|本期目录/Table of Contents|

[1]郜卫华,田罗,黄廷华,等.凡纳滨对虾钙网蛋白cDNA序列的克隆及组织表达分析[J].江苏农业科学,2016,44(01):36-40,47.
　Gao Weihua,et al.Cloning，characterization and expression of full length cDNA in calreticulin of Litopenaeus vannamei[J].Jiangsu Agricultural Sciences,2016,44(01):36-40,47.
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凡纳滨对虾钙网蛋白cDNA序列
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年01期
页码:
36-40,47
栏目:
生物技术
出版日期:
2016-01-25

文章信息/Info

Title:
Cloning，characterization and expression of full length cDNA in calreticulin of Litopenaeus vannamei
作者:
郜卫华1; 2;  田罗3;  黄廷华1;  姚敏1;  许巧情1; 2
1.长江大学动物科学学院水产系，湖北荆州 434025；2.汕头大学广东省海洋生物技术重点实验，广东汕头 515063；
3.荆州职业技术学院，湖北荆州 434023
Author(s):
Gao Weihua; et al
关键词:
凡纳滨对虾; 钙网蛋白; 基因克隆; 表达
Keywords:
-
分类号:
S917.4
DOI:
-
文献标志码:
A
摘要:
利用RACE技术，克隆了凡纳滨对虾钙网蛋白(calreticulin，CRT)基因(GenBank登录号：JQ682618)。该基因(LvCRT)cDNA全长1 866 bp，含有1个长达1 221 bp的完整开放阅读框(ORF)，编码的成熟肽由406个氨基酸组成，5′UTR(5′非编码区)长度为222 bp，3′UTR长度为423 bp，3′端加尾信号AATAAA位于poly(A)尾巴上游27 bp处。预测其理论分子量是15.3 ku，等电点pI为4.90。具有1个保守的钙网蛋白家族标签(KHEQNIDCGGGYLKVF)，1个信号肽(MKTWVFLALFGVVLVES)和保守的HDEL内质网回收标签。经NCBI BLASTX比对表明，LvCRT基因与中国明对虾和斑节对虾的LvCRT具有高度的相似性和一致性。系统进化分析表明，LvCRT在亲缘关系上更接近昆虫的钙网蛋白基因。荧光定量PCR结果显示CRT在肌肉组织中表达量最低，在肠中的表达量最高。对凡纳滨对虾CRT基因全长cDNA序列克隆和表达的研究为更进一步了解CRT多肽在凡纳滨对虾中的重要功能奠定了基础。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2015-07-12
基金项目：广东省海洋生物技术重点实验室开放基金(编号：GPKLMB201403)；长江大学博士启动经费(编号：8012000101122)。
作者简介：郜卫华(1977—)，女，湖北襄阳人，博士，讲师，主要从事水产分子营养学研究。E-mail：gwh105@126.com。
通信作者：许巧情，博士，教授，主要从事水产分子生物学研究。E-mail：35507883@qq.com。
更新日期/Last Update: 2016-01-25