|本期目录/Table of Contents|

[1]李夏莹,张秀杰,宋贵文.STAT3蛋白真核表达载体的构建及其泛素化功能研究[J].江苏农业科学,2017,45(11):16-18.
　Li Xiaying,et al.Study on construction of eukaryotic expression vector of STAT3 protein and its ubiquitination function[J].Jiangsu Agricultural Sciences,2017,45(11):16-18.
点击复制

STAT3蛋白真核表达载体的构建及其泛素化功能研究(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年11期
页码:
16-18
栏目:
生物技术
出版日期:
2017-06-05

文章信息/Info

Title:
Study on construction of eukaryotic expression vector of STAT3 protein and its ubiquitination function
作者:
李夏莹;  张秀杰;  宋贵文
农业部科技发展中心，北京 100176
Author(s):
Li Xiaying; et al
关键词:
STAT3蛋白; 表达载体; 泛素化; K63; K48
Keywords:
-
分类号:
Q78
DOI:
-
文献标志码:
A
摘要:
为研究信号转导与转录激活因子3(STAT3)的活性调控机制，克隆人源STAT3基因，并将其构建在真核表达载体上。之后，通过细胞内共表达STAT3和泛素化质粒的方法，验证了STAT3蛋白可以发生多种类型的泛素化修饰。结果表明，K63位非降解功能的泛素化影响STAT3蛋白的磷酸化，从而影响STAT3的活性及功能的发挥；而K48位降解功能的泛素化影响STAT3蛋白的降解，使STAT3蛋白得以更新，这2种类型的泛素化对STAT3蛋白及其功能都十分重要。
Abstract:
-

参考文献/References:

［1］Levy D E，Kessler D S，Pine R，et al. Interferon-induced nuclear factors that bind a shared promoter element correlate with positive and negative transcriptional control［J］. Genes & Development，1988，2(4)：383-393.
［2］Dale T C，Imam A M，Kerr I M，et al. Rapid activation by interferon alpha of a latent DNA-binding protein present in the cytoplasm of untreated cells［J］. Proceedings of the National Academy of Sciences of the United States of America，1989，86(4)：1203-1207.
［3］Fu X Y. A transcription factor with SH2 and SH3 domains is directly activated by an interferon alpha-induced cytoplasmic protein tyrosine kinase(s)［J］. Cell，1992，70(2)：323-335.
［4］Nakanoh S，Fuse N，Tadokoro R，et al. Jak1/Stat3 signaling acts as a positive regulator of pluripotency in chicken pre-gastrula embryos［J］. Developmental Biology，2017，421(1)：43-51.
［5］Yu H，Pardoll D，Jove R. STATs in cancer inflammation and immunity：a leading role for STAT3［J］. Nature Reviews Cancer，2009，9(11)：798-809.
［6］Larner A C，David M，Feldman G M，et al. Tyrosine phosphorylation of DNA binding proteins by multiple cytokines［J］. Science，1993，261(5129)：1730-1733.
［7］Lee H，Zhang P，Herrmann A，et al. Acetylated STAT3 is crucial for methylation of tumor-suppressor gene promoters and inhibition by resveratrol results in demethylation［J］. Proceedings of the National Academy of Sciences of the United States of America，2012，109(20)：7765-7769.
［8］Pan J，Lee Y，Zhang Q，et al. Honokiol decreases lung cancer metastasis through inhibition of the STAT3 signaling pathway［J］. Cancer Prevention Research，2017，10(2)：133-141.
［9］Park S，Kim D，Kaneko S，et al. Molecular cloning and characterization of the human AKT1 promoter uncovers its up-regulation by the Src/Stat3 pathway［J］. Journal of Biological Chemistry，2005，280(47)：38932-38941.
［10］Xu P，Duong D M，Seyfried N T，et al. Quantitative proteomics reveals the function of unconventional ubiquitin chains in proteasomal degradation［J］. Cell，2009，137(1)：133-145.
［11］Rieser E，Cordier S M，Walczak H. Linear ubiquitination：a newly discovered regulator of cell signalling［J］. Trends in Biochemical Sciences，2013，38(2)：94-102.
［12］Yang W L，Jin G，Li C F，et al. Cycles of ubiquitination and deubiquitination critically regulate growth factor-mediated activation of Akt signaling［J］. Science Signaling，2013，6(257)：ra3.
［13］Lin K. The Akt DUBbed InAktive［J］. Science Signaling，2013，6(257)：Pel.
［14］Yang W L. The role of K63-linked ubiquitination cycles in Akt kinase activation［D］. Houston：The University of Texas at Houston，2013.
［15］Li P，Liu H，Zhang Y，et al. Endotoxin tolerance inhibits degradation of tumor necrosis factor receptor-associated factor 3 by suppressing pellino 1 expression and the K48 ubiquitin ligase activity of cellular inhibitor of apoptosis protein 2［J］. The Journal of Infectious Diseases，2016，214(6)：906-915.

