|本期目录/Table of Contents|

[1]艾子凌,高鹏,杜黎黎,等.利用CAPS初步定位甜瓜MR-1白粉病抗性基因[J].江苏农业科学,2016,44(06):66-70.
 Ai Ziling,et al.Location of powdery mildew resistant gene using CAPS makers in melon MR-1[J].Jiangsu Agricultural Sciences,2016,44(06):66-70.
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利用CAPS初步定位甜瓜MR-1白粉病抗性基因(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年06期
页码:
66-70
栏目:
生物技术
出版日期:
2016-06-25

文章信息/Info

Title:
Location of powdery mildew resistant gene using CAPS makers in melon MR-1
作者:
艾子凌 高鹏 杜黎黎 栾非时
东北农业大学园艺学院/农业部东北地区园艺作物生物学与种质创制重点实验室,黑龙江哈尔滨 150030
Author(s):
Ai Zilinget al
关键词:
甜瓜白粉病生理小种CAPS遗传图谱
Keywords:
-
分类号:
S436.5
DOI:
-
文献标志码:
A
摘要:
从国际生理小种鉴别寄主中选取甜瓜抗病自交系MR-1为母本,感病自交系Topmark为父本,构建F2代群体。对354株F2代群体进行田间甜瓜白粉病抗病性调查并分析其遗传规律,发现甜瓜白粉病的抗性基因为单显性基因;根据两亲本基因组重测序数据,自行开发设计CAPS分子标记,将具有多态性的标记应用于F2代基因分型以构建遗传连锁图谱。图谱内含142个CAPS标记,分布于12个连锁群上。图谱总长度2 065.47 cM,平均距离14.55 cM;标记最多的为第四连锁群,分布22个标记,标记间平均距离为11.61 cM;最长的为第五连锁群,总长277.98 cM;最短的为第三连锁群,总长65.6 cM。得到1个甜瓜白粉病抗性相关基因位点,该位点在第七连锁群上,位于CAPS标记7-4E和7-1H之间。
Abstract:
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参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-03-11
基金项目:国家自然科学基金(编号:31301791);农业部“948”项目(编号:2014-S15)。
作者简介:艾子凌(1988—),女,黑龙江齐齐哈尔人,硕士研究生,主要从事甜瓜分子育种。 E-mail:linglingfighting@126.com。
通信作者:栾非时,教授,博士生导师,主要从事西甜瓜分子遗传育种。 E-mail:luanfeishi@sina.com。
更新日期/Last Update: 2016-06-25