|本期目录/Table of Contents|

[1]纪艺红,李越,白雪,等.马铃薯DHN家族基因鉴定及干旱胁迫诱导表达分析[J].江苏农业科学,2023,51(14):58-64.
　Ji Yihong,et al.Genome-wide identification and drought stress-induced expression analysis of the DHN gene family in potatoes[J].Jiangsu Agricultural Sciences,2023,51(14):58-64.
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马铃薯DHN家族基因鉴定及干旱胁迫诱导表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第14期
页码:
58-64
栏目:
生物技术
出版日期:
2023-07-20

文章信息/Info

Title:
Genome-wide identification and drought stress-induced expression analysis of the DHN gene family in potatoes
作者:
纪艺红; 李越; 白雪; 温美沙; 刘畅; 许春江; 李雅飞; 王磊
河北北方学院，河北张家口 075000
Author(s):
Ji Yihong; et al
关键词:
全基因组; 马铃薯; 干旱胁迫; 组织特异性; 表达分析
Keywords:
-
分类号:
S532.03
DOI:
-
文献标志码:
A
摘要:
马铃薯遭遇干旱胁迫时，会导致产量下降、品质降低。用生物信息学的方法，对马铃薯DHN(StDHN)基因家族进行全基因组鉴定，对其理化性质、染色体分布等进行分析，利用荧光定量的方法，对其在干旱胁迫下和不同组织中的表达模式进行分析。结果表明，StDHN基因家族共鉴定出5个家族成员，分布于1号、2号和4号染色体上，理化性质分析表明氨基酸长度为80~243个，分子量为8 544.27~25 121.94 ku，等电点(pI)的范围为5.24~7.38。StDHN蛋白的二级结构基本以无规卷曲为主，延伸链与β-转角的比例相当，仅有PG0009968以α-螺旋为主。对马铃薯DHN基因上游1 500 bp启动子区域顺式作用元件分析发现，DHN基因受到光的调控，平均每个基因中有10.4个光响应元件；还受到脱落酸(PG0015495、PG0003531、PG0009968)、赤霉素(PG0003531)等激素的调控；与逆境胁迫相关的包括低温响应元件(PG0030949、PG0003531、PG0009968)、干旱响应元件(PG0030949)等；生长发育调控元件包括胚乳表达(PG0015495)、种子特异性调控(PG0003531)、分生组织表达(PG0003531、PG0009968)。荧光定量结果发现，DHN基因参与干旱应答响应，也有可能参与马铃薯的生长调控。本研究为马铃薯中的耐旱候选基因研究提供参考。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2022-10-12
基金项目：国家现代农业产业技术体系建设专项(编号：CARS-09-P05)；河北省张家口市基础研究和人才培养计划项目（编号：2221015A）；河北省教育厅自然科学类青年项目(编号：QN2021011)；河北省马铃薯产业协同创新中心项目(编号：［2016］5号)。
作者简介：纪艺红(1990—)，女，河北张家口人，硕士，助理研究员，研究方向为分子抗逆育种。E-mail：281030822@qq.com。
通信作者：王磊，博士，讲师，研究方向为马铃薯种质资源创新。E-mail：wanglei@hebeinu.edu.cn。
更新日期/Last Update: 2023-07-20