相似文献/References:

[1]葛晓霞.柑橘CitSERK-LIKE基因原核表达载体的构建与表达[J].江苏农业科学,2016,44(04):22.
　Ge Xiaoxia.Construction of prokaryotic expression vector and expression of CitSERK1-like gene[J].Jiangsu Agricultural Sciences,2016,44(11):22.
[2]李慧,李爱,彭立新,等.杨树EBP1基因的克隆与表达载体构建[J].江苏农业科学,2015,43(04):14.
　Li Hui,et al.Cloning of EBP1 gene from populus and construction of its plant expression vector[J].Jiangsu Agricultural Sciences,2015,43(11):14.
[3]张学明,丁海麦,席海燕,等.重组人gdnf在人乳腺肿瘤上皮细胞中表达的研究[J].江苏农业科学,2015,43(02):16.
　Zhang Xueming,et al.Expression of human recombinant gdnf cDNA in human mammary tumor epithelial cell line Bcap-37[J].Jiangsu Agricultural Sciences,2015,43(11):16.
[4]裴华丽,刘永光,乔 宁,等.番茄黄化曲叶病毒Rep基因植物表达载体的构建及对番茄的遗传转化[J].江苏农业科学,2015,43(02):41.
　Pei Huali,et al.Construction of plant expression vector and genetic transformation of tomato yellow leaf curl virus Rep gene[J].Jiangsu Agricultural Sciences,2015,43(11):41.
[5]郑鹏,郑晓萌,于磊,等.牛DAZL基因序列分析、克隆及表达载体构建[J].江苏农业科学,2015,43(01):34.
　Zheng Peng,et al.Cloning，sequence analysis and expression vector construction of bovine DAZL gene[J].Jiangsu Agricultural Sciences,2015,43(11):34.
[6]段明.谷子EPSPS基因的分离、修饰及表达载体的构建[J].江苏农业科学,2016,44(05):51.
　Duan Ming.Separation， modification and construction of expression vector for millet EPSPS gene[J].Jiangsu Agricultural Sciences,2016,44(11):51.
[7]王红艳,王鸿磊.海滨锦葵耐盐基因KvSOS1的克隆与表达载体构建[J].江苏农业科学,2016,44(10):76.
　Wang Hongyan,et al.Cloning and expression vector construction of KvSOS1 from Kosteletzkya virginica L.[J].Jiangsu Agricultural Sciences,2016,44(11):76.

备注/Memo

备注/Memo:
收稿日期：2016-11-18
基金项目：国家科技重大专项(编号：2016ZX08012003)。
作者简介：李夏莹(1986—)，女，云南玉溪人，博士，农艺师，主要从事转基因检测技术方面的研究。E-mail：esmacloed006@163.com。
通信作者：宋贵文，高级农艺师，主要从事转基因生物安全方面的研究。Tel：(010)59198153；E-mail：songguiwen@agri.gov.cn。
更新日期/Last Update: 2017-06-05