[1]徐璐,汪涛,邹庆军,等.海雀稗响应干旱胁迫的生理与转录组分析[J].江苏农业科学,2025,53(22):185-192.
 Xu Lu,et al.Physiological response and transcriptome analysis of seashore paspalum (Paspalum vaginatum) under drought stress[J].Jiangsu Agricultural Sciences,2025,53(22):185-192.
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海雀稗响应干旱胁迫的生理与转录组分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第22期
页码:
185-192
栏目:
园艺与林学
出版日期:
2025-11-20

文章信息/Info

Title:
Physiological response and transcriptome analysis of seashore paspalum (Paspalum vaginatum) under drought stress
作者:
徐璐1汪涛2邹庆军2李成忠1
1.江苏农牧科技职业学院,江苏泰州 225300; 2.南京农业大学,江苏南京 210095
Author(s):
Xu Luet al
关键词:
海雀稗干旱胁迫生理响应转录组分析
Keywords:
-
分类号:
S184;S688.401
DOI:
-
文献标志码:
A
摘要:
为了探究海雀稗响应干旱胁迫的生理和分子机制,以海雀稗Salam为试验材料,设置正常灌溉(对照组)和自然干旱胁迫(试验组)2个处理,对持续干旱处理4、8、12、16 d的海雀稗进行生理指标的测定,对干旱胁迫12 d的海雀稗进行转录组测序。结果表明,在持续干旱胁迫下,海雀稗的叶片相对含水量呈下降趋势,从胁迫后8 d起显著低于对照。持续干旱胁迫4~16 d期间,海雀稗植株叶片的抗氧化酶[超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)]活性,可溶性糖、总蛋白、脯氨酸含量均显著高于对照,且都表现为先上升后下降的趋势;丙二醛含量在持续干旱胁迫4~16 d期间逐渐升高,在胁迫16 d时含量达到最大值,且均显著高于对照。通过转录组分析共获得7 065个差异表达基因(DEG),其中上调表达基因2 660个,占比为37.65%,下调表达基因4 405个,占比62.35%。差异表达基因在20条KEGG代谢通路上得到显著富集。其中,光合作用-天线蛋白、光合作用、苯丙烷生物合成、植物激素信号转导、光合生物的固碳作用、淀粉和蔗糖代谢等代谢途径富集到的差异表达基因最多,与海雀稗的耐旱性密切相关。综上所述,研究从生理、转录水平探究了海雀稗对干旱胁迫的响应,为海雀稗耐旱基因的挖掘及耐旱新品种的培养提供了一定的理论依据。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2024-09-30
基金项目:江苏农牧科技职业学院校级科研项目(编号:NSF2024ZR10);江苏省林业科技示范推广项目[编号:LYKJ(2020)29];泰州市中药材种质资源综合利用工程研究中心基金[编号:泰发改发(2023)133号]。
作者简介:徐璐(1989—),女,江苏扬州人,硕士,讲师,主要从事观赏植物栽培生理生态及应用等研究工作。E-mail:775011253@qq.com。
通信作者:李成忠,博士,教授,主要从事观赏植物栽培生理生态及应用等研究工作。E-mail:lichengzhong@126.com。
更新日期/Last Update: 2025-11-20