[1]杨庆,李旭,彭泽龙,等.枯草芽孢杆菌832用量对冬小麦生长发育、产量品质和土壤化学性质的影响[J].江苏农业科学,2025,53(14):78-85.
　Yang Qing,et al.Effects of application rates of Bacillus subtilis strain 832 on growth，yield，quality and soil chemical properties of winter wheat[J].Jiangsu Agricultural Sciences,2025,53(14):78-85.
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枯草芽孢杆菌832用量对冬小麦生长发育、产量品质和土壤化学性质的影响()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第14期
页码:
78-85
栏目:
遗传育种与耕作栽培
出版日期:
2025-07-20

文章信息/Info

Title:
Effects of application rates of Bacillus subtilis strain 832 on growth，yield，quality and soil chemical properties of winter wheat
作者:
杨庆1; 李旭1; 彭泽龙1; 李响1; 葛姗姗2; 高志岭1; 刘春敬1; 范玉婧1; 梁学优1; 李雨晴1
1.河北农业大学资源与环境科学学院/河北省农田生态环境重点实验室，河北保定 071000； 2.中国冶金地质总局地球物理勘查院，河北保定 071000
Author(s):
Yang Qing; et al
关键词:
枯草芽孢杆菌; 冬小麦; 土壤化学性质; 产量; 品质; 生长发育
Keywords:
-
分类号:
S512.1+10.6
DOI:
-
文献标志码:
A
摘要:
为明确不同枯草芽孢杆菌用量对华北地区冬小麦生长发育、产量品质和土壤化学性质的影响，在玉米—小麦轮作区开展田间试验，以枯草芽孢杆菌8-32(有效活菌数≥20×108 CFU/mL，芽孢率＞95%，IAA＞30 mg/L)为供试菌剂，采用浅埋滴灌的方式在冬小麦拔节期分别施用15 L/hm2(G1处理)、45 L/hm2(G3处理)、75 L/hm2(G5处理)和105 L/hm2(G7处理)的菌剂，以不施用菌剂为对照(CK)，研究施用菌剂8-32不同用量对土壤化学性质、冬小麦生长发育、产量、籽粒淀粉含量、蛋白质含量以及小麦赤霉病防控效果的影响。结果表明，与CK相比，各施用菌剂处理的有效磷和速效钾含量分别增加3.60%~38.79%和6.56%~21.31%，尤其是G5处理，其有效磷和速效钾含量显著高于CK(P<005)，菌剂8-32施用能够促进小麦植株的生长发育。在抽穗期，与CK相比，菌剂施用后株高、根干重和植株干重分别增加11.58%~21.50%、13.33%~26.67%和4.15%~56.99%。G1、G3、G5和G7的产量分别为7 065.15、7 118.46、7 985.72、7 257.36 kg/hm2，增产率分别为16.67%、17.55%、31.87%和19.84%，其中G5产量极显著高于CK(P<0.01)。处理G5的籽粒蛋白、直链淀粉和支链淀粉含量分别为13.17%、18.34%和49.01%，比CK分别高622%、20.44%和9.78%。菌剂8-32施用对小麦赤霉病也具有积极的防控效果，尤其是G5处理，其病穗防效达4783%，且其产投比最高，为1.82。
Abstract:
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参考文献/References:

