|本期目录/Table of Contents|

[1]孙来玉,钱坤,杨金田,等.免疫芯片法追溯检测克伦特罗用药史研究[J].江苏农业科学,2015,43(05):275-279.
 Sun Laiyu,et al.Study on traceability of use of clenbuterol by immunochip analysis[J].Jiangsu Agricultural Sciences,2015,43(05):275-279.
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免疫芯片法追溯检测克伦特罗用药史研究(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年05期
页码:
275-279
栏目:
质量安全与检测分析
出版日期:
2015-05-25

文章信息/Info

Title:
Study on traceability of use of clenbuterol by immunochip analysis
作者:
孙来玉 钱坤 杨金田 邵朝纲 尹莉
湖州师范学院生命科学学院,浙江湖州 313000
Author(s):
Sun Laiyuet al
关键词:
免疫芯片克伦特罗高通量监测技术毛发尿液黑鬃白鬃
Keywords:
-
分类号:
TS201.6
DOI:
-
文献标志码:
A
摘要:
以雄性皖浙花猪毛发、尿液为检测材料,以免疫芯片为检测手段,考察克伦特罗残留规律。结果表明,免疫芯片法检测动物毛发样品的检测限和定量限分别为0.15、0.50 ng/g,回归方程为:y=-0.391x3+12.576x2-141.88x+867.84,r2=0.999 4,线性范围为0.5~16.0 ng/g,回收率为65.2%~81.4%,休药期42 d仍能检测出毛发中克伦特罗残留。免疫芯片法检测动物尿液样品的检测限和定量限分别为0.20、0.60 ng/mL,回归方程为:y=-0.107 1x3+6.179 5x2-110.33x+839.37,r2=0.992 3,线性范围为0.6~16.0 ng/mL,回收率为61.5%~72.2%,休药期7 d基本不能检测出克伦特罗残留。免疫芯片法检测动物毛发克伦特罗残留快速、简单、准确,适合规模化检测,可用于克伦特罗用药史追溯性检测。
Abstract:
-

参考文献/References:

[1]孙来玉,尹莉,姚婷,等. 盐酸克伦特罗残留检测研究进展[J]. 湖州师范学院学报,2014,36(4):27-32.
[2]Zhang Y F,Yue C J,Xie F. Fluorimetric method based on diazotization-coupling reaction for determination of clenbuterol[J]. Journal of Fluorescence,2014,24(3):945-950.
[3]González-Antua A,Rodríguez-González P,Lavandera I,et al. Development of a routine method for the simultaneous confirmation and determination of clenbuterol in urine by minimal labeling isotope pattern deconvolution and GC-EI-MS[J]. Analytical and Bioanalytical Chemistry,2012,402(5):1879-1888.
[4]Nielen M,Lasaroms J,Essers M L,et al. Multiresidue analysis of beta-agonists in bovine and porcine urine,feed and hair using liquid chromatography electrospray ionisation tandem mass spectrometry[J]. Analytical and Bioanalytical Chemistry,2008,391(1):199-210.
[5]Mitrevski B S,Wilairat P,Marriott P J. Evaluation of world anti-doping agency criteria for anabolic agent analysis by using comprehensive two-dimensional gas chromatography-mass spectrometry[J]. Analytical and Bioanalytical Chemistry,2010,396(7):2503-2511.
[6]Li Y,Hu J T,Shi Y,et al. Simultaneous determination of seven adulterants in slimming functional foods by HPLC-ESI-MS/MS[J]. Food Analytical Methods,2011,4(4):505-516.
[7]Zheng H,Deng L G,Lu X,et al. UPLC-ESI-MS-MS determination of three β2-agonists in pork[J]. Chromatographia,2010,72(1/2):79-84.
[8]Vela J,Yanes E G,Stalcup A M. Quantitative determination of clenbuterol,salbutamol and tulobuterol enantiomers by capillary electrophoresis[J]. Journal of Analytical Chemistry,2001,369(3/4):212-219.
[9]Jiao F,Chen X,Hu Y,et al. Enantiomeric separation of racemic clenbuterol by capillary zone electrophoresis[J]. Journal of the Iranian Chemical Society,2008,5(4):553-558.
[10]Zhang M Z,Wang M Z,Chen Z L,et al. Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of clenbuterol and ractopamine in swine urine[J]. Analytical and Bioanalytical Chemistry,2009,395(8):2591-2599.
[11]Liu X X,Wang H,Liang Y,et al. Production and characterization of a single-chain Fv antibody-alkaline phosphatase fusion protein specific for clenbuterol[J]. Molecular Biotechnology,2010,45(1):56-64.
[12]Zhong L,Zhang W,Zer C,et al. Protein microarray:sensitive and effective immunodetection for drug residues[J]. BMC Biotechnology,2010,10:12.
[13]孙来玉,尹莉,姚 婷,等. 瘦肉精克伦特罗残留检测免疫芯片技术的研究[J]. 药物分析杂志,2014,34(5):859-864.
[14]Reena R,Gaichore,Ashwini K,et al. Multiwalled carbon nanotube-4-tert-butyl calixarene composite electrochemical sensor for clenbuterol hydrochloride determination by means of differential pulse adsorptive stripping voltammetry[J]. Appl Electrochem,2012,42(9):979-987.
[15]Breault-Turcot J,Masson J F. Nanostructured substrates for portable and miniature SPR biosensors[J]. Analytical and Bioanalytical Chemistry,2012,403(6):1477-1484.
[16]Yang Y P,Lei X Y,Yue A,et al. Temperature-dependent THz vibrational spectra of clenbuterol hydrochloride[J]. Science China:Physics,Mechanics and Astronomy,2013,56(4):713-717.
[17]Srogi K. Testing for drugs in hair-a review of chromatographic procedures[J]. Microchimica Acta,2006,154(3/4):191-212.
[18]Zalko D,Perdu-Durand E,Debrauwer L,et al. Comparative metabolism of clenbuterol by rat and bovine liver microsomes and slices[J]. Drug Metabolism and Disposition:the Biological Fate of Chemicals,1998,26(1):28-35.
[19]Zalko D,Debrauwer L,Bories G,et al. Metabolism of clenbuterol in rats[J]. Drug Metabolism and Disposition,1998,26(9):891-899.

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备注/Memo

备注/Memo:
收稿日期:2015-01-20
基金项目:浙江省公益性应用研究计划(分析测试)(编号:2012C37027)。
作者简介:孙来玉(1974—),男,安徽无为人,实验师,研究方向为生物医药与食品安全。E-mail:sunly@hutc.zj.cn。
更新日期/Last Update: 2015-05-25