|本期目录/Table of Contents|

[1]郑春花,孔祥远,隋炯明,等.花生金属蛋白酶家族基因FtsH的鉴定、分类和盐胁迫表达分析[J].江苏农业科学,2016,44(12):74-77.
　Zheng Chunhua,et al.Identification，clustering and response to salinity stress of metalloprotease FtsH genes in peanut[J].Jiangsu Agricultural Sciences,2016,44(12):74-77.
点击复制

花生金属蛋白酶家族基因FtsH的鉴定、
分类和盐胁迫表达分析(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年12期
页码:
74-77
栏目:
生物技术
出版日期:
2016-12-25

文章信息/Info

Title:
Identification，clustering and response to salinity stress of metalloprotease FtsH genes in peanut
作者:
郑春花; 孔祥远; 隋炯明; 束晨; 赵春梅
青岛农业大学/山东省高校植物生物技术重点实验室，山东青岛 266109
Author(s):
Zheng Chunhua; et al
关键词:
花生; FtsH基因; 基因表达谱; 耐盐突变体; 盐胁迫
Keywords:
-
分类号:
S565.201
DOI:
-
文献标志码:
A
摘要:
FtsH是一种ATP和Zn1，2，32+依赖型金属蛋白酶，在植物抗逆胁迫中发挥了重要作用。为分析花生中FtsH家族成员情况，构建花生叶片转录组数据文库，筛选出19个FtsH家族基因，位于花生A组野生种的8条染色体上，与拟南芥、水稻的FtsH基因进行同源序列比对后发现，多数FtsH基因没有聚到已报道的亚类。利用花生耐盐突变体(S2)和对照(S4)构建盐胁迫处理前后各时间段的表达谱文库，进行FtsH基因盐胁迫表达分析，结果表明，9个FtsH基因受盐胁迫诱导表达，绝大多数FtsH基因在耐盐突变体和对照中表现出不同的表达模式。该研究为花生金属蛋白酶基因的功能研究与耐盐分子育种提供了基础。
Abstract:
-

参考文献/References:

［1］Schumann W. FtsH-a single-chain charonin［J］. FEMS Microbiol Rev，1999，23(1)：1-11.
［2］Ostersetzer O，Adam Z. Light-stimulated degradation of an unassembled Rieske FeS protein by a thylakoid-bound protease：the possible role of the FtsH protease［J］. Plant Cell，1997，9(6)：957-965.
［3］Akiyama Y，Kihara A，Ito K. Subunit ɑ of proton ATPase F0 sector is a substrate of the FtsH protease in Escherichia coli［J］. FEBS Lett，1996，399：26-28.
［4］Lindahl M，Spetea C，Hundal T，et al. The thylakoid FtsH protease plays a role in the light-induced turnover of the photosystem Ⅱ D1 protein［J］. Plant Cell，2000(12)：419-431.
［5］Akiyama Y，Shirai Y，Ito K. Involvement of FtsH in protein assembly into and through the membrane. Ⅱ. Dominant mutations affecting FtsH functions［J］. J Biol Chem，1994，269(7)：5225-5229.
［6］Jayasekera M M，Foltin S K，Olson E R，et al. Escherichia coli requires the protease activity of FtsH for growth［J］. Arch Biochem Biophys，2000，380：103-107.
［7］Makino S，Makinoa T，Abe K，et al. Second transmembrane segment of FtsH plays a role in its proteolytic activity and homo-oligomerization［J］. FEBS Letters，1999，460(3)：554-558.
［8］张杰道，孙爱清. 拟南芥和水稻金属蛋白酶FtsH基因家族的基因组比较分析［J］. 生物工程学报，2009，25(9)：1402-1408.
［9］Ivashuta S，Imai R，Uchiyama K，et al. Changes in chloroplast FtsH-like gene during cold acclimation in alfalfa (Medicago sativa)［J］. J Plant Physiol，2002，159：85-90.
［10］Sun A Q，Yi S Y，Yang J Y，et al. Identification and characterization of a heat-inducible FtsH gene from tomato (Lycopersicon esculentum Mill.)［J］. Plant Sci，2006，170：551-562.
［11］Kore-eda S，Cushman M A，Akselrod I，et al. Transcript profiling of salinity stress responses by large-scale expressed sequence tag analysis in Mesembryanthemum crystallinum［J］. Gene，2004，341：83-92.
［12］Sui J M，Wang Y，Wang P，et al. Generation of peanut drought tolerant plants by pingyangmycin-mediated in vitro mutagenesis and hydroxyproline-resistance screening［J］. PLoS One，2015，10(3)：e0119240.
［13］Seo S，Okamoto M，Iwai T，et al. Reduced levels of chloroplast FtsH protein in tobacco mosaic virus-infected tobacco leaves accelerate the hypersensitive reaction［J］. Plant Cell，2000，12：917-932.
［14］Chen M，Choi Y，Voytas D F，et al. Mutations in the Arabidopsis VAR2 locus cause leaf variegation due to the loss of a chloroplast FtsH protease［J］. Plant J，2000，22：303-313.
［15］Sakamoto W，Tamura T，Hanba-Tomita Y，et al. The VAR1 locus of Arabidopsis encodes a chloroplastic FtsH and is responsible for leaf variegation in the mutant alleles［J］. Genes Cells，2002(7)：769-780.

