[1]王英琪,李景伟,刘福芳,等.高粱GLR基因家族鉴定与表达分析[J].江苏农业科学,2025,53(22):47-58.
　Wang Yingqi,et al.Identification and expression analysis of GLR gene family in sorghum[J].Jiangsu Agricultural Sciences,2025,53(22):47-58.
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高粱GLR基因家族鉴定与表达分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第22期
页码:
47-58
栏目:
生物技术
出版日期:
2025-11-20

文章信息/Info

Title:
Identification and expression analysis of GLR gene family in sorghum
作者:
王英琪; 李景伟; 刘福芳; 姜超
呼伦贝尔学院农学院，内蒙呼伦贝尔 021008
Author(s):
Wang Yingqi; et al
关键词:
高粱; GLR基因家族; 生物信息学; 表达分析; 干旱胁迫
Keywords:
-
分类号:
S188；S514.01
DOI:
-
文献标志码:
A
摘要:
植物谷氨酸受体基因(GLR)在植物的发育、信号传导途径以及对环境胁迫的响应中起着重要作用，为了解高粱GLR基因家族的特征和基本功能，通过生物信息学方法，开展GLR基因家族的鉴定，并进行理化性质、系统进化关系、基因结构、顺式作用元件分析和表达分析。结果表明，在高粱基因组中共鉴定出21个GLR基因，成员多为酸性蛋白，主要定位在质膜上，氨基酸数量介于685~1 012个之间，且理化性质具有显著差异；高粱GLR基因家族成员可分为4组，均由4~6个外显子组成，且不均匀地分布在高粱的4条染色体上；21个基因家族成员中有3个SbGLR形成2个片段重复的基因对，且和水稻共享10个线性基因；高粱GLR基因家族的启动子存在多个脱落酸、茉莉酸甲酯和低温响应等多种与非生物胁迫相关的顺式作用元件；表达分析显示，SbGLR3.4在叶片、花梗和根等营养组织中有很高的表达值，干旱胁迫处理后叶片表达量变化明显，转录组数据分析显示共有12个差异表达基因且显示出差异化的表达模式。本研究鉴定出21个GLR基因，其中SbGLR3.4、SbGLR1.3、SbGLR1.4和SbGLR1.6具有典型的GLR基因结构，且在干旱胁迫下差异性表达，可以作为候选基因进行深入的研究，为进一步研究高粱GLR基因家族的功能和高粱抵抗非生物胁迫的分子机制提供理论基础。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2024-10-26
基金项目：内蒙古自治区高等学校科学研究项目(编号：NJZY2122)；呼伦贝尔市科技计划(编号：NC2021002)；呼伦贝尔市“科技兴市”行动重点专项(成果转化)项目(编号：2022HZZX006)。
作者简介：王英琪(1989—)，女，内蒙古呼伦贝尔人，硕士，讲师，研究方向为作物分子育种。E-mail：chnwangyq@126.com。
通信作者：姜超，博士，副教授，研究方向为作物遗传育种。E-mail：jiangchao8905@sina.com。
更新日期/Last Update: 2025-11-20