|本期目录/Table of Contents|

[1]陈光哲,曾德新,周昊,等.PMA在副溶血性弧菌检测中的应用[J].江苏农业科学,2019,47(04):162-167.
 Chen Guangzhe,et al.Application of PMA in detection of Vibrio parahaemolyticus[J].Jiangsu Agricultural Sciences,2019,47(04):162-167.
点击复制

PMA在副溶血性弧菌检测中的应用(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第04期
页码:
162-167
栏目:
贮藏加工与检测分析
出版日期:
2019-03-15

文章信息/Info

Title:
Application of PMA in detection of Vibrio parahaemolyticus
作者:
陈光哲1 曾德新2 周昊1 谢青轩1 沈嘉敏1
1.江苏省农业科学院中心实验室,江苏南京 210014; 2.南京海关检验检疫动植物与食品检测中心,江苏南京 210019
Author(s):
Chen Guangzheet al
关键词:
副溶血性弧菌活菌检测食源性病原菌叠氮溴化丙锭(PMA)
Keywords:
-
分类号:
TS207.4
DOI:
-
文献标志码:
A
摘要:
在我国,副溶血性弧菌被认为是海产品衍生疾病的主要因素,因此急需一种能快速、灵敏、特异、准确地检测食品中副溶血性弧菌的方法。传统培养法一直是检测的金标准,但是其检测周期较长;分子生物学方法如聚合酶链式反应(PCR)、实时荧光定量PCR(qPCR)、环介导等温扩增技术(LAMP)已被广泛用于食品中副溶血性弧菌的检测,但是这些方法不能区分死菌和活菌的DNA,容易造成假阳性。而叠氮溴化丙锭(propidium monoazide,简称PMA)等核酸染料的使用可以有效地消除这一弊端。研究者们将PMA处理与PCR(qPCR)结合,应用于食品中多种食源性致病菌活菌的检测,但是关于PMA在副溶血性弧菌检测中应用的报道并不多见。因此,拟对PMA应用于食品中副溶血性弧菌检测的可行性进行分析,以便为进一步开展相关研究提供参考。
Abstract:
-

参考文献/References:

