[1]刘文雍,吕梦阳,马琪,等.非生物胁迫下植物γ氨基丁酸调控机制研究进展[J].江苏农业科学,2026,54(8):29-37.
 Liu Wenyong,et al.Research progress of plant γ aminobutyric acid (GABA) regulation mechanism under abiotic stress[J].Jiangsu Agricultural Sciences,2026,54(8):29-37.
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非生物胁迫下植物γ氨基丁酸调控机制研究进展()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第8期
页码:
29-37
栏目:
专论与综述
出版日期:
2026-04-20

文章信息/Info

Title:
Research progress of plant γ aminobutyric acid (GABA) regulation mechanism under abiotic stress
作者:
刘文雍吕梦阳马琪温彩霞刘瑷华傅俐源向兴节王兰兰
沈阳师范大学生命科学学院,辽宁沈阳110034
Author(s):
Liu Wenyonget al
关键词:
γ-氨基丁酸非生物胁迫植物抗逆性代谢调控信号转导
Keywords:
-
分类号:
S184
DOI:
-
文献标志码:
A
摘要:
γ-氨基丁酸(GABA)是一种广泛分布于生物体内的非蛋白氨基酸,在动物神经系统中作为关键信号分子发挥重要作用。近年研究发现,GABA在植物中同样承担重要的调控功能,尤其在非生物胁迫响应中表现出显著的生理调控作用。植物在生长过程中常面临干旱、洪涝、盐碱、极端温度及重金属等胁迫环境,GABA可通过多重协同机制增强植物抗逆性,包括:(1)激活抗氧化防御系统;(2)调节渗透平衡;(3)动态平衡碳氮代谢;(4)参与Ca2+/pH值/ROS信号串联及植物激素(如脱落酸、乙烯)网络调控,形成多层次胁迫响应体系。本文系统综述了非生物胁迫下植物GABA代谢的核心途径及其分子调控机制,重点解析了谷氨酸脱羧酶(GAD)、GABA转氨酶(GABA-T)及琥珀酸半醛脱氢酶(SSADH)等关键酶的动态活性调控、胁迫特异性信号通路的触发模式,以及GABA通过表观遗传修饰介导的长期适应性。基于现有研究,未来可聚焦下游信号转导的分子机制、基因编辑技术以及高效外源GABA的高效施用方法,以促进农业高质量发展。
Abstract:
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参考文献/References:

[1]Zhang H M,Zhu J H,Gong Z Z,et al. Abiotic stress responses in plants[J]. Nature Reviews Genetics,2022,23(2):104-119.
[2]Abdullah,Wani K I,Naeem M,et al. From neurotransmitter to plant protector:the intricate world of GABA signaling and its diverse functions in stress mitigation[J]. Journal of Plant Growth Regulation,2025,44(2):403-418.
[3]Islam S N U,Kouser S,Hassan P,et al. Gamma-aminobutyric acid interactions with phytohormones and its role in modulating abiotic and biotic stress in plants[J]. Stress Biology,2024,4(1):36.
[4]Jiang K Y,Yin Z,Zhou P,et al. The scavenging capacity of γ-aminobutyric acid for acrolein and the cytotoxicity of the formed adduct[J]. Food & Function,2020,11(9):7736-7747.
[5]叶妞. 低O2/高CO2气调调控核桃果实青皮GABA代谢和褐变的机制研究[D]. 杨凌:西北农林科技大学,2024:7-9.
[6]Milon R B,Hu P C,Zhang X Q,et al. Recent advances in the biosynthesis and industrial biotechnology of gamma-amino butyric acid[J]. Bioresources and Bioprocessing,2024,11(1):32.
[7]Hu Y X,Huang X,Xiao Q L,et al. Advances in plant GABA research:biological functions,synthesis mechanisms and regulatory pathways[J]. Plants,2024,13(20):2891.
[8]Gupta D K,Corpas F J. Hormones and plant response[M]. Cham:Springer International Publishing,2021:291-314.
[9]Hou Y Y,Deng R,Shataer D,et al. L-Glutamate treatment alleviates chilling injury of prune (Prunus domestica L.) fruit by regulating ROS homeostasis,GABA shunt,and energy metabolism[J]. Food Chemistry,2024,461:140899.
[10]Zhang Q L,Zhu L,Li H L,et al. Insights and progress on the biosynthesis,metabolism,and physiological functions of gamma-aminobutyric acid (GABA):a review[J]. PeerJ,2024,12:e18712.
[11]Li Y X,Liu C L,Sun X,et al. Overexpression of MdATG18a enhances alkaline tolerance and GABA shunt in apple through increased autophagy under alkaline conditions[J]. Tree Physiology,2020,40(11):1509-1519.
