|本期目录/Table of Contents|

[1]陶宏卫,郭广富,曹军平,等.猪圆环病毒Ⅱ型盐城株的全基因组克隆与序列分析[J].江苏农业科学,2018,46(16):39-41.
 Tao Hongwei,et al.Cloning and sequence analysis of Yancheng isolates of porcine circovirus type 2[J].Jiangsu Agricultural Sciences,2018,46(16):39-41.
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猪圆环病毒Ⅱ型盐城株的全基因组克隆与序列分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年第16期
页码:
39-41
栏目:
生物技术
出版日期:
2018-08-30

文章信息/Info

Title:
Cloning and sequence analysis of Yancheng isolates of porcine circovirus type 2
作者:
陶宏卫1 郭广富1 曹军平1 申建荣2 张晋川1 练国俊1 朱爱萍1 张佳浩1 朱金其3
1.江苏农牧科技职业学院动物医学院,江苏泰州 225300; 2.江苏泰州泰牧动物医院,江苏泰州 225300;
3.江苏滨海鸿盛生猪饲养有限公司,江苏滨海 224500
Author(s):
Tao Hongweiet al
关键词:
猪圆环病毒Ⅱ型全基因组序列分析
Keywords:
-
分类号:
S852.65;S858.285.3
DOI:
-
文献标志码:
A
摘要:
采用PCR方法对采集自盐城地区的疑似猪圆环病毒病病料进行猪圆环病毒Ⅱ型(PCV 2)全基因组的扩增,确定了2份病料中的PCV 2的分子特征,分别命名为BH07株和BH11株,对其进行克隆、测序后,应用软件进行全基因组核苷酸序列比对分析。结果显示,BH07株基因全长1 767 bp,BH11株全长为1 766 bp,它们与天津的AY181946株同源性分别为97.6%、97.8%;通过与6个不同基因亚型的8个参考株的猪圆环病毒全基因组序列进化分析,发现BH07株和BH11株均为PCV2d亚型。
Abstract:
-

参考文献/References:

[1]Grau-Roma L,Fraile L,Segalés J. Recent advances in the epidemiology,diagnosis and control of diseases caused by porcine circovirus type 2[J]. Veterinary Journal,2011,187(1):23-32.
[2]Gillespie J,Opriessnig T,Meng X J,et al. Porcine circovirus type 2 and porcine circovirus-associated disease[J]. Journal of Veterinary Internal Medicine,2009,23(6):1151-1163.
[3]Opriessnig T,Meng X J,Halbur P G. Porcine circoviruses type 2 associated disease:update on current terminology,clinical manifestations,pathogenesis,diagnosis,and intervention strategies[J]. J Vet Diagn Investig,2007,19:591-615.
[4]Wang F,Guo X,Ge X,et al. Genetic variation analysis of Chinese strains of porcine circovius type 2[J]. Virus Research,2009,145(1):151-156.
[5]李俊,时建立,于周,等. 猪圆环病毒2型双拷贝感染性DNA的构建及体外拯救[J]. 生物工程学报,2009,25(11):1633-1638.
[6]Wang F,Guo X,Ge X,et al. Genetic variation analysis of Chinese strains of porcine circovirus type 2[J]. Virus Research,2009,145(1):151-156.
[7]曹东阳,王小敏,钱爱东,等. 江苏省及周边地区猪圆环病毒Ⅱ型(PCV2)分子流行病学调查[J]. 江苏农业学报,2016,32(2):390-398.

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备注/Memo

备注/Memo:
收稿日期:2016-10-14
基金项目:横向课题(编号:00010115009);江苏农牧科技学院院级课题(编号:NSFPT201511、NSFPT201604);江苏省泰州市农业支撑项目(编号:TN201423)。
作者简介:陶宏卫(1976—),男,江苏泰州人,硕士,讲师,从事动物医学类教学及科研工作。E-mail:2260973045@qq.com。
通信作者:郭广富,硕士,讲师,从事动物医学类教学及科研工作,E-mail:ggf66@sina.com;曹军平,博士,教授,从事动物医学类教学、科研及社会服务工作,E-mail:cccc62@163.com。
更新日期/Last Update: 2018-08-20