[1]Shen X S,Cai Y Q,Liu C C,et al. Effect of temperature on uptake and survival of Vibrio parahaemolyticus in oysters (Crassostrea plicatula)[J]. International Journal of Food Microbiology,2009,136(1):129-132.
[2]Lin Y T,Labbe R G,Shetty K. Inhibition of Vibrio parahaemolyticus in seafood systems using oregano and cranberry phytochemical synergies and lactic acid[J]. Food Sci Emerg,2005,6(4):453-458.
[3]Honda T,Iida T. The pathogenicity of Vibrio parahaemolyticus and the role of thermostable direct haemolysin and related haemolysins[J]. Rev Med Microbiol,1993,4(2):106-113.
[4]Honda S,Goto I,Minematsu I,et al. Vibrio parahaemolyticus infectious disease caused by Kanagawa phenomenon-negative O3 ∶K6 originated from Maldives[J]. Kansenshoqaku Zasshi,1987,61(9):1070-1078.
[5]Honda S,Goto I,Minematsu I,et al. Gastroenteritis due to Kanagawa negative Vibrio parahaemolyticus[J]. The Lancet,1987,29(37):331-332.
[6]郭钦,陈会,徐海堂,等. 副溶血弧菌快速检测技术研究进展[J]. 食品工业科技,2016,37(24):395-400.
[7]Kim H J,Lee H J,Lee K H,et al. Simultaneous detection of pathogenic Vibrio species using multiplex real-time PCR[J]. Food Control,2012,23(2):491-498.
[8]Garrido A,Chapela M J,Román B,et al. A new multiplex real-time PCR developed method for Salmonella spp. and Listeria monocytogenes detection in food and environmental samples[J]. Food Control,2013,30(1):76-85.
[9]Ma K,Deng Y,Bai Y,et al. Rapid and simultaneous detection of Salmonella,Shigella,and Staphylococcus aureus in fresh pork using a multiplex real-time PCR assay based on immunomagnetic separation[J]. Food Control,2014,42:87-93.
[10]Zhang Z H,Xiao L L,Lou Y,et al. Development of a multiplex real-time PCR method for simultaneous detection of Vibrio parahaemolyticus,Listeria monocytogenes and Salmonella spp. in raw shrimp[J]. Food Control,2015,51:31-36.
[11]Martinon A,Cronin U P,Quealy J,et al. Swab sample preparation and viable real-time PCR methodologies for the recovery of Escherichia coli,Staphylococcus aureus or Listeria monocytogenes from artificially contaminated food processing surfaces[J]. Food Control,2012,24(1/2):86-94.
[12]Nocker A,Sossa K E,Camper A K. Molecular monitoring of disinfection efficacy using propidium monoazide in combination with quantitative PCR[J]. Journal of Microbiological Methods,2007,70(2):252-260.
[13]Pan Y,Breidt J . Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells[J]. Applied and Environmental Microbiology,2007,73(24):8028-8031.
[14]Elizaquível P,Sánchez G,Selma M V,et al. Application of propidium monoazide-qPCR to evaluate the ultrasonic inactivation of Escherichia coli O157 ∶H7 in fresh-cut vegetable wash water[J]. Food Microbiol,2012,30(1):316-320.
[15]Josefsen M H,Lfstrm C,Hansen T B,et al. Rapid quantification of viable Campylobacter bacteria on chicken carcasses,using real-time PCR and propidium monoazide treatment,as a tool for quantitative risk assessment[J]. Applied and Environmental Microbiology,2010,76(15):5097-5104.
[16]Martinon A,Cronin U P,Wilkinson M G. Development of defined microbial population standards using fluorescence activated cell sorting for the absolute quantification of S.aureus using real-time PCR[J]. Mol Biotechnol,2012,50(1):62-71.
[17]Singh G,Vajpayee P,Bhatti S,et al. Determination of viable Salmonellae from potable and source water through PMA assisted qPCR[J]. Ecotoxicology and Environmental Safety,2013,93(1):121-127.
[18]Letchumanan V,Chan K G,Lee L H. Vibrio parahaemolyticus:a review on the pathogenesis,prevalence,and advance molecular identification techniques[J]. Frontiers in Microbiology,2014,5(705):1-13.
[19]Malcolm T T H,Cheah Y K,Radzi C W J W M,et al. Detection and quantification of pathogenic Vibrio parahaemolyticus in shellfish by using multiplex PCR and loop-mediated isothermal amplification assay[J]. Food Control,2015,47:664-671.
[20]Zhan X W,Zheng Q Y,Fu J F,et al. A rapid multiplex PCR-DHPLC method of detection and identification of pathogenic bacteria in aquatic products[J]. Journal of Food Safety,2015,35(1):50-58.
[21]Zhong Q P,Dong Y Q,Wang L,et al. Development of IgY-based sandwich enzyme-linked immunosorbent assay for the detection of Vibrio parahaemolyticus[J]. Information Tech and Agricultural Eng,2012,134:517-524.