[12]Liao J R,Wu X Y,Xing Z Q,et al. γ-aminobutyric acid (GABA) accumulation in tea (Camellia sinensis L.) through the GABA shunt and polyamine degradation pathways under anoxia[J]. Journal of Agricultural and Food Chemistry,2017,65(14):3013-3018.
[13]Fait A,Fromm H,Walter D,et al. Highway or byway:the metabolic role of the GABA shunt in plants[J]. Trends in Plant Science,2008,13(1):14-19.
[14]Astegno A,Capitani G,Dominici P. Functional roles of the hexamer organization of plant glutamate decarboxylase[J]. Biochimica et Biophysica Act-Proteins and Proteomics,2015,1854(9):1229-1237.
[15]Gout E,Bligny R,Douce R. Regulation of intracellular pH values in higher plant cells.Carbon-13 and phosphorus-31 nuclear magnetic resonance studies[J]. Journal of Biological Chemistry,1992,267(20):13903-13909.
[16]Bown A W,MacGregor K B,Shelp B J. Gamma-aminobutyrate:defense against invertebrate pests [J]. Trends in Plant Science,2006,11(9):424-427.
[17]Cholewa E,Bown A W,Cholewinski A J,et al. Cold-shock-stimulated γ-aminobutyric acid synthesis is mediated by an increase in cytosolic Ca2+,not by an increase in cytosolic H+[J]. Canadian Journal of Botany,1997,75(3):375-382.
[18]Zhang Z W,Dang T T,Yang X Y,et al. γ-aminobutyric acid alleviates programmed cell death in two Brassica species under cadmium stress[J]. International Journal of Molecular Sciences,2025,26(1):129.
[19]Renault H,Roussel V,El Amrani A,et al. The Arabidopsis pop2-1 mutant reveals the involvement of GABA transaminase in salt stress tolerance[J]. BMC Plant Biology,2010,10(1):20.
[20]Michaeli S,Fait A,Lagor K,et al. A mitochondrial GABA permease connects the GABA shunt and the TCA cycle,and is essential for normal carbon metabolism[J]. The Plant Journal,2011,67(3):485-498.
[21]Kim Y G,Lee S,Kwon O S,et al. Redox-switch modulation of human SSADH by dynamic catalytic loop[J]. The EMBO Journal,2009,28(7):959-968.
[22]Shelp B J,Bown A W,McLean M D. Metabolism and functions of gamma-aminobutyric acid[J]. Trends in Plant Science,1999,4(11):446-452.
[23]Blázquez M A. Polyamines:their role in plant development and stress[J]. Annual Review of Plant Biology,2024,75(1):95-117.
[24]Roy T,Pal N,Das N. Regulation of the polyamine pool in plants:metabolic implications and stress mitigation,with emphasis on microbial influence[J]. Physiological and Molecular Plant Pathology,2024,132:102317.
[25]Dunn M F,Becerra-Rivera V A. The biosynthesis and functions of polyamines in the interaction of plant growth-promoting rhizobacteria with plants[J]. Plants,2023,12(14):2671.
[26]周沫霖. 低温和二氧化碳胁迫下龙眼γ-氨基丁酸富集与机理研究[D]. 广州:华南农业大学,2017:11-13.
[27]Signorelli S,Dans P D,Coitio E L,et al. Connecting proline and γ-aminobutyric acid in stressed plants through non-enzymatic reactions[J]. PLoS One,2015,10(3):e0115349.
[28]Matsuyama A,Yoshimura K,Shimizu C,et al. Characterization of glutamate decarboxylase mediating γ-amino butyric acid increase in the early germination stage of soybean (Glycine max [L.]Merr)[J]. Journal of Bioscience and Bioengineering,2009,107(5):538-543.
[29]Xu J G,Hu Q P,Duan J L,et al. Dynamic changes in γ-aminobutyric acid and glutamate decarboxylase activity in oats (Avena nuda L.) during steeping and germination[J]. Journal of Agricultural and Food Chemistry,2010,58(17):9759-9763.
[30]Sharma S,Saxena D C,Riar C S. Analysing the effect of germination on phenolics,dietary fibres,minerals and γ-amino butyric acid contents of barnyard millet (Echinochloa frumentaceae)[J]. Food Bioscience,2016,13:60-68.
[31]Xu L,Chen L,Ali B,et al. Impact of germination on nutritional and physicochemical properties of adlay seed (Coix lachryma-jobi L.)[J]. Food Chemistry,2017,229:312-318.
[32]Zhao G C,Xie M X,Wang Y C,et al. Molecular mechanisms underlying γ-aminobutyric acid (GABA) accumulation in giant embryo rice seeds[J]. Journal of Agricultural and Food Chemistry,2017,65(24):4883-4889.
