|本期目录/Table of Contents|

[1]王桃云,邱业先,汪金莲,等.银杏叶抑制脲酶活性成分提取条件优化及其成分初步鉴定[J].江苏农业科学,2013,41(10):246-249.
 Wang Taoyun,et al.Optimization of extraction conditions and preliminary identification of inhibitory active ingredients to urease from Ginkgo biloba leaves[J].Jiangsu Agricultural Sciences,2013,41(10):246-249.
点击复制

银杏叶抑制脲酶活性成分提取条件优化
及其成分初步鉴定
(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年10期
页码:
246-249
栏目:
贮藏与加工
出版日期:
2013-10-25

文章信息/Info

Title:
Optimization of extraction conditions and preliminary identification of inhibitory active ingredients to urease from Ginkgo biloba leaves
作者:
王桃云邱业先汪金莲赵琪盛齐王罗玲
苏州科技学院化学与生物工程学院,江苏苏州 215009
Author(s):
Wang Taoyunet al
关键词:
银杏叶抑制脲酶成分正交试验
Keywords:
-
分类号:
R284.2
DOI:
-
文献标志码:
A
摘要:
以脲酶活性抑制率为评价指标,采用单因素试验和正交试验对银杏叶抑制脲酶活性成分的提取条件进行优化;并应用化学反应鉴定法,对银杏叶抑制脲酶活性成分进行了初步鉴定。结果表明:银杏叶抑制脲酶活性成分的最佳提取工艺为微波功率231 W、液料比20 mL ∶[KG-*3]1 g、提取时间2 min、乙醇体积分数60%,该工艺条件下银杏叶提取液对脲酶活性的抑制率达55.79%;银杏叶中抑制脲酶活性的主要成分为多酚及黄酮类化合物。
Abstract:
-

参考文献/References:

[1]赵略,孙庆元,于英梅,等. 脲酶抑制剂nBPT对土壤脲酶活性和脲酶产生菌的影响[J]. 大连轻工业学院学报,2007,26(1):24-27.
[2]武慧玲,薛立微,王胜华,等. 小叶女贞叶提取物对脲酶的抑制作用[J]. 土壤通报,2011,42(2):311-315.
[3]倪秀菊,李玉中,徐春英,等. 土壤脲酶抑制剂和硝化抑制剂的研究进展[J]. 中国农学通报,2009,25(12):145-149.
[4]Dawar K,Zaman M,Rowarth J S,et al.Urease inhibitor reduces N losses and improves plant-bioavailability of urea applied in fine particle and granular forms under field conditions[J]. Agriculture,Ecosystems and Environment,2011,144(1):41-50.
[5]Ariz I,Cruchaga S,Lasa B,et al.The physiological implications of urease inhibitors on N metabolism during germination of Pisum sativum and Spinacea oleracea seeds[J]. Journal of Plant Physiology,2012,169(7):673-681.
[6]邵胜荣. 银杏叶活性成分提取纯化、制剂工艺及质量研究[D]. 杭州:浙江大学,2005:1-80.
[7]Eivazi F,Bayan M R,Schmidt K.Select soil enzyme activities in the historic Sanborn field as affected by long-term cropping system[J]. Communications in Soil Science and Plant Analysis,2003,34(15/16):2259-2275.
[8]Chen Y,Xie M Y,Gong X F,et al.Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum[J]. Journal of Food Engineering,2007,81(1):162-170.
[9]Sun Y X,Li Y J,Li M Q,et al.Optimization of extraction technology of the Anemone raddeana polysaccharides(ARP)by orthogonal test design and evaluation of its anti-tumor activity[J]. Carbohydrate Polymers,2009,75(4):575-579.
[10]张艳军,彭重威,徐淑庆,等. 银叶树树叶中总黄酮提取工艺优化[J]. 中药材,2012,35(4):638-641.
[11]谢普军,黄立新,张彩虹,等. 低温减压沸腾提取橄榄苦苷的工艺研究[J]. 中国中药杂志,2012,37(13):1946-1951.
[12]梁臣艳,甄汉深,黄晓玉,等. 广西容县柚子皮化学鉴别的初步研究[J]. 中国民族民间医药,2010,19(5):35-36.
[13]汪金莲,邱业先,陈宏伟,等. 茶多酚对几种植物病原真菌的抑制作用[J]. 江苏农业科学,2007(4):61-63.
[14]黄赛鸳,赵志东,伍文辉,等. 黄酮类化合物对产脲酶真菌生长的影响[J]. 仲恺农业工程学院学报,2009,22(1):13-16.

