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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第44卷

期数:

2016年04期

页码:

95-98

栏目:

生物技术

出版日期:

2016-04-25

文章信息/Info

Title:

Cloning and expression analysis of imaginal disc growth factor gene in Tenebrio molitor

作者:

赵满¹;  李丽芳¹;  王友竹¹;  Elena N. Elpidina²;  朱家颖¹

1.西南林业大学云南省森林灾害预警与控制重点实验室，云南昆明 650224；
2.A.N. Belozersky Institute of Physico-Chemical Biology，Moscow State University，Moscow 119991，Russia

Author(s):

Zhao Man; et al

关键词:

翅芽生长因子; 基因克隆; 黄粉甲; 序列分析; 管氏肿腿蜂

Keywords:

-

分类号:

S433.5

DOI:

-

文献标志码:

A

摘要:

翅芽生长因子(imaginal disc growth factor，IDGF)是一类调节昆虫发育和生长的因子。利用RACE克隆技术，克隆获得黄粉甲(Tenebrio molitor) IDGF基因，其cDNA序列长1 465 bp，开放阅读框为1 296 bp，可编码431个氨基酸，预测理论分子量为48.25 ku，等电点为7.63。氨基酸序列同源比对分析发现，黄粉甲IDGF与赤拟谷盗(Tribolium castaneum)和蔗根非耳象(Diaprepes abbreviatus)的IDGF相似性分别为89%、71%。系统发育树分析表明，黄粉甲IDGF与赤拟谷盗IDGF进化关系最近。荧光定量PCR分析表明，在不同发育阶段，IDGF基因在幼虫1龄和2龄中的表达量明显高于其他发育阶段。在蛹期不同组织中，IDGF基因在脂肪体中的表达量最高。被管氏肿腿蜂(Scleroderma guani)寄生后，IDGF基因的转录水平未受影响，表明寄生不能调控该基因的转录表达。

Abstract:

-

参考文献/References:

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备注/Memo

备注/Memo:

收稿日期：2015-09-23
基金项目：国家自然科学基金(编号：31411130192、31260449)；云南省中青年学术与技术带头人后备人才项目(编号：2013HB077)；云南省高校森林有害生物科技创新团队项目。
作者简介：赵满(1988—)，女，云南昭通人，硕士研究生，主要从事昆虫生理生化与分子生物学研究。E-mail：691041481@qq.com。
通信作者：朱家颖，博士，副教授，主要从事昆虫生理生化与分子生物学研究。E-mail：jyzhu001@gmail.com。

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更新日期/Last Update: 2016-04-25