|本期目录/Table of Contents|

[1]徐小勇,许晓玲,刘玉玲,等.椪柑原生质体培养过程中羟自由基和总活性氧水平的分析[J].江苏农业科学,2016,44(05):196-198.
　Xu Xiaoyong,et al.Analysis of hydroxyl radical and total active oxygen levels in ponkan (Citrus reticulata Blanco) protoplasts during culture[J].Jiangsu Agricultural Sciences,2016,44(05):196-198.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年05期
页码:
196-198
栏目:
园艺与林学
出版日期:
2016-05-25

文章信息/Info

Title:
Analysis of hydroxyl radical and total active oxygen levels in ponkan (Citrus reticulata Blanco) protoplasts during culture
作者:
徐小勇;  许晓玲;  刘玉玲;  何丽;  张一卉;  陈翔
扬州大学，江苏扬州 225009
Author(s):
Xu Xiaoyong; et al
关键词:
椪柑; 原生质体; 羟自由基; 活性氧
Keywords:
-
分类号:
S666.104+.3
DOI:
-
文献标志码:
A
摘要:
以椪柑愈伤组织和试管苗为材料，通过酶解分离原生质体，于液体培养和观察；采用羟自由基测定试剂盒和电子顺磁共振技术分析羟自由基变化，通过流式细胞仪检测总活性氧水平变化。结果发现，培养的前3 d，愈伤组织原生质体羟自由基和总活性氧水平下降，而叶肉原生质体则升高；培养的后3 d，除愈伤组织原生质体羟自由基水平升高外，其他都下降。椪柑叶肉原生质体可能由于氧化胁迫引起死亡，而愈伤组织原生质体能较好地控制总活性氧水平从而顺利进入再生，另外培养6 d时其羟自由基含量的升高可能与细胞壁再生等有关。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2015-03-31
基金项目：国家自然科学基金(编号：31372025)；江苏省自然科学基金(编号：BK20131228)；扬州大学研究生科研创新计划(编号：CXLX_1425)。
作者简介：徐小勇(1979—)，男，江西奉新人，博士，副教授，主要从事果树生物技术研究。E-mail：xyxu@yzu.edu.cn。
更新日期/Last Update: 2016-05-25