|本期目录/Table of Contents|

[1]余艳玲,彭昊,冯世文,等.罗非鱼无乳链球菌环介导等温扩增(LAMP)检测技术的建立及应用[J].江苏农业科学,2019,47(13):200-203.
　Yu Yanling,et al.Establishment and application of real-time loop-mediated isothermal amplification techniques for detection of Streptococcus agalactiae in tilapia[J].Jiangsu Agricultural Sciences,2019,47(13):200-203.
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罗非鱼无乳链球菌环介导等温扩增(LAMP)
检测技术的建立及应用(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第13期
页码:
200-203
栏目:
畜牧兽医与水产蚕桑
出版日期:
2019-07-31

文章信息/Info

Title:
Establishment and application of real-time loop-mediated isothermal amplification techniques for detection of Streptococcus agalactiae in tilapia
作者:
余艳玲1;  彭昊2;  冯世文2;  张永德1;  罗洪林1;  李军2
1.广西水产科学研究院/广西水产遗传育种与健康养殖重点实验室，广西南宁 530021；
2.广西兽医研究所/广西兽医生物技术重点实验室，广西南宁 530001
Author(s):
Yu Yanling; et al
关键词:
罗非鱼; 无乳链球菌(GBS); 环介导等温扩增(LAMP)
Keywords:
-
分类号:
S917
DOI:
-
文献标志码:
A
摘要:
针对罗非鱼无乳链球菌Sip基因建立了LAMP检测方法，并对野外样本进行了无乳链球菌的调查研究。结果表明，目标Sip基因可在63 ℃ 40 min内被LAMP检测到，比PCR快约2 h，其DNA检测最低浓度为3.55×10-5 ng/μL，灵敏度比PCR高100倍，且对参考细菌无扩增。野外样品检测结果表明，运用LAMP检测技术对GBS的检出率为813%，比PCR高约20.0%。应用LAMP和PCR分别检测健康罗非鱼GBS检出率4.4%和2.2%，检测池塘水样品GBS检出率分别为10.0%和6.7%。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2018-04-08
基金项目：国家自然科学基金面上项目(编号：31372553)；广西自然科学基金(编号：2015GXNSFAA139068)。
作者简介：余艳玲(1976—)，女，陕西咸阳人，硕士，工程师，主要从事水产动物分子生物学研究。E-mail：yaeling@126.com。
通信作者：李军，副研究员，主要从事动物疫病防控与病原分子生物学研究工作。E-mail：jlee9981@163.com。
更新日期/Last Update: 2019-07-05