[22]王报贵,王广峰,武晓丽,等. 副溶血弧菌胶体金检测试纸条的改进[J]. 食品工业科技,2014,34(10):57-61,65.
[23]孔繁德,刘阳,徐淑菲,等. 免疫金层析技术快速检测副溶血弧菌方法的初步研究[J]. 中国兽医科学,2012,41(8):819-824.
[24]Teng J,Ye Y W,Yao L,et al. Rolling circle amplification based amperometric aptamer/immuno hybrid biosensor for ultrasensitive detection of Vibrio parahaemolyticus[J]. Microchimica Acta,2017,184(9):3477-3485.
[25]李春凤,赵勇,王晓英,等. 基于上转发光免疫层析技术的常见食源性致病菌快速检测方法研究与评价[J]. 军事医学,2015(2):128-132.
[26]McIngvale S C,Elhanafi D,Drake M A. Optimization of reverse transcriptase PCR to detect viable Shiga-toxin-producing Escherichia coli[J]. Appl Environ Microbiol,2002,68(2):799-806.
[27]Yaron S,Matthews K R. A reverse transcriptase-polymerase chain reaction assay for detection of viable Escherichia coli O157 ∶H7:investigation of specific target genes[J]. Journal of Applied Microbiology,2002,92(4):633-640.
[28]Klein P G,Juneja V K. Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR[J]. Appl Environ Microbiol,1997,63(11):4441-4448.
[29]Sheridan G E,Masters C I,Shallcross J A,et al. Detection of mRNA by reverse transcription PCR as an indicator of viability in Escherichia coli cells[J]. Applied and Environmental Microbiology,1998,64(4):1313-1318.
[30]Szabo E A,Mackey B M. Detection of salmonella enteritidis by reverse transcription-polymerase chain reaction (PCR)[J]. International Journal of Food Microbiology,1999,51(2/3):113-122.
[31]de Wet S C,Denman S E,Sly L,et al. An improved method for RNA extraction from carcass samples for detection of viable Escherichia coli O157 ∶H7 by reverse-transcriptase polymerase chain reaction[J]. Letters in Applied Microbiology,2008,47(5):399-404.
[32]DSouza D H,Critzer F J,Golden D A. Real-timereverse-transcriptase polymerase chain reaction for the rapid detection of Salmonella using inv A primers[J]. Foodborne Pathog Dis,2009,6:1097-1106.
[33]Miller N D,Draughon F A,DSouza D H. Real-time reverse-transcriptase-polymerase chain reaction for Salmonella enterica detection from jalapeno and serrano peppers[J]. Foodborne Pathogens and Disease,2010,7(4):367-373.
[34]Techathuvanan C,Draughon F A,D Souza D H. Real-time reverse transcriptase PCR for the rapid and sensitive detection of Salmonella typhimurium from pork[J]. Food Prot,2010,73(3):507-514.
[35]Kurakawa T,Kubota H,Tsuji H,et al. Development of a sensitiver RNA-targeted reverse transcription-quantitative polymerase chain reaction for detection of Vibrio cholerae/mimicus,V. parahaemolyticus/alginolyticus and Campylobacter jejuni/coli[J]. Microbiol Immunol,2012,56(1):10-20.
[36]Zhou M,Yang J,Zhou X,et al. Development of a sigDE-based real-time reverse-transcriptase PCR for the detection of viable Salmonellae nterica[J]. Foodborne Pathog Dis,2014,11(7):537-544.
[37]Postollec F,Falentin H,Pavan S A,et al. Recent advances in quantitative PCR (qPCR) applications in food microbiology[J]. Food Microbiology,2011,28(5):848-861.
[38]Sung K,Hiett K L,Stern N J. Heat-treated Campylobacter and mRNA stability as determined by reverse transcriptase-polymerase chain reaction[J]. Foodborne Pathogens and Disease,2005,2(2):130-137.
[39]Xiao L L,Zhang L,Wang H H. Critical issues in detecting viable Listeria monocytogenes cells by real-time reverse transcriptase PCR[J]. Journal of Food Protection,2012,75(3):512-517.
[40]Nocker A,Cheung C Y,Camper A K. Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells[J]. Journal of Microbiological Methods,2006,67(2):310-320.
[41]Zi C,Zeng D X,Ling N,et al. An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCR[J]. BMC Microbiology,2018,18:132.
[42]Zhu R G,Li T P,Jia Y F,et al. Quantitative study of viable Vibrio parahaemolyticus cells in raw seafood using propidium monoazide in combination with quantitative PCR[J]. Journal of Microbiological Methods,2012,90(3):262-266.
[43]Li H,Xin H,Li S F. Multiplex PMA-qPCR assay with internal amplification control for simultaneous detection of viable Legionella pneumophila,Salmonella typhimurium,and Staphylococcus aureus in environmental waters[J]. Environ Sci Technol,2015,49(24):14249-14256.