[33]Kim M J,Kwak H S,Kim S S. Effects of germination on protein,γ-aminobutyric acid,phenolic acids,and antioxidant capacity in wheat[J]. Molecules,2018,23(9):2244.
[34]Al-Quraan N A,Al-Ajlouni Z I,Obedat D I. The GABA shunt pathway in germinating seeds of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) under salt stress[J]. Seed Science Research,2019,29(4):250-260.
[35]Sheng Y D,Xiao H Y,Guo C L,et al. Effects of exogenous gamma-aminobutyric acid on α-amylase activity in the aleurone of barley seeds[J]. Plant Physiology and Biochemistry,2018,127:39-46.
[36]Baum G,Lev-Yadun S,Fridmann Y,et al. Calmodulin binding to glutamate decarboxylase is required for regulation of glutamate and GABA metabolism and normal development in plants[J]. The EMBO Journal,1996,15(12):2988-2996.
[37]Kathiresan A,Miranda J,Chinnappa C C,et al. γ-aminobutyric acid promotes stem elongation in Stellaria longipes:the role of ethylene[J]. Plant Growth Regulation,1998,26(2):131-137.
[38]宫雅昕. γ-氨基丁酸代谢调控拟南芥叶片发育的分子机制研究[D]. 武汉:中南民族大学,2020:14-17.
[39]Ma H. Plant reproduction:GABA gradient,guidance and growth[J]. Current Biology,2003,13(21):R834-R836.
[40]Palanivelu R,Brass L,Edlund A F,et al. Pollen tube growth and guidance is regulated by POP2 an Arabidopsis gene that controls GABA levels[J]. Cell,2003,114(1):47-59.
[41]Yang Z B. GABA,a new player in the plant mating game[J]. Developmental Cell,2003,5(2):185-186.
[42]Renault H,El Amrani A,Palanivelu R,et al. GABA accumulation causes cell elongation defects and a decrease in expression of genes encoding secreted and cell wall-related proteins in Arabidopsis thaliana[J]. Plant & Cell Physiology,2011,52(5):894-908.
[43]Takayama M,Ezura H. How and why does tomato accumulate a large amount of GABA in the fruit [J]. Frontiers in Plant Science,2015,6:612.
[44]Nonaka S,Arai C,Takayama M,et al. Efficient increase of γ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis[J]. Scientific Reports,2017,7:7057.
[45]张海龙,陈迎迎,杨立新,等. γ-氨基丁酸对植物生长发育和抗逆性的调节作用[J]. 植物生理学报,2020,56(4):600-612.
[46]Wang J R,Zhang Y,Wang J Z,et al. Promoting γ-aminobutyric acid accumulation to enhances saline-alkali tolerance in tomato[J]. Plant Physiology,2024,196(3):2089-2104.
[47]Zhao Q Y,Ma Y,Huang X Q,et al. GABA application enhances drought stress tolerance in wheat seedlings (Triticum aestivum L.) [J]. Plants,2023,12(13):2495.
[48]Su N N,Wu Q,Chen J H,et al. GABA operates upstream of H+-ATPase and improves salinity tolerance in Arabidopsis by enabling cytosolic K+ retention and Na+ exclusion[J]. Journal of Experimental Botany,2019,70(21):6349-6361.
[49] Rajani M S,Bedair M F,Li H,et al. Phenotypic effects from the expression of a deregulated AtGAD1 transgene and GABA pathway suppression mutants in maize [J]. PLoS One,2021,16(12):e0259365.
[50]Di Y L,Cao Y Q,Peng D D,et al. AsGAD1 cloned from creeping bentgrass modulates cadmium tolerance of Arabidopsis thaliana by remodelling membrane lipids and cadmium uptake,transport and chelation [J]. Physiologia Plantarum,2025,177(1): e70063.
[51]Wu X L,Jia Q Y,Ji S X,et al. Gamma-aminobutyric acid (GABA) alleviates salt damage in tomato by modulating Na+ uptake,the GAD gene,amino acid synthesis and reactive oxygen species metabolism[J]. BMC Plant Biology,2020,20(1):465.
[52]Guo Z X,Lv J L,Dong X X,et al. Gamma-aminobutyric acid improves phenanthrene phytotoxicity tolerance in cucumber through the glutathione-dependent system of antioxidant defense[J]. Ecotoxicology and Environmental Safety,2021,217:112254.
[53]Hao X H,Liu K X,Zhang M Y. Effect of exogenous γ-aminobutyric acid on physiological property,antioxidant activity,and cadmium uptake of quinoa seedlings under cadmium stress[J]. Bioscience Reports,2024,44(6):BSR20240215.
[54]Stitt M. Nitrate regulation of metabolism and growth[J]. Current Opinion in Plant Biology,1999,2(3):178-186.
