|本期目录/Table of Contents|

[1]崔东亚,杨美玲.降解组测序与剪切位点分析研究进展[J].江苏农业科学,2014,42(07):56-60.
　Cui Dongya,et al.Research progress of degradome sequencing and splice site analysis[J].Jiangsu Agricultural Sciences,2014,42(07):56-60.
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降解组测序与剪切位点分析研究进展(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年07期
页码:
56-60
栏目:
生物技术
出版日期:
2014-07-25

文章信息/Info

Title:
Research progress of degradome sequencing and splice site analysis
作者:
崔东亚1;  杨美玲2
1.运城学院生命科学系，山西运城 044000； 2.运城学院应用化学系，山西运城 044000
Author(s):
Cui Dongya; et al
关键词:
降解组; miRNA; 靶基因; 剪切位点; 研究进展
Keywords:
-
分类号:
Q754
DOI:
-
文献标志码:
A
摘要:
动植物细胞内都存在大量的microRNA(miRNA)，miRNA的功能之一是通过互补指导内切酶切割与之互补的mRNA，对该mRNA进行转录后调控。但miRNA与靶基因之间并不是完全互补，所以通过序列计算方式预测的靶基因中假阳性也是很高的。单独验证预测的靶基因不能确定是否正确且费时费力，高通量测序与计算预测结合可以很好地验证和发现miRNA的靶基因。介绍了寻找miRNA靶基因的降解组测序方法，包括降解组测序的方法原理、试验流程、miRNA靶基因寻找等主要环节。经研究发现，降解组测序已经成为寻找miRNA靶基因的常用方法。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2013-10-26
作者简介：崔东亚(1978—)，男，河北满城人，硕士，讲师，主要从事分子生物教学与研究。E-mail：dongyacui@163.com。
更新日期/Last Update: 2014-07-25