相似文献/References:

[1]王有宁,赵丽艳,章爱群,等.花生高光谱叶片营养诊断研究[J].江苏农业科学,2014,42(12):129.
　Wang Youning,et al.Study on nutrition diagnosis of peanut leaves based on hyperspectral data[J].Jiangsu Agricultural Sciences,2014,42(12):129.
[2]刘永惠,沈一,陈志德.花生种质苗期抗旱性鉴定与评价[J].江苏农业科学,2014,42(11):108.
　Liu Yonghui,et al(08).Identification and evaluation of drought resistance of peanut germplasm during seeding stage[J].Jiangsu Agricultural Sciences,2014,42(12):108.
[3]张俊,刘娟,汤丰收,等.不同花生品种抗旱生理特性的差异[J].江苏农业科学,2015,43(12):114.
　Zhang Jun,et al.Difference of drought-resistant physiological characteristics of different peanut varieties[J].Jiangsu Agricultural Sciences,2015,43(12):114.
[4]刘一佳,任学敏,朱雅,等.施氮水平对花生冠层温度和产量性状的影响及其相互关系[J].江苏农业科学,2015,43(12):101.
　Liu Yijia,et al.Effects of nitrogen rate on canopy temperature and yield characteristics of peanut and their relationship[J].Jiangsu Agricultural Sciences,2015,43(12):101.
[5]杨洁,张勇,杨萍,等.山东省花生产业发展现状分析[J].江苏农业科学,2014,42(09):433.
　Yang Jie,et al.Analysis of industrial development of peanuts in Shandong Province[J].Jiangsu Agricultural Sciences,2014,42(12):433.
[6]张永平,乔永旭,赵绪明,等.蚯蚓粪作基肥对夏播花生生长与产量的影响[J].江苏农业科学,2014,42(08):97.
　Zhang Yongping,et al.Effects of vermicompost as basal fertilizer on growth and yield of summer-sowing peanut[J].Jiangsu Agricultural Sciences,2014,42(12):97.
[7]徐静,张新友,汤丰收,等.花生种间杂交胚胎发育初探[J].江苏农业科学,2013,41(09):73.
　Xu Jing,et al.Preliminary study on embryo development in interspecific hybrid of peanut[J].Jiangsu Agricultural Sciences,2013,41(12):73.
[8]王晓军,孙东雷,王宗标,等.江苏省花生地方品种农艺性状分析[J].江苏农业科学,2013,41(09):77.
　Wang Xiaojun,et al.nomic traits analysis of peanut landraces in Jiangsu Province[J].Jiangsu Agricultural Sciences,2013,41(12):77.
[9]崔少雄,王雪,孙志梅,等.不同产量水平花生品种的生长发育特性比较[J].江苏农业科学,2016,44(03):107.
　Cui ShaoXiong,et al.Comparison of growth and development characteristics of different peanut varieties based on yield levels[J].Jiangsu Agricultural Sciences,2016,44(12):107.
[10]景令怡,朱珏,乔辉.农村“空壳化”对花生种植户生产效率的影响机制研究[J].江苏农业科学,2016,44(03):480.
　Jing Lingyi,et al.Study on effect of phenomenon of “empty village” on productivity and efficiency of peanut growers[J].Jiangsu Agricultural Sciences,2016,44(12):480.

备注/Memo

备注/Memo:
收稿日期：2016-08-30
基金项目：国家自然科学基金(编号：31571705、31301356、31471542)；山东省科技发展计划(编号：2014GNC110002)。
作者简介：郑春花(1979—)，女，山西忻州人，硕士，图书馆员，主要从事生物信息学分析。E-mail：zchsjm@163.com。
通信作者：赵春梅，博士，副教授，主要从事作物分子育种研究。E-mail：meiwei2002@163.com。
更新日期/Last Update: 2016-12-25