树遗传育种与生物技术研究，E-mail：hghui2007@126.com；向素琼，女，博士，研究员，主要从事果树遗传育种与生物技术研究，E-mail：xiangsq@swu.edu.cn。
更新日期/Last Update: 2021-06-20