活性氧自由基在试验性乳腺炎大鼠发病机制中的作用-《江苏农业科学》

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Title:: Effect of reactive oxygen species on pathogenesis of experimental mastitis in rats

作者:: 顾蓓蓓¹; 2; 卢劲晔¹; 马卉²; 卢炜¹; 刘静¹; 1.江苏农牧科技职业学院，江苏泰州 225300；2.泰州出入境检验检疫局，江苏泰州 225300

Author(s):: Gu Beibei; et al

关键词:: 活性氧; 中性粒细胞; 乳腺炎; 大鼠

Keywords:: -

分类号:: R979.5

DOI:: -

文献标志码:: A

摘要:: 为探讨活性氧自由基在宿主抵抗乳腺感染中的作用，选取36只清洁级SD怀孕大鼠，于产后72 h经乳头管灌注10 μg/侧脂多糖(lipopolysaccharides，LPS)至第4对乳腺(两侧)内。分别于灌注前(定义为0 h)及灌注后2、4、8、16、24 h(n=6)颈静脉放血处死动物，采集样品。结果显示，LPS诱发乳腺炎引起白细胞介素-8(interleukin-8，IL-8)及细胞间黏附分子-1(intercellular adhesion molecule-1，ICAM-1)表达显著升高，从而促进嗜中性粒细胞(neutrophil，PMN)迁徙及活性氧自由基(reactive oxygen species，ROS)的释放。乳腺组织N-乙酰-β-D-氨基葡萄糖苷酶(N-acetyl-β-D-glucosaminidase，NAGase)活力与ROS释放变化结果一致，LPS灌注后各时间点均显著升高，并于灌注后8 h达到峰值。结果表明，PMN是宿主抵御乳腺感染的主要效应细胞，通过释放ROS消灭病原菌，但大量释放的ROS也是引起组织损伤的重要原因。

Abstract:: -

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