|本期目录/Table of Contents|

[1]宋晓兵,彭埃天,黄峰,等.柑橘黄龙病亚洲种RPA快速检测方法的建立[J].江苏农业科学,2021,49(22):137-140.
 Song Xiaobing,et al.Establishment of RPA rapid detection method for candidatus Liberibacter asiaticus[J].Jiangsu Agricultural Sciences,2021,49(22):137-140.
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柑橘黄龙病亚洲种RPA快速检测方法的建立(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第22期
页码:
137-140
栏目:
植物保护
出版日期:
2021-11-20

文章信息/Info

Title:
Establishment of RPA rapid detection method for candidatus Liberibacter asiaticus
作者:
宋晓兵彭埃天黄峰汤亚飞崔一平凌金锋
广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室,广东广州 510640
Author(s):
Song Xiaobinget al
关键词:
柑橘柑橘黄龙病亚洲种RPA快速检测
Keywords:
-
分类号:
S436.661.1+9
DOI:
-
文献标志码:
A
摘要:
黄龙病是世界柑橘产业的毁灭性病害,引起柑橘黄龙病的病原分为亚洲种、非洲种和美洲种共3个种,目前我国柑橘黄龙病均由亚洲种引起,建立一套快速检测技术对我国柑橘黄龙病的防控具有重要意义。本研究根据柑橘黄龙病亚洲种16S rDNA基因序列设计引物,建立了基于重组酶聚合酶等温扩增(RPA)的检测方法,并评价了该方法的灵敏度和检测准确性。结果表明,本研究所建立的柑橘黄龙病亚洲种RPA快速检测方法特异性强、灵敏度高、操作过程简便,检测灵敏度比常规PCR提高10倍,为基层农技人员开展柑橘黄龙病的快速检测提供了一种新方法。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2021-02-20
基金项目:国家重点研发计划(编号:2018YFD0201500、2017YFD0202000);广东省现代农业产业技术体系创新团队建设项目(编号:2020KJ108);广东省现代农业产业共性关键技术研发创新团队建设项目(编号:2020KJ134)。
作者简介:宋晓兵(1980—),男,山东胶南人,博士,副研究员,主要从事柑橘病害防控技术研究。E-mail:xbsong@126.com。
通信作者:彭埃天,研究员,主要从事果树病害防控技术研究。E-mail:pengait@163.com。
更新日期/Last Update: 2021-11-20