|本期目录/Table of Contents|

[1]李祥栋,张明生,王洋,等.贵州优质酒用高粱Waxy基因的鉴定分析[J].江苏农业科学,2014,42(05):39-42.
　Li Xiangdong,et al.Identification and analysis of Waxy gene in brewing sorghum from Guizhou Province[J].Jiangsu Agricultural Sciences,2014,42(05):39-42.
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贵州优质酒用高粱Waxy基因的鉴定分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年05期
页码:
39-42
栏目:
生物技术
出版日期:
2014-05-25

文章信息/Info

Title:
Identification and analysis of Waxy gene in brewing sorghum from Guizhou Province
作者:
李祥栋1;  张明生1;  王洋1;  任明见1;  高翔2
1.贵州大学生命科学学院，贵州贵阳 550025； 2.贵州省农业科学院，贵州贵阳 550006
Author(s):
Li Xiangdong; et al
关键词:
高粱; Waxy基因; 淀粉; 粳糯性; 贵州省
Keywords:
-
分类号:
A
DOI:
-
文献标志码:
S514.01
摘要:
利用10个高粱品种(9个酒用高粱品种和1个非糯性品种)，在进行单籽粒粳糯性检测、淀粉含量测定的基础上，进一步对Waxy基因位点进行鉴定分析。结果表明，9个糯高粱品种糯性籽粒所占的比例基本上达到了100%；非糯性品种(BTx23)直链淀粉含量为13.83%，9个糯性品种的直链淀粉含量在0.18%～3.26%之间，与非糯性品种差异极显著；在所有糯高粱品种中有2个为wa突变类型，其余7个均为wb突变类型，非糯性品种属野生型(W)。
Abstract:
-

参考文献/References:

［1］Denyer K，Johnson P，Zeeman S，et al. The control of amylose synthesis［J］. Journal of Plant Physiology，2001，158(4)：479-487.
［2］Shure M，Wessler S，Fedoroff N. Molecular identification and isolation of the Waxy locus in maize［J］. Cell，1983，35(1)：225-233.
［3］Hirano H Y，Sano Y. Molecular characterization of the waxy locus of rice(Oryza sativa)［J］. Plant & Cell Physiology，1991，32(7)：989-997.
［4］Ainsworth C，Clark J，Balsdon J. Expression，organisation and structure of the genes encoding the Waxy protein(granule-bound starch synthase)in wheat［J］. Plant Molecular Biology，1993，22(1)：67-82.
［5］Rohde W，Becker D，Salamini F. Structural analysis of the Waxy locus from Hordeum vulgare［J］. Nucleic Acids Research，1988，16(14B)：7185-7186.
［6］Fukunaga K，Kawase M，Kato K. Structural variation in the Waxy gene and differentiation in foxtail millet［Setaria italica(L.)P. Beauv.］：implications for multiple origins of the Waxy phenotype［J］. Molecular Genetics and Genomics，2002，268(2)：214-222.
［7］Karper R. Inheritance of Waxy endosperm in sorghum［J］. Journal of Heredity，1933，24(6)：257-262.
［8］Pedersen J F，Bean S R，Graybosch R A，et al. Characterization of Waxy grain sorghum lines in relation to granule-bound starch synthase［J］. Euphytica，2005，144(1/2)：151-156.
［9］Hamblin M T，Fernandez M G S，Tuinstra M R，et al. Sequence variation at candidate loci in the starch metabolism pathway in sorghum：prospects for linkage disequilibrium mapping［J］. Crop Science，2007，47(Suppl)：125-134.
［10］Mcintyre C L，Drenth J，Gonzalez N，et al. Molecular characterization of the Waxy locus in sorghum［J］. Genome，2008，51(7)：524-533.
［11］Sattler S E，Singh J，Haas E J，et al. Two distinct Waxy alleles impact the granule-bound starch synthase in sorghum［J］. Molecular Breeding，2009，24(4)：349-359.
［12］Pedersen J F，Bean S R，Funnell D L，et al. Rapid iodine staining techniques for identifying the Waxy phenotype in sorghum grain and Waxy genotype in sorghum pollen［J］. Crop Science，2004，44：764-767.
［13］范明顺，张崇玉，张琴，等. 双波长分光光度法测定高粱中的直链淀粉和支链淀粉［J］. 中国酿造，2008(11)：85-87.
［14］Doyle J J，Doyle J L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue［J］. Phytochem Bull，1987，19：11-15.
［15］Sang Y J，Bean S，Seib P A，et al. Structure and functional properties of sorghum starches differing in amylose content［J］. Journal of Agricultural and Food Chemistry，2008，56(15)：6680-6685.
［16］Han X Z，Campanella O H，Guan H，et al. Influence of maize starch granule-associated protein on the rheological properties of starch pastes.Part Ⅱ. Dynamic measurements of viscoelastic properties of starch pastes［J］. Carbohydrate Polymers，2002，49(3)：323-330.
［17］Pedersen J F，Graybosch R A，Funnell D L. Occurrence of the Waxy alleles wxa and wxb in Waxy sorghum plant introductions and their effect on starch thermal properties［J］. Crop Science，2007，47：1927-1933.
［18］Rooney L W，Pflugfelder R L. Factors affecting starch digestibility with special emphasis on sorghum and corn［J］. Journal of Animal Science，1986，63(5)：1607-1623.
［19］Wong J H，Lau T，Cai N，et al. Digestibility of protein and starch from sorghum(Sorghum bicolor)is linked to biochemical and structural features of grain endosperm［J］. Journal of Cereal Science，2009，49(1)：73-82.

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备注/Memo

备注/Memo:
收稿日期：2013-09-23
基金项目：国家农业科技成果转化资金(编号：2013GB2F200437)；农业部引进的国际先进农业科学技术项目(编号：2012-Z37)；贵州省国际科技合作计划(编号：黔科合外G字［2012］7014)；贵州省科技计划［编号：黔科合NYC(2013)3016］。
作者简介：李祥栋(1987—)，男，山东临沂人，硕士研究生，研究方向为植物生理与分子调控。
通信作者：张明生，教授，博士生导师。E-mail：mshzhang@163.com。
更新日期/Last Update: 2014-05-25