|本期目录/Table of Contents|

[1]韩蕾蕾,张乐乐,王慧娟,等.丛枝菌根真菌对镉胁迫小麦褪黑素代谢的调节作用[J].江苏农业科学,2023,51(17):59-67.
　Han Lielei,et al.Regulation of melatonin metabolism in cadmium-stressed wheat by arbuscular mycorrhizal fungi [JY。]Han Lielei，et al(59)[J].Jiangsu Agricultural Sciences,2023,51(17):59-67.
点击复制

丛枝菌根真菌对镉胁迫小麦褪黑素代谢的调节作用(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第17期
页码:
59-67
栏目:
遗传育种与耕作栽培
出版日期:
2023-09-05

文章信息/Info

Title:
Regulation of melatonin metabolism in cadmium-stressed wheat by arbuscular mycorrhizal fungi [JY。]Han Lielei，et al(59)
作者:
韩蕾蕾; 张乐乐; 王慧娟; 腾慧鑫; 袁祖丽
河南农业大学生命科学学院，河南郑州 450002
Author(s):
Han Lielei; et al
关键词:
褪黑素; 镉; 丛枝菌根真菌; 小麦; 抗氧化
Keywords:
-
分类号:
S512.101
DOI:
-
文献标志码:
A
摘要:
为研究丛枝菌根真菌(AMF)是否通过对镉(Cd)胁迫小麦褪黑素(MLT)代谢的调节作用来增强小麦Cd耐性，以小麦和摩西球囊霉为试验材料，采用土培方法，研究Cd胁迫下接种AMF对小麦MLT代谢的调节作用。结果显示，Cd胁迫下接种AMF处理与同浓度Cd胁迫处理相比，小麦叶片5-羟色胺(5-HT)、5-甲氧基色胺(5-MT)、MLT及2-羟基褪黑素(2-HMT)含量均有不同程度的增加，MLT合成相关酶及分解相关酶的活性均有不同程度的提高，MLT的合成酶基因TaASMT1、TaASMT2、TaCOMT、TaSNAT及MLT的分解基因Ta2-ODD的表达水平均有不同程度上调。表明AMF可以通过上调Cd胁迫小麦内源MLT代谢相关基因表达水平来提高相应酶的活性，促进MLT的合成，同时加速MLT分解为2-HMT，缓解Cd胁迫造成的氧化伤害，增强小麦的Cd耐性。
Abstract:
-

参考文献/References:

