[1]勾朝金,杨明国,刘勉,等.内生真菌JG2的生物学特性和抑菌代谢产物研究[J].江苏农业科学,2025,53(12):153-160.
 Gou Chaojin,et al.Study on biological characterization and inhibitory metabolites of endophytic fungus JG2[J].Jiangsu Agricultural Sciences,2025,53(12):153-160.
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内生真菌JG2的生物学特性和抑菌代谢产物研究()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第12期
页码:
153-160
栏目:
植物保护
出版日期:
2025-06-20

文章信息/Info

Title:
Study on biological characterization and inhibitory metabolites of endophytic fungus JG2
作者:
勾朝金1杨明国1刘勉2刘坚2马云强2芦俊佳1
1.西南林业大学园林园艺学院,云南昆明 650224; 2.西南林业大学云南省森林灾害预警与控制重点实验室,云南昆明 650233
Author(s):
Gou Chaojinet al
关键词:
内生真菌生物学特性抑菌活性次级代谢产物
Keywords:
-
分类号:
S182
DOI:
-
文献标志码:
A
摘要:
为了解内生真菌JG2的生长特性及其对病原菌的生长抑制作用,对1株内生真菌菌株JG2进行生物学特性研究、平板抑菌试验及抑菌代谢产物测定。结果表明:(1)JG2菌株的最适生长pH值范围为5.0~9.0;在以甘露醇为碳源的培养基上生长情况为最佳;氯化铵为最佳生长氮源;最适生长温度为20 ℃;最适生长的光照条件为全光照;PDA培养基为最适生长培养基。(2)在平板抑菌试验中,JG2菌株与棒孢新拟盘多毛孢菌(Neopestalotiopsis clavispora)、睡莲刺盘孢菌(Colletotrichum nymphaeae)、细极链格孢菌(Alternaria tenuissima)以及瓜拟多隔孢菌(Stagonosporopsis cucurbitacearum)之间形成明显的抑菌带;在双平板抑菌试验中,JG2菌株的挥发性代谢产物对5株病原真菌具有不同程度的抑制作用,其中对细极链格孢菌的生长抑制效果最佳,抑制率高达76.51%,对多喙茎点霉(Phoma multirostrata)的生长抑制效果最弱,抑制率为19.63%。(3)抑菌代谢产物测定发现,菌株JG2产常见的黄酮类(flavonoids)、萜类(terpenoids )、生物碱类(alkaloids)、醌类(quinones)以及有机酸类(organic acids)等抑菌代谢产物,其中,有机酸类含量占比为21.82%、黄酮为5.40%、生物碱为3.73%、萜类为1.49%、醌类为0.44%。综上,表明JG2菌株具有良好的抑菌开发潜能。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2024-04-19
基金项目:国家自然科学基金(编号:32060695);云南省农业联合专项-面上项目(编号:202301BD070001-091、202101BD070001-128);云南省“兴滇英才支持计划”青年人才项目(编号:990123069)。
作者简介:勾朝金(2000—),女,贵州贵阳人,硕士研究生,主要从事园林园艺植物病害防治研究。E-mail:1293796190@qq.com。
通信作者:芦俊佳,博士,副教授,主要从事园林园艺植物病虫害综合治理研究。E-mail: 58826911@qq.com。
更新日期/Last Update: 2025-06-20