［1］Hu X Y，Lei H M. Fifteen-year variations of water use efficiency over a wheat-maize rotation cropland in the North China Plain［J］. Agricultural and Forest Meteorology，2021，306：108430.
［2］Sim J X F，Doolette C L，Vasileiadis S，et al. Pesticide effects on nitrogen cycle related microbial functions and community composition［J］. Science of the Total Environment，2022，807(Pt1)：150734.
［3］Abbas M，Saleem M，Hussain D，et al. Review on integrated disease and pest management of field crops［J］. International Journal of Tropical Insect Science，2022，42(5)：3235-3243.
［4］Kvakkestad V，Steiro  L，Vatn A. Pesticide policies and farm behavior：the introduction of regulations for integrated pest management［J］. Agriculture，2021，11(9)：828-845.
［5］Chen Y H，Li S S，Liu N，et al. Effects of different types of microbial inoculants on available nitrogen and phosphorus，soil microbial community，and wheat growth in high-P soil［J］. Environmental Science and Pollution Research International，2021，28(18)：23036-23047.
［6］Md Gulzar A B，Mazumder P B. Plant growth promoting phyllobacteria：an effective tool for sustainable agriculture［J］. Russian Journal of Plant Physiology，2024，70(8)：196.
［7］Deng C，Liang X L，Zhang N，et al. Molecular mechanisms of plant growth promotion for methylotrophic Bacillus aryabhattai LAD［J］. Frontiers in Microbiology，2022，13：917382.
［8］Yang W，Zhao Y N，Yang Y，et al. Co-application of biochar and microbial inoculants increases soil phosphorus and potassium fertility and improves soil health and tomato growth［J］. Journal of Soils and Sediments，2023，23(2)：947-957.
［9］Dong Q Q，Liu Q X，Goodwin P H，et al. Isolation and genome-based characterization of biocontrol potential of Bacillus siamensis YB-1631 against wheat crown rot caused by Fusarium pseudograminearum［J］. Journal of Fungi，2023，9(5)：547-566.
［10］Nayak S K. Multifaceted applications of probiotic Bacillus species in aquaculture with special reference to Bacillus subtilis［J］. Reviews in Aquaculture，2021，13(2)：862-906.
［11］Xu W，Yang Q，Yang F，et al. Evaluation and genome analysis of Bacillus subtilis YB-04 as a potential biocontrol agent against Fusarium wilt and growth promotion agent of cucumber［J］. Frontiers in Microbiology，2022，13：885430.
［12］Zou L，Wang Q，Wu R X，et al. Biocontrol and plant growth promotion potential of endophytic Bacillus subtilis JY-7-2L on Aconitum carmichaelii Debx［J］. Frontiers in Microbiology，2023，13：1059549.
［13］Suriani，Sebayang A，Mirsam H，et al. Control of Fusarium verticillioides on corn with a combination of Bacillus subtilis TM3 formulation and botanical pesticides［J］. Saudi Journal of Biological Sciences，2021，28(12)：7000-7005.
［14］郑小亮，董超，牛瑞艳，等. 枯草芽孢杆菌Zl-2抗菌蛋白特性及对小麦赤霉病菌的抑制作用［J］. 黑龙江大学自然科学学报，2018，35(2)：206-211.
［15］Xu W，Yang Q，Xie X，et al. Genomic and phenotypic insights into the potential of Bacillus subtilis YB-15 isolated from rhizosphere to biocontrol against crown rot and promote growth of wheat［J］. Biology，2022，11(5)：778-791.
［16］Rathod K，Rana S，Dhandhukia P，et al. From Sea to Soil：Marine Bacillus subtilis enhancing chickpea production through in vitro and in vivo plant growth promoting traits［J］. Brazilian Journal of Microbiology，2024，55(1)：823-836.