[44]Wu G P,Chen S H,Levin R E. Application of ethidium bromide monoazide for quantification of viable and dead cells of Salmonella enterica by real-time loop-mediated isothermal amplification[J]. Journal of Microbiological Methods,2015,117:41-48.
[45]Zhang Z,Liu W,Xu H,et al. Propidium monoazide combined with real-time PCR for selective detection of viable Staphylococcus aureus in milk powder and meat products[J]. Journal of Dairy Science,2015,98(3):1625-1633.
[46]Soejima T,Minami J I,Xiao J Z,et al. Innovative use of platinum compounds to selectively detect live microorganisms by polymerase chain reaction[J]. Biotechnology and Bioengineering,2016,113(2):301-310.
[47]Cawthorn D M,Witthuhn R C. Selective PCR detection of viable Enterobacter sakazakii cells utilizing propidium monoazide or ethidium bromide monoazide[J]. Journal of Applied Microbiology,2008,105(4):1178-1185.
[48]van Frankenhuyzen J K,Trevors J T,Lee H A,et al. Molecular pathogen detection in biosolids with a focus on quantitative PCR using propidium monoazide for viable cell enumeration[J]. Journal of Microbiological Methods,2011,87(3):263-272.
[49]Li B,Chen J Q. Real-time PCR methodology for selective detection of viable Escherichia coli O157 ∶H7 cells by targeting Z3276 as a genetic marker[J]. Appl Environ Microbiol,2012,78(15):5297-5304.
[50]Liu Y R,Mustapha A. Detection of viable Escherichia coli O157 ∶H7 in ground beef by propidium monoazide real-time PCR[J]. International Journal of Food Microbiology,2014,170:48-54.
[51]Dinu L D,Bach S. Detection of viable but non-culturable Escherichia coli O157 ∶H7 from vegetable samples using quantitative PCR with propidium monoazide and immunological assays[J]. Food Control,2013,31(2):268-273.
[52]Li B,Chen J Q. Development of a sensitive and specific qPCR assay in conjunction with propidium monoazide for enhanced detection of live Salmonella spp. in food[J]. BMC Microbiology,2013,13:273.
[53]Barbau-Piednoir E,Mahillon J,Pillyser J,et al. Evaluation of viability-qPCR detection system on viable and dead Salmonella serovar Enteritidis[J]. Microbiol Methods,2014,103:131-137.
[54]Elizaquível P,Sánchez G,Aznar R. Application of propidium monoazide quantitative PCR for selective detection of live Escherichia coli O157 ∶H7 in vegetables after inactivation by essential oils[J]. International Journal of Food Microbiology,2012,159(2):115-121.
[55]Banihashemi A,Van Dyke M I,Huck P M. Long-amplicon propidium monoazide-PCR enumeration assay to detect viable Campylobacter and Salmonella[J]. Appl Microbiol,2012,113(4):863-873.
[56]康昌源,王庆奎,王静波,等. 肉桂醛脂质体对3种水产动物致病菌抑菌效果比较[J]. 江苏农业科学,2018,46(18):176-178.
[57]吕孙建,刘莉,曹铮,等. 一株中华鳖气单胞菌噬菌体的分离及功能鉴定[J]. 江苏农业科学,2018,46(2):108-111.
[58]Zhang Z H,Liu H Q,Lou Y,et al. Quantifying viable Vibrio parahaemolyticus and Listeria monocytogenes simultaneously in raw shrimp[J]. Applied Microbiology and Biotechnology,2015,99(15):6451-6462.
[59]Luo J F,Lin W T,Guo Y. Method to detect only viable cells in microbial ecology[J]. Applied Microbiology and Biotechnology,2010,86(1):377-384.
[60]Schnetzinger F,Pan Y,Nocker A. Use of propidium monoazide and increased amplicon length reduce false-positive signals in quantitative PCR for bioburden analysis[J]. Applied Microbiology and Biotechnology,2013,97(5):2153-2162.
[61]Pacholewicz E,Swart A,Lipman L J,et al. Propidium monoazide does not fully inhibit the detection of dead Campylobacter on broiler chicken carcasses by qPCR[J]. Microbiol Methods,2013,95(1):32-38.
[62]Duarte-Guevara P,Duarte-Guevara C,Ornob A A. On-chip PMA labeling of foodborne pathogenic bacteria for viable qPCR and qLAMP detection[J]. Microfluidics and Nanofluidics,2016,20(8):114.

相似文献/References:

[1]高玮,金沁,赵冉.贝类产品中副溶血性弧菌的污染状况调查[J].江苏农业科学,2014,42(02):258.
 Gao Wei,et al.Investigation of pollution of Vibrio parahaemolyticus in shellfish products[J].Jiangsu Agricultural Sciences,2014,42(04):258.
[2]刘建欣,刘蕾,郭珊珊,等.副溶血性弧菌外膜蛋白BamA重组表达及其免疫原性分析[J].江苏农业科学,2022,50(15):43.
 Liu Jianxin,et al.Recombinant expression and immunogenicity of outer membrane protein BamA of Vibrio parahaemolyticus[J].Jiangsu Agricultural Sciences,2022,50(04):43.

备注/Memo

备注/Memo:
收稿日期:2018-10-19
作者简介:陈光哲(1965—),女,江苏南通人,副研究员,主要研究方向为食品农产品检测技术及方法。E-mail:13813841207@126.com。
更新日期/Last Update: 2019-02-20