［1］李建凤.土壤重金属污染现状及检测技术分析［J］. 化工设计通讯，2022，48(2)：193-195.
［2］宋伟，陈百明，刘琳.中国耕地土壤重金属污染概况［J］. 水土保持研究，2013，20(2)：293-298.
［3］Li J R，Xu Y M.Immobilization remediation of Cd-polluted soil with different water condition［J］. Journal of Environmental Management，2017，193：607-612.
［4］张芬琴，张红晓，沈振国.Cd胁迫下不同耐性豆科植物根内活性氧产生与基因组DNA多态性变化［J］. 生态学杂志，2012，31(9)：2330-2336.
［5］Romero-Puertas M C，Palma J M，Gómez M，et al. Cadmium causes the oxidative modification of proteins in pea plants［J］. Plant，Cell & Environment，2002，25(5)：677-686.
［6］Hasan M K，Ahammed G J，Yin L L，et al. Melatonin mitigates cadmium phytotoxicity through modulation of phytochelatins biosynthesis，vacuolar sequestration，and antioxidant potential in Solanum lycopersicum L［J］. Frontiers in Plant Science，2015，6：601.
［7］Hall J L.Cellular mechanisms for heavy metal detoxification and tolerance［J］. Journal of Experimental Botany，2002，53(366)：1-11.
［8］Li H，Chang J J，Chen H J，et al. Exogenous melatonin confers salt stress tolerance to watermelon by improving photosynthesis and redox homeostasis［J］. Frontiers in Plant Science，2017，8：295.
［9］Hernández-Ruiz J，Arnao M.Relationship of melatonin and salicylic acid in biotic/abiotic plant stress responses［J］. Agronomy，2018，8(4)：33.
［10］Shida C，Castrucci A M L，Lamy-Freund M T.High melatonin solubility in aqueous medium［J］. Journal of Pineal Research，1994，16(4)：198-201.
［11］赵燕，王东华，赵曦阳.植物中褪黑素的研究进展［J］. 西北植物学报，2014，34(1)：196-205.
［12］Li G Z，Wang Y Y，Liu J，et al. Exogenous melatonin mitigates cadmium toxicity through ascorbic acid and glutathione pathway in wheat［J］. Ecotoxicology and Environmental Safety，2022，237：113533.
［13］Kaya C，Okant M，Ugurlar F，et al. Melatonin-mediated nitric oxide improves tolerance to cadmium toxicity by reducing oxidative stress in wheat plants［J］. Chemosphere，2019，225：627-638.
［14］Tan D X，Manchester L C，Terron M P，et al. One molecule，many derivatives：a never-ending interaction of melatonin with reactive oxygen and nitrogen species？［J］. Journal of Pineal Research，2007，42(1)：28-42.
［15］Tousi S，Zoufan P，Ghahfarrokhie A R.Alleviation of cadmium-induced phytotoxicity and growth improvement by exogenous melatonin pretreatment in mallow (Malva parviflora) plants［J］. Ecotoxicology and Environmental Safety，2020，206：111403.
［16］Sami A，Shah F A，Abdullah M，et al. Melatonin mitigates cadmium and aluminium toxicity through modulation of antioxidant potential in Brassica napus L.［J］. Plant Biology，2020，22(4)：679-690.
［17］Wu S Q，Wang Y，Zhang J K，et al. Exogenous melatonin improves physiological characteristics and promotes growth of strawberry seedlings under cadmium stress［J］. Horticultural Plant Journal，2021，7(1)：13-22.
［18］Arnao M B，Hernández-Ruiz J.Melatonin：a new plant hormone and/or a plant master regulator？［J］. Trends in Plant Science，2019，24(1)：38-48.
［19］Shi H T，Tan D X，Reiter R J，et al. Melatonin induces class A1 heat-shock factors (HSFA1s) and their possible involvement of thermotolerance in Arabidopsis［J］. Journal of Pineal Research，2015，58(3)：335-342.
［20］Back K，Tan D X，Reiter R J.Melatonin biosynthesis in plants：multiple pathways catalyze tryptophan to melatonin in the cytoplasm or chloroplasts［J］. Journal of Pineal Research，2016，61(4)：426-437.
［21］Galano A，Tan D X，Reiter R J. Melatonin and related compounds：chemical insights into their protective effects against oxidative stress［J］. Current Organic Chemistry，2017，21(20)：2077-2095.
［22］Prescott A G，John P.Dioxygenases：molecular structure and role in plant metabolism［J］. Annual Review of Plant Physiology and Plant Molecular Biology，1996，47：245-271.
［23］Byeon Y，Lee H Y，Hwang O J，et al. Coordinated regulation of melatonin synthesis and degradation genes in rice leaves in response to cadmium treatment［J］. Journal of Pineal Research，2015，58(4)：470-478.
［24］Wang J P，Yuan J H，Ren Q，et al. Arbuscular mycorrhizal fungi enhanced salt tolerance of Gleditsia sinensis by modulating antioxidant activity，ion balance and P/N ratio［J］. Plant Growth Regulation，2022，97(1)：33-49.
［25］鲁薇，杨秀蓉，谭焱，等. 干旱胁迫下AMF对葡萄扦插苗生长及养分吸收的影响［J］. 中国南方果树，2022，51(2)：129-133，138.
［26］Ghre V，Paszkowski U.Contribution of the arbuscular mycorrhizal symbiosis to heavy metal phytoremediation［J］. Planta，2006，223(6)：1115-1122.
［27］Zhan F D，Li B，Jiang M，et al. Arbuscular mycorrhizal fungi enhance antioxidant defense in the leaves and the retention of heavy metals in the roots of maize［J］. Environmental Science and Pollution Research，2018，25(24)：24338-24347.
