|本期目录/Table of Contents|

[1]赵为民,涂枫,任守文,等.激活素A对大鼠肌细胞增殖分化的作用[J].江苏农业科学,2016,44(12):287-289.
　Zhao Weimin,et al.Roles of activin A on proliferation and differentiation of rat myoblasts[J].Jiangsu Agricultural Sciences,2016,44(12):287-289.
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激活素A对大鼠肌细胞增殖分化的作用(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年12期
页码:
287-289
栏目:
畜牧兽医与水产蚕桑
出版日期:
2016-12-25

文章信息/Info

Title:
Roles of activin A on proliferation and differentiation of rat myoblasts
作者:
赵为民; 涂枫; 任守文; 方晓敏; 李碧侠; 赵芳; 付言峰; 王学敏
江苏省农业科学院畜牧研究所/江苏省农业科学院动物品种改良和繁育重点实验室，江苏南京 210014
Author(s):
Zhao Weimin; et al
关键词:
大鼠; 激活素AActivin A; 肌细胞; 增殖; 细胞周期
Keywords:
-
分类号:
S828.2
DOI:
-
文献标志码:
A
摘要:
激活素AActivin A是转化生长因子β(transforming/mL growth factor-β，TGF-β)超家族中的一员，具有重要的生物学功能。为研究Activin A在肌肉发育中的作用，以大鼠肌细胞L6为研究模型，通过时空表达谱、CCK-8以及流式细胞周期分析等方法来进行研究。结果表明Activin A随着肌细胞由增殖转向分化状态，其表达量呈下降趋势。添加不同浓度的Activin A5~25 ng/mL均能显著促进L6细胞的增殖速度，且这种促进细胞增殖的效应随着Activin A浓度的增大而逐渐增强。Activin A能显著增加S期在细胞周期中的比例，同时缩短G1和G2期在细胞周期中所占的比例。此外，通过添加Activin A显著抑制了肌肉分化标记基因Myog的表达。以上结果为进一步研究 Activin A在肌肉发育中的作用机制奠定了基础。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2016-09-12
基金项目：国家转基因生物新品种培育重大专项编号：2014ZX08006-003；国家生猪现代产业技术体系建设专项编号：CARS-36。
作者简介：赵为民1983—，男，湖北钟祥人，博士，助理研究员，主要从事转基因与育种研究。Tel：02584391941；E-mail：zhao_weimin1983@aliyun.com。
通信作者：王学敏，硕士，助理研究员，主要从事转基因与猪生产研究。Tel：02584391941；E-mail：wxm116@sina.com。
更新日期/Last Update: 2016-12-25