镉诱导锦鲤肾脏氧化性DNA损伤<br/>及谷胱甘肽抗氧化系统改变-《江苏农业科学》

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Title:: Cadmium induced renal oxidative DNA damage and glutathione antioxidant system change of brocade carp

作者:: 刘思思¹; 高旋旋²; 胡竹青²; 程超¹; 渠漫漫²; 孙晴²; 刘缠民¹; 郑桂红¹; 1.江苏师范大学，江苏徐州 221116；2.江苏师范大学科文学院，江苏徐州 221116

Author(s):: Liu Sisi; et al

关键词:: 锦鲤; 镉; 肾脏; DNA损伤; 氧化应激

Keywords:: -

分类号:: S941.91

DOI:: -

文献标志码:: A

摘要:: 研究镉(Cd)暴露对锦鲤(Carassius auratus L.)肾脏氧化应激、氧化性DNA损伤、谷胱甘肽抗氧化系统的影响。试验采用静水生物养殖法，将40尾锦鲤随机分为4组，每组10尾，分别暴露于0、5、10、20 mg/L的CdCl₂水溶液中，持续96 h。观察锦鲤的行为变化，测定肾脏丙二醛(MDA)含量、氧化性DNA指标8-羟基鸟嘌呤(8-OHdG)水平、还原型谷胱甘肽(GSH)水平，并检测谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽-S-转移酶(GST)、谷胱甘肽还原酶(GR)、谷氨酸半胱氨酸连接酶(GCL)的活性。结果表明，Cd暴露使锦鲤发生明显改变，MDA、8-OHdG、GSH水平均升高，并改变了谷胱甘肽抗氧化酶系统。

Abstract:: -

参考文献/References:

［1］陈再忠，沈芾，孔令富，等. 重金属镉在锦鲤各组织中的富集［J］. 江苏农业科学，2011，39(6)：387-389.
［2］Dabas A，Nagpure N S，Kumar R，et al. Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel，Channa punctatus［J］. Fish Physiology and Biochemistry，2012，38(2)：469-482.
［3］郑桂红，唐玲玲，孙建梅，等. 重金属锌对锦鲤组织氧化损伤的作用［J］. 江苏农业科学，2014，42(3)：187-189.
［4］Liu C M，Ma J Q，Sun Y Z. Protective role of puerarin on lead-induced alterations of the hepatic glutathione antioxidant system and hyperlipidemia in rats［J］. Food and Chemical Toxicology，2011，49(12)：3119-3127.
［5］曾艳艺，赖子尼，杨婉玲，等. 广东鲂幼鱼对铜、镉胁迫的氧化应激响应及其指示意义［J］. 生态环境学报，2014，23(12)：1972-1979.
［6］田鹏. 水体中镉暴露对草鱼的氧化胁迫研究［D］. 重庆：西南大学，2013.
［7］Sveceviius G. Behavioral responses of rainbow trout Oncorhynchus mykiss to sublethal toxicity of a model mixture of heavy metals［J］. Bulletin of Environmental Contamination and Toxicology，2005，74(5)：845-852.
［8］Liu C M，Zheng G H，Ming Q L，et al. Sesamin protects mouse liver against nickel-induced oxidative DNA damage and apoptosis by the PI3K-Akt pathway［J］. Journal of Agricultural and Food Chemistry，2013，61(5)：1146-1154.
［9］Stohs S J，Bagchi D，Hassoun E，et al. Oxidative mechanisms in the toxicity of chromium and cadmium ions［J］. Journal of Environmental Pathology Toxicology and Oncology，2000，19(3)：201-213.
［10］Schlenk D，Rice C D. Effect of zinc and cadmium treatment on hydrogen peroxide-induced mortality and expression of glutathione and metallothionein in a teleost hepatoma cell line［J］. Aquatic Toxicology，1998，43(2/3)：121-129.

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