[55]Breitkreuz K E,Shelp B J,Fischer W N,et al. Identification and characterization of GABA,proline and quaternary ammonium compound transporters from Arabidopsis thaliana[J]. FEBS Letters,1999,450(3):280-284.
[56]Sulieman S,Schulze J. Phloem-derived γ-aminobutyric acid (GABA) is involved in upregulating nodule N2 fixation efficiency in the model legume Medicago truncatula[J]. Plant,Cell & Environment,2010,33(12):2162-2172.
[57]Renault H,EL Amrani A,Berger A,et al. γ-aminobutyric acid transaminase deficiency impairs central carbon metabolism and leads to cell wall defects during salt stress in Arabidopsis roots[J]. Plant,Cell & Environment,2013,36(5):1009-1018.
[58]Michaeli S,Fromm H. Closing the loop on the GABA shunt in plants:are GABA metabolism and signaling entwined [J]. Frontiers in Plant Science,2015,6:419.
[59]Chen W,Meng C,Ji J,et al. Exogenous GABA promotes adaptation and growth by altering the carbon and nitrogen metabolic flux in poplar seedlings under low nitrogen conditions[J]. Tree Physiology,2020,40(12):1744-1761.
[60]Shelp B J,Bozzo G G,Trobacher C P,et al. Hypothesis/review:contribution of putrescine to 4-aminobutyrate (GABA) production in response to abiotic stress[J]. Plant Science,2012,193:130-135.
[61]Seifikalhor M,Aliniaeifard S,Hassani B,et al. Diverse role of γ-aminobutyric acid in dynamic plant cell responses[J]. Plant Cell Reports,2019,38(8):847-867.
[62]Li Z,Yu J J,Peng Y,et al. Metabolic pathways regulated by γ-aminobutyric acid (GABA) contributing to heat tolerance in creeping bentgrass (Agrostis stolonifera)[J]. Scientific Reports,2016,6:30338.
[63]Kudla J,Becker D,Grill E,et al. Advances and current challenges in calcium signaling[J]. New Phytologist,2018,218(2):414-431.
[64]Zhu J K. Abiotic stress signaling and responses in plants[J]. Cell,2016,167(2):313-324.
[65]Bown A W,Shelp B J. The metabolism and functions of γ-aminobutyric acid[J]. Plant Physiology,1997,115(1):1-5.
[66]Mittler R,Vanderauwera S,Suzuki N,et al. ROS signaling:the new wave [J]. Trends in Plant Science,2011,16(6):300-309.
[67]Kim J M,To T K,Seki M. An epigenetic integrator:new insights into genome regulation,environmental stress responses and developmental controls by histone deacetylase 6[J]. Plant and Cell Physiology,2012,53(5):794-800.
[68]Bouché N,Fait A,Bouchez D,et al. Mitochondrial succinic-semialdehyde dehydrogenase of the γ-aminobutyrate shunt is required to restrict levels of reactive oxygen intermediates in plants[J]. Proceedings of the National Academy of Sciences of the United States of America,2003,100(11):6843-6848.
[69]Toyota M,Spencer D,Sawai-Toyota S,et al. Glutamate triggers long-distance,calcium-based plant defense signaling[J]. Science,2018,361(6407):1112-1115.
[70]Rocha M,Licausi F,Araújo W L,et al. Glycolysis and the tricarboxylic acid cycle are linked by alanine aminotransferase during hypoxia induced by waterlogging of Lotus japonicus[J]. Plant Physiology,2010,152(3):1501-1513.
[71]Verslues P E,Sharma S. Proline metabolism and its implications for plant-environment interaction[J]. The Arabidopsis Book,2010,8:e0140.
[72]Wu C J,Yuan D Y,Liu Z Z,et al. Conserved and plant-specific histone acetyltransferase complexes cooperate to regulate gene transcription and plant development[J]. Nature Plants,2023,9(3):442-459.
[73]Budhagatapalli N,Hensel G. Multiplexed genome editing in plants using CRISPR/cas-based endonuclease systems[M]//Genome editing. Cham:Springer International Publishing,2022:143-169.

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备注/Memo

备注/Memo:
收稿日期:2025-08-12
基金项目:辽宁省基本科研业务费支持项目-重大项目孵化工程专项(编号:LJ202410166051);沈阳师范大学创新训练项目(编号:202506020)。
作者简介:刘文雍(1999—),男,甘肃陇南人,硕士研究生,主要从事环境变化下植物生理生态响应研究。E-mail:1114048796@qq.com。
通信作者:王兰兰,博士,教授,主要从事环境变化下植物生理生态响应研究。E-mail:wangqi5387402006@163.com。
更新日期/Last Update: 2026-04-20