|本期目录/Table of Contents|

[1]李慧,李爱,彭立新,等.杨树EBP1基因的克隆与表达载体构建[J].江苏农业科学,2015,43(04):14-17.
　Li Hui,et al.Cloning of EBP1 gene from populus and construction of its plant expression vector[J].Jiangsu Agricultural Sciences,2015,43(04):14-17.
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杨树EBP1基因的克隆与表达载体构建(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年04期
页码:
14-17
栏目:
生物技术
出版日期:
2015-04-25

文章信息/Info

Title:
Cloning of EBP1 gene from populus and construction of its plant expression vector
作者:
李慧;  李爱;  彭立新;  阎国荣
天津农学院园艺园林学院，天津 300384
Author(s):
Li Hui; et al
关键词:
杨树; EBP1基因; 克隆; 表达载体; 构建; 基因序列分析; 蛋白同源性比对
Keywords:
-
分类号:
Q943.2
DOI:
-
文献标志码:
A
摘要:
器官大小是植物形态的重要表现特征之一，也是决定一些作物或经济植物产量和品质的重要因素。研究证实，草本植物如拟南芥中EBP1类转录因子可从细胞数量和体积2个方面同时调控植物器官大小，但该基因在木本植物杨树中的结构及功能仍未知。本研究依托杨树全基因组及EST数据信息，采用生物信息学方法结合基因同源序列克隆，首次对杨树中的EBP1全长cDNA进行克隆及序列分析，同时成功构建了用于遗传转化的过表达载体。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2014-05-25
基金项目：天津市高等学校科技发展基金(编号：20120619)；天津市应用基础与前沿技术研究计划(编号：14JCQNJC15000)。
作者简介：李慧(1981—)，女，河北廊坊人，硕士，讲师，主要从事植物分子生物学研究。E-mail：lihui@tjau.edu.cn。
通信作者：阎国荣，博士，教授，主要从事植物资源学研究。E-mail：yanguorong@eyou.com。
更新日期/Last Update: 2015-04-25