|本期目录/Table of Contents|

[1]葛晓霞.柑橘CitSERK-LIKE基因原核表达载体的构建与表达[J].江苏农业科学,2016,44(04):22-24.
　Ge Xiaoxia.Construction of prokaryotic expression vector and expression of CitSERK1-like gene[J].Jiangsu Agricultural Sciences,2016,44(04):22-24.
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柑橘CitSERK-LIKE基因原核表达载体的构建与表达(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年04期
页码:
22-24
栏目:
生物技术
出版日期:
2016-04-25

文章信息/Info

Title:
Construction of prokaryotic expression vector and expression of CitSERK1-like gene
作者:
葛晓霞
武汉生物工程学院应用生物技术研究中心，湖北武汉 430415
Author(s):
Ge Xiaoxia
关键词:
柑橘; CitSERK1-like; 表达载体; 构建; 原核表达
Keywords:
-
分类号:
S188；S666.01
DOI:
-
文献标志码:
A
摘要:
用RT-PCR扩增伏令夏橙体细胞胚发生类受体蛋白激酶(somatic embryogenesis receptor-like kinase，SERK)基因ORF区全长，克隆入载体，酶切后亚克隆入原核表达载体，并用SDS-PAGE观察CitSERK-LIKE基因的原核表达情况。结果显示，成功构建了pET-CitSERK1-like原核表达重组质粒，该重组质粒在大肠杆菌中可经IPTG诱导表达分子量约为69 ku的融合蛋白，与预测蛋白一致。
Abstract:
-

参考文献/References:

［1］Zimmerman J L. Somatic embryogenesis：a model for early development in higher plants［J］. The Plant Cell，1993，5(10)：1411-1423.
［2］Schmidt E D，Guzzo F，Toonen M A，et al. A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos［J］. Development，1997，124(10)：2049-2062.
［3］Somleva M N，Schmidt E D L，de Vries S C. Embryogenic cells in Dactylis glomerata L. (Poaceae) explants identified by cell tracking and by SERK expression［J］. Plant Cell Reports，2000，19(7)：718-726.
［4］Nolan K E，Irwanto R R，Rose R J. Auxin up-regulates MtSERK1 expression in both Medicago truncatula root-forming and embryogenic cultures［J］. Plant Physiology，2003，133(1)：218-230.
［5］Nolan K E，Kurdyukov S，Rose R J. Expression of the SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 (SERK1) gene is associated with developmental change in the life cycle of the model legume Medicago truncatula［J］. Journal of Experimental Botany，2009，60(6)：1759-1771.
［6］Hu H，Xiong L，Yang Y. Rice SERK1 gene positively regulates somatic embryogenesis of cultured cell and host defense response against fungal infection［J］. Planta，2005，222(1)：107-117.
［7］Singla B，Khurana J P，Khurana P. Characterization of three somatic embryogenesis receptor kinase genes from wheat，Triticum aestivum［J］. Plant Cell Reports，2008，27(5)：833-843.
［8］Sharma S K，Millam S，Hein I，et al. Cloning and molecular characterisation of a potato SERK gene transcriptionally induced during initiation of somatic embryogenesis［J］. Planta，2008，228(2)：319-330.
［9］Shimada T，Hirabayashi T，Endo T，et al. Isolation and characterization of the somatic embryogenesis receptor-like kinase gene homologue (CitSERK1) from Citrus unshiu Marc.［J］. Scientia Horticulturae，2005，103(2)：233-238.
［10］Ge X X，Fan G E，Chai L J，et al. Cloning，molecular characterization and expression analysis of a SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE gene (CitSERK1-like) in Valencia sweet orange［J］. Acta Physiologiae Plantarum，2010，32(6)：1197-1207.
［11］Savona M，Mattioli R，Nigro S，et al. Two SERK genes are markers of pluripotency in Cyclamen persicum Mill［J］. Journal of Experimental Botany，2012，63(1)：471-488.
［12］Ma J，He Y，Hu Z，et al. Characterization and expression analysis of AcSERK2，a somatic embryogenesis and stress resistance related gene in pineapple［J］. Gene，2012，500(1)：115-123.
［13］Ma J，He Y H，Wu C H，et al. Cloning and molecular characterization of a SERK gene transcriptionally induced during somatic embryogenesis in Ananas comosus cv. Shenwan［J］. Plant Molecular Biology Reporter，2012，30(1)：195-203.
［14］Guo W W，Li D L，Duan Y X. Citrus transgenics：current status and prospects［J］. Transgenic Plant Journd，2007，1：202-209.
［15］Hecht V，Vielle-Calzada J P，Hartog M V，et al. The arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture［J］. Plant Physiology，2001，127(3)：803-816.
［16］Santos M O，Romano E，Vieira L S，et al. Suppression of SERK gene expression affects fungus tolerance and somatic embryogenesis in transgenic lettuce［J］. Plant Biology，2009，11(1)：83-89.
［17］Albrecht C，Russinova E，Hecht V，et al. The Arabidopsis thaliana SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASES1 and 2 control male sporogenesis［J］. The Plant Cell，2005，17(12)：3337-3349.
［18］Albertini E，Marconi G，Reale L，et al. SERK and APOSTART：candidate genes for apomixis in Poa pratensis［J］. Plant Physiology，2005，138(4)：2185-2199.
［19］Albrecht C，Russinova E，Kemmerling B，et al. Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE proteins serve brassinosteroid-dependent and-independent signaling pathways［J］. Plant Physiology，2008，148(1)：611-619.

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备注/Memo

备注/Memo:
收稿日期：2015-03-13
基金项目：国家自然科学基金青年基金(编号：31201611)。
作者简介：葛晓霞(1981—)，女，山西阳泉人，博士，讲师，研究方向为柑橘分子育种。Tel：(027)89645532；E-mail：gexiaoxia15@aliyun.com。
更新日期/Last Update: 2016-04-25