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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第42卷

期数:

2014年02期

页码:

155-158

栏目:

畜牧兽医

出版日期:

2014-02-25

文章信息/Info

Title:

Protective effect of sodium selenite on oxidative damage of mammary gland epithelial cells caused by hydrogen peroxide in Yanbian dairy goat

作者:

孙婧陶¹;  刘佳丽²;  张宝修¹;  茹李波¹;  李钟淑¹;  方南洙¹

1.延边大学农学院/动物遗传育种与繁殖实验室，吉林延吉 133002； 2.吉林省安图县畜牧兽医局，吉林安图 133600

Author(s):

Sun Jingtao; et al

关键词:

亚硒酸钠; 乳腺上皮细胞; 氧化损伤; 凋亡

Keywords:

-

分类号:

S827.1

DOI:

-

文献标志码:

A

摘要:

研究了亚硒酸钠对过氧化氢导致的延边奶山羊乳腺上皮细胞氧化损伤的保护作用。将延边奶山羊乳腺上皮细胞分为3组：正常组(对照组1)、过氧化氢损伤组(对照组2)、亚硒酸钠保护组。MTT方法检测细胞活力，Hoechst33342/PI 双染、Giemsa染色法检测细胞凋亡情况，DHE染色法检测细胞内ROS含量。结果表明，与对照组2相比，浓度为0.25、0.5、1.0 μg/mL的亚硒酸钠保护组的延边奶山羊乳腺上皮细胞活力上升，细胞凋亡数目减少，活性氧含量减少。其中以0.5 μg/mL亚硒酸钠处理组效果最显著。说明0.5 μg/mL的亚硒酸钠可以减少过氧化氢所致的延边奶山羊乳腺上皮细胞的氧化损伤，提高细胞活力，抑制细胞凋亡，减少细胞内ROS含量。表明亚硒酸钠对延边奶山羊乳腺上皮细胞过氧化氢所致氧化损伤具有保护作用。

Abstract:

-

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备注/Memo

备注/Memo:

收稿日期：2013-07-23
基金项目：吉林省科技厅重点项目(编号：20100228)。
作者简介：孙婧陶(1990—)，女，河北承德人，硕士，主要从事动物繁殖与生物技术研究。E-mail：sunjt1990@126.com。
通信作者：方南洙(1960—)，男，朝鲜族，博士，教授，主要从事动物繁殖与生物技术研究。E-mail：nzfang@ybu.edu.cn。

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更新日期/Last Update: 2014-02-25