|本期目录/Table of Contents|

[1]刘蒲临,程德勇,缪礼鸿.产几丁质酶侧孢短芽孢杆菌M64的产酶条件优化及部分酶学性质研究[J].江苏农业科学,2016,44(10):468-471.
　Liu Pulin,et al.Study on enzyme production condition optimization and some enzymatic properties of chitinase-producing Brevibacillus laterosporu M64[J].Jiangsu Agricultural Sciences,2016,44(10):468-471.
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产几丁质酶侧孢短芽孢杆菌M64的产酶条件优化及部分酶学性质研究(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年10期
页码:
468-471
栏目:
资源与环境
出版日期:
2016-10-25

文章信息/Info

Title:
Study on enzyme production condition optimization and some enzymatic properties of chitinase-producing Brevibacillus laterosporu M64
作者:
刘蒲临;  程德勇;  缪礼鸿
武汉轻工大学生物与制药工程学院，湖北武汉 430023
Author(s):
Liu Pulin; et al
关键词:
侧孢短芽孢杆菌; 几丁质酶; 产酶条件; 酶学性质
Keywords:
-
分类号:
S182
DOI:
-
文献标志码:
A
摘要:
在紫外诱变的基础上，采用单因素试验和正交试验对侧孢短芽孢杆菌(Brevibacillus laterosporus) M64发酵生产几丁质酶的培养条件和培养基进行优化，并对粗酶液的酶学特性进行了初步研究。结果表明，碳氮源的种类与浓度、培养温度、pH值以及表面活性剂吐温80浓度对该菌株产几丁质酶有显著影响。突变株使用优化后的培养条件，其发酵上清液中几丁质酶活力达到55.19 U/mL，为未优化前的2.56倍；粗酶液最适催化温度为60 ℃，且在80 ℃预处理2 h后仍具有降解几丁质的能力，表现出了良好的热稳定性。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2015-09-29
基金项目：国家“863”高技术研究发展计划(编号：2013AA102805)；武汉轻工大学科研启动经费(编号：2014RZ19)。
作者简介：刘蒲临(1985—)，男，湖北武汉人，博士，讲师，主要从事环境微生物学研究。Tel：(027)83956793；E-mail：liunan3585@163.com。
通信作者：缪礼鸿，博士，教授，主要从事资源与环境微生物学研究。Tel：(027)83956793；E-mail：miaowhpu@126.com。
更新日期/Last Update: 2016-10-25