[1]张蕾,郝婧玮,宛春雷,等.桦褐孔菌多糖的提取工艺优化及对人肝癌细胞HepG2脂肪堆积的影响[J].江苏农业科学,2019,47(10):201-204.
Zhang Lei,et al.Optimization of extraction process of polysaccharide from Phaeoporus obliquus and its effect on fat accumulation in HepG2 cells[J].Jiangsu Agricultural Sciences,2019,47(10):201-204.
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桦褐孔菌多糖的提取工艺优化
及对人肝癌细胞HepG2脂肪堆积的影响(PDF)
Zhang Lei,et al.Optimization of extraction process of polysaccharide from Phaeoporus obliquus and its effect on fat accumulation in HepG2 cells[J].Jiangsu Agricultural Sciences,2019,47(10):201-204.
及对人肝癌细胞HepG2脂肪堆积的影响(PDF)
《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]
- 卷:
- 第47卷
- 期数:
- 2019年第10期
- 页码:
- 201-204
- 栏目:
- 贮藏加工与检测分析
- 出版日期:
- 2019-06-12
文章信息/Info
- Title:
- Optimization of extraction process of polysaccharide from Phaeoporus obliquus and its effect on fat accumulation in HepG2 cells
- Keywords:
- -
- 分类号:
- R284
- DOI:
- -
- 文献标志码:
- A
- 摘要:
- 通过L9(34)正交试验，通过MTT法选取桦褐孔菌多糖对HepG2细胞的安全浓度，检测不同浓度的桦褐孔菌多糖对胞内TG蛋白的影响，及桦褐孔菌多糖减轻细胞内脂质堆积的作用。结果表明，桦褐孔菌多糖最佳条件为 1 g ∶ 15 mL 的料液比，在温度30 ℃下提取30 min，超声功率为480 W。桦褐孔菌多糖可明显减轻HepG2细胞内脂质堆积，显著降低细胞中TG的含量，其中600 mg/L桦褐孔菌多糖对TG的清除率达24.57%，脂滴数量明显减少。说明桦褐孔菌多糖具有良好的体外降脂活性，对脂肪肝有较好的预防效果。
- Abstract:
- -
参考文献/References:
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备注/Memo
- 备注/Memo:
-
收稿日期：2018-11-03
基金项目：黑龙江省教育厅科研备案项目(编号：1352MSYQN002)；黑龙江省大学生创新创业训练计划(编号：201810233045)；黑龙江省牡丹江市科学技术计划(编号：Z2016s0024)。
作者简介：张蕾(1984—)，女，黑龙江东宁人，博士，讲师，主要从事微生物与生物化学研究。E-mail：swxzlz@126.com。
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