［28］Begum N，Ahanger M A，Zhang L X.AMF inoculation and phosphorus supplementation alleviates drought induced growth and photosynthetic decline in Nicotiana tabacum by up-regulating antioxidant metabolism and osmolyte accumulation［J］. Environmental and Experimental Botany，2020，176：104088.
［29］Liu D，Zheng K Y，Wang Y，et al. Harnessing an arbuscular mycorrhizal fungus to improve the adaptability of a facultative metallophytic poplar (Populus yunnanensis) to cadmium stress：physiological and molecular responses［J］. Journal of Hazardous Materials，2022，424：127430.
［30］张华，孙纪全，包玉英.丛枝菌根真菌影响植物次生代谢产物的研究进展［J］. 农业生物技术学报，2015，23(8)：1093-1103.
［31］刘茵，孟自力.黄淮中部小麦干旱的发生规律及成因分析：以商丘市为例［J］. 安徽农业科学，2018，46(16)：164-166.
［32］沈凤斌.含镉小麦再敲土壤污染警钟［J］. 生态经济，2017，33(9)：10-13.
［33］Ke Q B, Ye Jun, Wang B M, et al. Melatonin mitigates salt stress in wheat seedlings by modulating polyamine metabolism［J］. Frontiers in Plant Science, 2018, 9: 914.
［34］Poghosyan G H，Mukhaelyan Z H，Vardevanyan P H. Influence of cadmium ions on growth and antioxidant system activity of wheat (Triticum aestivum L.) seedlings［J］. International Journal of Scientific Research in Environmental Sciences，2014，2(10)：371-378.
［35］Ma L，Huang Z L，Li S Y，et al. Melatonin and nitrogen applications modulate early growth and related physio-biochemical attributes in maize under Cd stress［J］. Journal of Soil Science and Plant Nutrition，2021，21(2)：978-990.
［36］Li H，Chen X W，Wu L，et al. Effects of arbuscular mycorrhizal fungi on redox homeostasis of rice under Cd stress［J］. Plant and Soil，2020，455(1/2)：121-138.
［37］Faria J M S，Pinto A P，Teixeira D，et al. Diversity of native arbuscular mycorrhiza extraradical mycelium influences antioxidant enzyme activity in wheat grown under Mn toxicity［J］. Bulletin of Environmental Contamination and Toxicology，2022，108(3)：451-456.
［38］He L，Li C Y，Liu R J.Indirect interactions between arbuscular mycorrhizal fungi and Spodoptera exigua alter photosynthesis and plant endogenous hormones［J］. Mycorrhiza，2017，27(6)：525-535.
［39］Campos C，Nobre T，Goss M J，et al. Transcriptome analysis of wheat roots reveals a differential regulation of stress responses related to arbuscular mycorrhizal fungi and soil disturbance［J］. Biology，2019，8(4)：93.
［40］Chen J，Zhang H Q，Zhang X L，et al. Arbuscular mycorrhizal symbiosis mitigates oxidative injury in black locust under salt stress through modulating antioxidant defence of the plant［J］. Environmental and Experimental Botany，2020，175：104034.
［41］Cui G J，Ai S Y，Chen K，et al. Arbuscular mycorrhiza augments cadmium tolerance in soybean by altering accumulation and partitioning of nutrient elements，and related gene expression［J］. Ecotoxicology and Environmental Safety，2019，171：231-239.
［42］Yang X，Qin J H，Li J C，et al. Upland rice intercropping with Solanum nigrum inoculated with arbuscular mycorrhizal fungi reduces grain Cd while promoting phytoremediation of Cd-contaminated soil［J］. Journal of Hazardous Materials，2021，406：124325.
［43］Sun S S，Wen D，Yang W Y，et al. Overexpression of caffeic acid O-methyltransferase 1 (COMT1) increases melatonin level and salt stress tolerance in tomato plant［J］. Journal of Plant Growth Regulation，2020，39(3)：1221-1235.
［44］Yan Y Y，Sun S S，Zhao N，et al. COMT1 overexpression resulting in increased melatonin biosynthesis contributes to the alleviation of carbendazim phytotoxicity and residues in tomato plants［J］. Environmental Pollution，2019，252：51-61.
［45］Wang L，Feng C，Zheng X D，et al. Plant mitochondria synthesize melatonin and enhance the tolerance of plants to drought stress［J］. Journal of Pineal Research，2017，63(3)：e12429.
［46］Byeon Y，Back K.Molecular cloning of melatonin 2-hydroxylase responsible for 2-hydroxymelatonin production in rice (Oryza sativa)［J］. Journal of Pineal Research，2015，58(3)：343-351.
［47］Yu Y，Teng Z W，Mou Z M，et al. Melatonin confers heavy metal-induced tolerance by alleviating oxidative stress and reducing the heavy metal accumulation in Exophiala pisciphila，a dark septate endophyte (DSE)［J］. BMC Microbiology，2021，21(1)：40.
［48］Gu Q，Chen Z P，Yu X L，et al. Melatonin confers plant tolerance against cadmium stress via the decrease of cadmium accumulation and reestablishment of microRNA-mediated redox homeostasis［J］. Plant Science，2017，261：28-37.
［49］Byeon Y，Tan D X，Reiter R J，et al. Predominance of 2-hydroxymelatonin over melatonin in plants［J］. Journal of Pineal Research，2015，59(4)：448-454.

