|本期目录/Table of Contents|

[1]吴孔阳,朱闪闪,和朝军,等.1株产3-羟基丙酸菌株的分离和鉴定[J].江苏农业科学,2015,43(09):381-383.
　Wu Kongyang,et al.Isolation and identification of a bacterial strain producing 3-hydroxypropionic acid[J].Jiangsu Agricultural Sciences,2015,43(09):381-383.
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1株产3-羟基丙酸菌株的分离和鉴定(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年09期
页码:
381-383
栏目:
资源与环境
出版日期:
2015-09-25

文章信息/Info

Title:
Isolation and identification of a bacterial strain producing 3-hydroxypropionic acid
作者:
吴孔阳1;  朱闪闪1;  和朝军1;  董文雅1;  杨同香2
1.洛阳师范学院生命科学学院，河南洛阳 471022； 2.河南科技大学食品与生物工程学院，河南洛阳 471023
Author(s):
Wu Kongyang; et al
关键词:
3-羟基丙酸; 分离; 鉴定; 短乳杆菌; 薄层层析
Keywords:
-
分类号:
Q939.9
DOI:
-
文献标志码:
A
摘要:
通过平板筛选，从土壤中分离出1株菌株Y31，并对该菌株进行形态观察、生理生化试验、16S rDNA序列分析以及产物的薄层层析鉴定。结果表明，Y31菌株初步鉴定为短乳杆菌变种，能够以丙酸为唯一碳源发酵生产3-羟基丙酸。
Abstract:
-

参考文献/References:

［1］Kumar V，Ashok S，Park S. Recent advances in biological production of 3-hydroxypropionic acid［J］. Biotechnology Advances，2013，31(6)：945-961.
［2］Lee S H，Park S J，Park O J，et al. Production of 3-hydroxypropionic acid from acrylic acid by newly isolated Rhodococcus erythropolis LG12［J］. Journal of Microbiology and Biotechnology，2009，19(5)：474-481.
［3］Thorgersen M P，Lipscomb G L，Schut G J，et al. Deletion of acetyl-CoA synthetases Ⅰ and Ⅱ increases production of 3-hydroxypropionate by the metabolically-engineered hyperthermophile Pyrococcus furiosus［J］. Metabolic Engineering，2014，22：83-88.
［4］Lee P，Subramanian M R，Zhou S F，et al. 3-Hydroxyisobutyrate dehydrogenase-Ⅰ from Pseudomonas denitrificans ATCC 13867 degrades 3-hydroxypropionic acid［J］. Biotechnology and Bioprocess Engineering，2014，19：1-7.
［5］Kim K，Kim S K，Park Y C，et al. Enhanced production of 3-hydroxypropionic acid from glycerol by modulation of glycerol metabolism in recombinant Escherichia coli［J］. Bioresource Technology，2014，156：170-175.
［6］Jung W S，Kang J H，Chu H S，et al. Elevated production of 3-hydroxypropionic acid by metabolic engineering of the glycerol metabolism in Escherichia coli［J］. Metabolic Engineering，2014，23：116-122.
［7］Dishisha T. Microbial production of bio-based chemicals［D］. Lund：Lund University，2013.
［8］Arasu M V，Sarkar R，Sekar B S，et al. Isolation of a novel Pseudomonas species SP2 producing vitamin B12 under aerobic condition［J］. Biotechnology and Bioprocess Engineering，2013，18(1)：43-51.
［9］范俊英，方慧英，诸葛斌，等. 3-羟基丙酸高产菌株的筛选及诱变选育［J］. 微生物学通报，2012，39(9)：1355-1362.
［10］Wang X，Sa N，Wang F H，et al. Engineered constitutive pathway in klebsiella pneumoniae for 3-Hydroxypropionic acid production and implications for decoupling glycerol dissimilation pathways［J］. Current Microbiology，2013，66(3)：293-299.
［11］周德庆. 微生物学实验教程［M］. 北京：高等教育出版社，2006.
［12］杜连祥，路福平.微生物学实验技术［M］. 北京：中国轻工业出版社，2005.
［13］奥斯伯F M，布伦特R，金斯顿R E，等. 精编分子生物学实验指南［M］. 5版. 北京：科学出版社，2008.
［14］李冰，裴疆森. 3-羟基丙酸产生菌株的筛选及鉴定［J］. 食品与发酵工业，2010，36(4)：28-31.
［15］Valdehuesa K N，Liu H W，Nisola G M，et al. Recent advances in the metabolic engineering of microorganisms for the production of 3-hydroxypropionic acid as C3 platform chemical［J］. Applied Microbiology and Biotechnology，2013，97(8)：3309-3321.
［16］Bisaria V S，Kondo A. Microbial production of 3-hydroxypropionic acid from renewable sources：a green approach as an alternative to conventional chemistry［M］//Kumar V，Ashok S，Park S. Bioprocessing of renewable resources to commodity bioproducts. Hoboken，USA：Wiley，2014：381-407.

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备注/Memo

备注/Memo:
收稿日期：2014-08-26
基金项目：洛阳师范学院省部级以上项目培育基金(编号：10001423)；洛阳师范学院博士科研启动基金(编号：10001399)。
作者简介：吴孔阳(1985—)，男，河南商城人，博士，讲师，主要从事食品微生物研究。E-mail：kywu2007@126.com。
更新日期/Last Update: 2015-09-25