|本期目录/Table of Contents|

[1]焦翠翠,郭妍妍,吴均章,等.水稻Ds标记的曲穗突变体的分子鉴定[J].江苏农业科学,2017,45(11):26-29.
 Jiao Cuicui,et al.Molecular identification of curved-panicle and Ds-tagged rice mutant[J].Jiangsu Agricultural Sciences,2017,45(11):26-29.
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水稻Ds标记的曲穗突变体的分子鉴定(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年11期
页码:
26-29
栏目:
生物技术
出版日期:
2017-06-05

文章信息/Info

Title:
Molecular identification of curved-panicle and Ds-tagged rice mutant
作者:
焦翠翠 郭妍妍 吴均章 孙丙耀
苏州大学医学部基础医学与生物科学学院,江苏苏州 215123
Author(s):
Jiao Cuicuiet al
关键词:
水稻穗型遗传发育改良Ulp1Ac/Ds3′-RACE技术
Keywords:
-
分类号:
S511.01
DOI:
-
文献标志码:
A
摘要:
在经过Ac/Ds基因标签系统获得的水稻Ds插入突变株系中得到1株水稻曲穗突变体植株,对该突变体进行初步表型观察,结果显示,成熟期突变体植株的稻穗有一定的弯曲;同时采用TAIL-PCR方法获得曲穗突变体Ds侧翼基因序列,结果证明含有1个Ds插入位点;对Ds侧翼序列进行分析发现,Ds插入在2个基因之间,其下游是编码类泛素特异性蛋白酶1(Ulp1)的基因,该基因与穗的形成有一定的关联;利用RACE技术扩增Ds插入位点下游基因的cDNA的3′端序列,序列比对发现,Ds插入前后其下游基因序列无变化。预测Ds的插入影响了Ulp1基因的启动子区域,进而影响水稻的花序生长发育形成穗弯曲水稻突变体。本研究结果可能在穗型的遗传及发育以及改良优质高产水稻方面有一定的作用。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2016-03-14
基金项目:江苏省苏州市应用基础研究计划(农业部分)(编号:SYN201204)。
作者简介:焦翠翠(1990—),女,硕士,研究方向为植物结构与发育生物学。E-mail:jcc651362527@163.com。
通信作者:孙丙耀,副教授,研究方向为植物分子遗传学。Tel:(0512)65882833;E-mail:sunbingyao@suda.edu.cn。
更新日期/Last Update: 2017-06-05