|本期目录/Table of Contents|

[1]徐婷,薛金嫚,陈亚东,等.一种简易检测6-磷酸葡萄糖的方法[J].江苏农业科学,2019,47(10):209-212.
　Xu Ting,et al.A simple method for detection of glucose-6-phosphate[J].Jiangsu Agricultural Sciences,2019,47(10):209-212.
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一种简易检测6-磷酸葡萄糖的方法(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第10期
页码:
209-212
栏目:
贮藏加工与检测分析
出版日期:
2019-06-12

文章信息/Info

Title:
A simple method for detection of glucose-6-phosphate
作者:
徐婷;  薛金嫚;  陈亚东;  刘华;  陈钰佩
浙江农林大学农业与食品科学学院，浙江杭州 311300
Author(s):
Xu Ting; et al
关键词:
6-磷酸葡萄糖; 高效液相色谱; 酶学荧光检测; 酵母
Keywords:
-
分类号:
Q532+.5
DOI:
-
文献标志码:
A
摘要:
6-磷酸葡萄糖(G6P)是多种代谢途径的关键中间产物，一种简易高效的G6P测定法建立将有利于深入了解其在有机体中的代谢状态。前期研究发现，基于离子排斥色谱柱Bio-Rad Aminex HPX-87H与示差折光检测器的高效液相色谱分析无法有效地将这一化合物与1，6-二磷酸果糖、3-磷酸甘油酸区分开来。因而，拟建立一种简便、专一且灵敏，检测浓度范围为0.2~10 μmol/L的G6P的酶学荧光检测法。同时，我们以模式真核生物酵母为材料，对G6P的提取方法进行改进，以使样本的测定更加稳定。
Abstract:
-

参考文献/References:

［1］Legras J L，Merdinoglu D，Cornuet J M，et al. Saccharomyces cerevisiae diversity reflects human history［J］. Molecular Ecology，2007，16(10)：2091-2102.
［2］于定群，汤浩茹，张勇，等. 高等植物葡萄糖-6-磷酸脱氢酶的研究进展［J］. 生物工程学报，2012，28(7)：800-812.
［3］Zhu A P，Romero R，Petty H R. An enzymatic fluorimetric assay for glucose-6-phosphate：application in an in vitro Warburg-like effect［J］. Analytical Biochemistry，2009，388(1)：97-101.
［4］Ritter J B，Genzel Y，Reichl U. Simultaneous extraction of several metabolites of energy metabolism and related substances in mammalian cells：optimization using experimental design［J］. Analytical Biochemistry，2008，373(2)：349-369.
［5］Buchholz A，Takors R，Wandrey C. Quantification of intracellular metabolites in Escherichia coli K12 using liquid chromatographic-electrospray ionization tandem mass spectrometric techniques［J］. Analytical Biochemistry，2001，295(2)：129-137.
［6］van Dam J C，Eman M R，Frank J，et al. Analysis of glycolytic intermediates in Saccharomyces cerevisiae using anion exchange chromatography and electrospray ionization with tandem mass spectrometric detection［J］. Analytica Chimica Acta，2002，460(2)：209-218.
［7］Bhattacharya M，Fuhrman L，Ingram A，et al. Single-run separation and detection of multiple metabolic intermediates by anion-exchange high-performance liquid chromatography and application to cell pool extracts prepared from Escherichia coli［J］. Analytical Biochemistry，1995，232(1)：98-106.
［8］杨海燕，法芸. 离子排斥色谱法同时测定生物质液体燃料发酵液中的有机酸、糖、醇［J］. 化学分析计量，2014，23(增刊1)：13-15.
［9］Antonio C，Larson T，Gilday A，et al. Quantification of sugars and sugar phosphates in Arabidopsis thaliana tissues using porous graphitic carbon liquid chromatography-electrospray ionization mass spectrometry［J］. Journal of Chromatography A，2007，1172(2)：170-178.
［10］陈洁梅，潘涛，陈星旦. 二阶导数光谱预处理在用FTIR/ATR方法定量测定葡萄糖-6-磷酸和果糖-6-磷酸中的应用［J］. 光学精密工程，2006，14(1)：1-7.
［11］Gibon Y，Vigeolas H，Tiessen A，et al. Sensitive and high throughput metabolite assays for inorganic pyrophosphate，ADPGlc，nucleotide phosphates and glycolytic intermediates based on a novel enzymic cycling system［J］. Plant Journal，2002，30(2)：221-235.
［12］Self C H. Enzyme amplification—A general method applied to provide an immunoassisted assay for placental alkaline phosphatase［J］. Journal of Immunological Methods，1985，76(2)：389-393.
［13］Johansson A，Ellis D H，Bates D L，et al. Enzyme amplification for immunoassays - detection limit of 100th of an attomole［J］. Journal of Immunological Methods，1986，87(1)：7-11.
［14］De Jong D W. Woodlief W G.fluorimetric assay of tobacco leaf dehydrogenases with resazurin［J］. Biochimica Et Biophysica Acta，1977，484(2)：249-259.
［15］Batchelor R H，Zhou M. Use of cellular glucose-6-phosphate dehydrogenase for cell quantitation：applications in cytotoxicity and apoptosis assays［J］. Analytical Biochemistry，2004，329(1)：35-42.

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备注/Memo

备注/Memo:
收稿日期：2018-01-08
基金项目：国家自然科学基金(编号：31370287)。
作者简介：徐婷(1992—)，女，安徽马鞍山人，硕士研究生，主要从事油脂代谢分子生物学和生物化学研究。E-mail：1209250518@qq.com。
通信作者：陈钰佩，硕士，主要从事油脂代谢分子生物学和生物化学研究。E-mail：shuiweipei2014@163.com。
更新日期/Last Update: 2019-05-20