|本期目录/Table of Contents|

[1]尚秋萍,黄林,胡雨薇,等.牦牛和犏牛睾丸中泛素结合酶基因Ube2n、泛素连接酶基因Trim36表达水平的比较[J].江苏农业科学,2017,45(01):19-21.
 Shang Qiuping,et al.Comparison of expression levels of ubiquitin enzymes Ube2n and Trim36 in testes of yak and sterile cattle-yak[J].Jiangsu Agricultural Sciences,2017,45(01):19-21.
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牦牛和犏牛睾丸中泛素结合酶基因Ube2n
泛素连接酶基因Trim36表达水平的比较
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年01期
页码:
19-21
栏目:
生物技术
出版日期:
2017-01-05

文章信息/Info

Title:
Comparison of expression levels of ubiquitin enzymes Ube2n and Trim36 in testes of yak and sterile cattle-yak
作者:
尚秋萍 黄林 胡雨薇 雷杰雯 金素钰 郑玉才
西南民族大学生命科学与技术学院,四川成都 610041
Author(s):
Shang Qiupinget al
关键词:
牦牛杂交雄性不育睾丸蛋白质降解泛素化
Keywords:
-
分类号:
S823.82;Q786
DOI:
-
文献标志码:
A
摘要:
为探讨蛋白质泛素化与牦牛杂交后代睾丸精子发生异常的相关性,比较了参与泛素化的2种酶基因(泛素结合酶基因Ube2n、泛素连接酶基因Trim36)在成年牦牛和雄性不育犏牛睾丸中的mRNA水平。定量PCR分析显示,牦牛(n=9)睾丸中Ube2n、Trim36基因的mRNA水平分别显著、极显著高于犏牛(n=7),其中Trim36 mRNA水平相差10倍以上。研究结果提示,犏牛睾丸蛋白质泛素化水平明显下降,可能会影响精子发生过程中正常的蛋白质更新。
Abstract:
-

参考文献/References:

[1]张旭静. 牦牛和普通牛种间杂种公牛睾丸的组织学观测与研究[J]. 畜牧兽医学报,2001,32(4):314-318.
[2]屈旭光,李齐发,刘振山,等. 牦牛、犏牛睾丸组织中SYCP3基因mRNA表达水平研究[J]. 畜牧兽医学报,2008,39(8):1132-1136.
[3]付永,魏雅萍,吴克选,等. Real-time PCR检测黄牛、牦牛、犏牛睾丸组织中Boule、Dazl基因mRNA表达[J]. 生物技术通报,2012(10):150-155.
[4]金帅,郭宪,包鹏甲,等. 牦牛和犏牛Dmrt7基因序列分析及其在睾丸组织中的表达水平[J]. 中国农业科学,2013,46(5):1036-1043.
[5]李贤,李齐发,赵兴波,等. 牦牛和犏牛Dmc1基因序列分析及睾丸组织转录水平研究[J]. 中国农业科学,2010,43(15):3221-3229.
[6]付伟,李彩霞,刘文静,等. 利用双向电泳-质谱技术鉴定牦牛和犏牛睾丸中的差异表达蛋白质[J]. 西南民族大学学报(自然科学版),2014,40(1):11-15.
[7]Lou Y N,Liu W J,Wang C L,et al. Histological evaluation and Prdm9 expression level in the testis of sterile male cattle-yaks[J]. Livestock Science,2014,160:208-213.
[8]Komander D,Rape M. The ubiquitin code[J]. Annual Review of Biochemistry,2012,81:203-229.
[9]何玲,张令强. 线性泛素化修饰研究进展[J]. 遗传,2015,37(9):911-917.
[10]Li X C,Barringer B C,Barbash D A. The pachytene checkpoint and its relationship to evolutionary patterns of polyploidization and hybrid sterility[J]. Heredity,2009,102(1):24-30.
[11]Shang Z F,Tan W,Liu X D,et al. DNA-PKcs negatively regulates cyclin B1 protein stability through facilitating its ubiquitination mediated by Cdh1-APC/C pathway[J]. International Journal of Biological Sciences,2015,11(9):1026-1035.
[12]Jiang Z H,Dong X W,Shen Y C,et al. DNA damage regulates ARID1A stability via SCF ubiquitin ligase in gastric cancer cells[J]. European Review for Medical and Pharmacological Sciences,2015,19(17):3194-3200.

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备注/Memo

备注/Memo:
收稿日期:2015-09-26
基金项目:国家自然科学基金(编号:31240053)。
作者简介:尚秋萍(1992—),女,湖南吉首人。E-mail:m18782278962@163.com。
通信作者:郑玉才,博士,教授,主要从事泌乳生物化学、高原家畜生化与分子遗传学研究。E-mail:yucaizheng65@hotmail.com。
更新日期/Last Update: 2017-01-05