相似文献/References:

[1]刘晓燕,谢国芳,秦晋颖,等.不同抗氧化剂对油炸马铃薯片品质的影响[J].江苏农业科学,2013,41(11):289.
 Liu Xiaoyan,et al.Effect of different antioxidants on quality of fried potato chips[J].Jiangsu Agricultural Sciences,2013,41(10):289.
[2]焦铭,李笔,孙雪,等.双波长分光光度法测定银杏叶中直链淀粉、支链淀粉含量[J].江苏农业科学,2014,42(04):275.
 Jiao Ming,et al.Determination of amylose and amylopectin in ginkgo leaves by dual wavelength spectrophotometry[J].Jiangsu Agricultural Sciences,2014,42(10):275.
[3]杨振东,蒋彦婕.金银花、银杏叶复合饮料的研制[J].江苏农业科学,2015,43(12):303.
 Yang Zhendong,et al.Study on compound beverage of honeysuckle and ginkgo leaf[J].Jiangsu Agricultural Sciences,2015,43(10):303.
[4]蒲擎宇,杨朗生,王 刚.银杏叶原料与产品中游离黄酮苷元含量的相关性分析[J].江苏农业科学,2015,43(07):344.
 Pu Qingyu,et al.Correlation analysis of free flavonoid aglycone contents in ginkgo leaves and its extracts[J].Jiangsu Agricultural Sciences,2015,43(10):344.
[5]李楠,许文凤,叶振南,等.9种植物粗提总黄酮的体外降脂及抑制非酶糖基化的活性[J].江苏农业科学,2014,42(06):291.
 Li Nan,et al.Study on hypolipemic and inhibition of nonenzymatic glycosylation activity in vitro of crude flavone form nine plants[J].Jiangsu Agricultural Sciences,2014,42(10):291.
[6]王莹,王华,丁宁,等.响应曲面法优化水溶性银杏叶多糖提取工艺[J].江苏农业科学,2015,43(05):268.
 Wang Ying,et al.Extraction technology of water-soluble polysaccharides from Ginkgo biloba leaves by response surface methodology[J].Jiangsu Agricultural Sciences,2015,43(10):268.
[7]陆艳凤,周明夏,孙国波.不同剂量女贞、银杏叶提取物对苏北草鸡生产性能的影响[J].江苏农业科学,2014,42(07):207.
 Lu Yanfeng,et al.Effects of different doses of glossy privet fruit and ginkgo biloba extract on production performance of northern Jiangsu chicken[J].Jiangsu Agricultural Sciences,2014,42(10):207.
[8]林标声,吴江文,江火香,等.适合银杏叶发酵的菌株筛选及发酵效果[J].江苏农业科学,2016,44(04):315.
 Lin Biaosheng,et al.Screening of strains suitable for ginkgo leaf fermentation and its fermentation effect[J].Jiangsu Agricultural Sciences,2016,44(10):315.
[9]曲威,孙丽英,江晨洁,等.鸡场沼液对农作物病原真菌的抑制作用[J].江苏农业科学,2018,46(16):83.
 Qu Wei,et al.Inhibiting effect of biogas slurry on plant pathogenic fungi[J].Jiangsu Agricultural Sciences,2018,46(10):83.

备注/Memo

备注/Memo:
收稿日期:2013-03-26
基金项目:江苏省科技创新与成果转化(重大科技支撑与自主创新)专项资金(编号:BE2010388)。
作者简介:王桃云(1973—),男,江西安福人,博士,副教授,主要从事植物资源与天然产物研究与开发。E-mail:wangtaoyun@mail.usts.edu.cn。
通信作者:邱业先(1954—),男,江西永丰人,教授,主要从事蛋白质、酶研究及天然产物研究与开发。E-mail:qyx542@mail.usts.edu.cn。
更新日期/Last Update: 2013-10-25