［17］谷清义，王妍佳，李梦楠，等. 枯草芽孢杆菌BSCY-1对黄瓜种子萌发和幼苗生长特性的影响［J］. 北方园艺，2024(10)：17-23.
［18］王冬云，高峰，刘笑稳，等. 枯草芽孢杆菌8-32产吲哚乙酸发酵条件优化［J］. 扬州大学学报(农业与生命科学版)，2023，44(5)：127-134.
［19］贾田惠，杨可欣，时向哲，等. 枯草芽孢杆菌8-32产抗真菌物质发酵条件优化［J］. 河北农业大学学报，2019，42(1)：77-82，89.
［20］李晶. 区域农田生态系统生产力稳定性评价——以河北省雄县为例［D］. 保定：河北农业大学，2007：23-24.
［21］吴洪生，陈小青，马文舟，等. 磷石膏改良滨海盐土效果及对小麦生长的影响［J］. 土壤学报，2024，61(4)：1077-1087.
［22］鲍士旦. 土壤农化分析［M］. 3版.北京：中国农业出版社，2000：25-114.
［23］Tian C，Wang H M，Wu S F，et al. Exogenous microorganisms promote moss biocrust growth by regulating the microbial metabolic pathway in artificial laboratory cultivation［J］. Frontiers in Microbiology，2022，13：819888.
［24］Assainar S K，Abbott L K，Mickan B S，et al. Response of wheat to a multiple species microbial inoculant compared to fertilizer application［J］. Frontiers in Plant Science，2018，9：1601.
［25］陈芳，李字辉，王兵跃，等. 微生物菌剂对小麦生长发育及产量的影响［J］. 新疆农业科学，2023，60(10)：1-8.
［26］Tshering K，Rengel Z，Storer P，et al. Microbial consortium inoculum with rock minerals increased wheat grain yield，nitrogen-use efficiency，and protein yield due to larger root growth and architecture［J］. Agronomy，2022，12(10)：2481.
［27］Qi Y Q，Liu H L，Wang J H，et al. Effects of different straw biochar combined with microbial inoculants on soil environment in pot experiment［J］. Scientific Reports，2021，11(1)：14685.
［28］Zhao S P，Zhang Y，Tan M Y，et al. NnNF-YB1 induced by the potassium fertilizer enhances starch synthesis in rhizomes of Nelumbo nucifera［J］. Industrial Crops and Products，2023，203：117197.
［29］陈雨露，康娟，王家瑞，等. 灌水与施磷对小麦氮素积累运转及水分利用效率的影响［J］. 麦类作物学报，2019，39(9)：1095-1104.
［30］李其胜，殷小冬，董青君，等. 生物炭和微生物菌剂添加对菇渣好氧堆肥过程及其养分变化的影响［J］. 江苏农业科学，2023，51(11)：212-218.
［31］Prusty S，Sahoo R K，Sharaya R，et al. Unraveling the potential of native Azotobacter and Azospirillum spp.formulations for sustainable crop production of rice (Oryza sativa L.var.Khandagiri)［J］. South African Journal of Botany，2023，162：10-19.
［32］Dal Cortivo C，Barion G，Visioli G，et al. Increased root growth and nitrogen accumulation in common wheat following PGPR inoculation：assessment of plant-microbe interactions by ESEM［J］. Agriculture，Ecosystems & Environment，2017，247：396-408.
［33］常娜，张雪娇，马璐璐，等. 微生物菌剂对小麦生长及土传病害预防效果的影响［J］. 作物杂志，2017(1)：155-160.
［34］王冬云. 枯草芽孢杆菌8-32菌株促生作用及吲哚乙酸(IAA)合成途径解析［D］. 保定：河北农业大学，2023：9-29.
［35］Afzal A，Bahader S，Ul Hassan T，et al. Rock phosphate solubilization by plant growth-promoting Bacillus velezensis and its impact on wheat growth and yield［J］. Geomicrobiology Journal，2023，40(2)：131-142.
［36］郭成瑾，陈杭，王喜刚，等. 育苗基质添加复配微生物菌剂对番茄生长及根腐病发生的影响［J］. 北方园艺，2022(16)：52-57.
［37］Wang T H，Ahmad S，Yang L，et al. Preparation，biocontrol activity and growth promotion of biofertilizer containing Streptomyces aureoverticillatus HN6［J］. Frontiers in Plant Science，2022，13：1090689.

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　Yang Lin,et al.Retrieval of winter wheat phenological period based on MODIS NDVI data in Jiangsu Province[J].Jiangsu Agricultural Sciences,2016,44(14):315.

备注/Memo

备注/Memo:
收稿日期：2024-07-01
基金项目：国家重点研发计划(编号：2022YFD1901303)。
作者简介：杨庆(1999—)，女，河北保定人，硕士研究生，主要从事农业环境保护研究。E-mail：yangqing202402@163.com。
通信作者：刘春敬，博士，副教授，主要从事农业环境保护研究。E-mail：chunjingliu2008@163.com。
更新日期/Last Update: 2025-07-20