相似文献/References:

[1]史景允,于伟红,梁秋生.蓖麻对镉污染土壤的修复潜力[J].江苏农业科学,2014,42(11):386.
　Shi Jingyun,et al(8).Potential repairing of cadmium contaminated soil by castor oil plant[J].Jiangsu Agricultural Sciences,2014,42(17):386.
[2]房春生,王帆,刘多,等.模拟酸雨对白菜体内铅、镉富集的影响[J].江苏农业科学,2013,41(08):323.
　Fang Chunsheng,et al.Effects of simulated acid rain on lead and cadmium accumulation in Brassica chinensis ssp. chinensis[J].Jiangsu Agricultural Sciences,2013,41(17):323.
[3]雷忻,郭兆权,延志莲,等.镉胁迫对泥鳅血清卵黄蛋白原的诱导作用[J].江苏农业科学,2013,41(09):321.
　Lei Xin,et al.Inductive effect of cadmium stress on serum vitellogenin in Misgurnus anguillicaudatus[J].Jiangsu Agricultural Sciences,2013,41(17):321.
[4]郭继斌,王莉,韩娇,等.联合浸提法测定土壤有效态镉[J].江苏农业科学,2016,44(03):369.
　Guo Jibin,et al.Determination of soil available Cd content by universal extraction method[J].Jiangsu Agricultural Sciences,2016,44(17):369.
[5]高华健,王玉祯,侯丹,等.锌调节镉胁迫水稻幼苗根系生长的生理机制[J].江苏农业科学,2013,41(12):48.
　Gao Huajian,et al.Physiological effect of zinc on growth of rice seedling roots under cadmium stress[J].Jiangsu Agricultural Sciences,2013,41(17):48.
[6]姚茹,黎小正.广西沿海主要贝类养殖区海水、表层沉积物及近江牡蛎体内重金属镉监测与评价[J].江苏农业科学,2014,42(01):316.
　Yao Ru,et al.Monitoring and comprehensive assessment of Cd in sea water，surface sediments and body of Crassostrea rivularis in main shellfish culture areas of Guangxi coastal waters[J].Jiangsu Agricultural Sciences,2014,42(17):316.
[7]王淑娟,刘文举,王立克,等.褪黑激素对雌性动物生殖系统调节作用的研究进展[J].江苏农业科学,2016,44(06):15.
　Wang Shujuan,et al.Research progress of regulating effect of mellocated atonin on female reproductive system[J].Jiangsu Agricultural Sciences,2016,44(17):15.
[8]刘标,尹红梅,陈薇,等.高效镉吸附菌株的筛选及生物学特性[J].江苏农业科学,2014,42(03):316.
　Liu Biao,et al.Screening and biological characteristics of strains with strong cadmium adsorption ability[J].Jiangsu Agricultural Sciences,2014,42(17):316.
[9]刘思思,高旋旋,胡竹青,等.镉诱导锦鲤肾脏氧化性DNA损伤及谷胱甘肽抗氧化系统改变[J].江苏农业科学,2016,44(02):272.
　Liu Sisi,et al.Cadmium induced renal oxidative DNA damage and glutathione antioxidant system change of brocade carp[J].Jiangsu Agricultural Sciences,2016,44(17):272.
[10]宋晓慧,陆引罡,何丹,等.烟草对镉的吸收及镉在亚细胞中的分布[J].江苏农业科学,2014,42(05):116.
　Song Xiaohui,et al.Absorbtion and subcellular distribution of cadmium in tobacco[J].Jiangsu Agricultural Sciences,2014,42(17):116.

备注/Memo

备注/Memo:
收稿日期：2022-12-04
基金项目：国家自然科学基金 (编号：31771730)。
作者简介：韩蕾蕾(1994—)，女，河南周口人，硕士，从事镉污染修复小麦研究。E-mail：1678125333@qq.com。
通信作者：袁祖丽，博士，教授，从事丛枝菌根真菌对土壤重金属污染作物的生理、分子解毒机制研究。E-mail：zuliyuan@126.com。
更新日期/Last Update: 2023-09-05