[1]黄强,姜伟业,舒婷.马铃薯NHX基因家族的鉴定与表达模式分析[J].江苏农业科学,2024,52(15):44-52.
　Huang Qiang,et al.Genome identification and expression analysis of NHX gene family in Solanum tuberosum[J].Jiangsu Agricultural Sciences,2024,52(15):44-52.
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马铃薯NHX基因家族的鉴定与表达模式分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第15期
页码:
44-52
栏目:
生物技术
出版日期:
2024-08-05

文章信息/Info

Title:
Genome identification and expression analysis of NHX gene family in Solanum tuberosum
作者:
黄强; 姜伟业; 舒婷
江西医学高等专科学校基础医学院，江西上饶 334000
Author(s):
Huang Qiang; et al
关键词:
马铃薯; 盐胁迫; NHX; 基因家族
Keywords:
-
分类号:
S532.01
DOI:
-
文献标志码:
A
摘要:
NHX基因是一类参与植物对盐胁迫逆境适应的基因家族，在植物对盐胁迫逆境的适应中发挥着重要的作用。为探究马铃薯NHX基因家族的功能和特征及在逆境适应中的潜在作用，从马铃薯基因组中通过生物信息学方法鉴定到7个马铃薯NHX基因，对这些家族成员进行理化性质分析、染色体定位分析、系统进化关系分析、启动子顺式调控元件分析、与miRNA的靶向关系分析、组织表达特异性、生物/非生物胁迫下的表达模式分析。结果表明，7个StNHX基因家族成员分布在1、4、6号染色体上；氨基酸长度为224~1 044个，蛋白分子量在24 698.2~116 236.32 u之间，等电点为在5.95~8.57，亲水系数为0.022~0.623。7个StNHX基因来源于3个邻近复制基因对和3个转座复制基因对，与拟南芥进行物种间共线性分析鉴定到5对直系同源基因对。系统进化分析发现，马铃薯NHX基因可以被分为3个亚群；启动子序列分析发现，StNHX基因含有多个与激素和胁迫响应相关的顺式作用元件。与miRNA靶向关系分析共预测到73个已知的miRNA可能调控7个StNHX基因。全部NHX家族成员在根和萼片中都有表达，StNHX3基因在各个组织中表达量最多，在生物和非生物胁迫下不同成员也呈现出不同的响应模式。本研究结果有助于增加对马铃薯NHX基因家族的了解，为进一步探索马铃薯NHX基因家族的功能和调控机制提供重要的参考。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2023-09-17
基金项目：江西省教育厅科学技术研究项目(编号：GJJ2205814)。
作者简介：黄强(1986—)，男，江西余干人，硕士，讲师，研究方向为生物化学与分子生物学。E-mail：hkzxhq2@163.com。
更新日期/Last Update: 2024-08-05