|本期目录/Table of Contents|

[1]刘小莉,王荣,王帆,等.温度和pH值条件对白鱼内源性蛋白酶活性的影响[J].江苏农业科学,2020,48(2):188-192.
 Liu Xiaoli,et al.Effects of temperature and pH value on activity of endogenous proteases in Culter alburnus[J].Jiangsu Agricultural Sciences,2020,48(2):188-192.
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温度和pH值条件对白鱼内源性蛋白酶活性的影响(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年第2期
页码:
188-192
栏目:
贮藏加工与检测分析
出版日期:
2020-03-05

文章信息/Info

Title:
Effects of temperature and pH value on activity of endogenous proteases in Culter alburnus
作者:
刘小莉1王荣2王帆1崔晋1周剑忠1
1.江苏省农业科学院农产品加工研究所,江苏南京 210014; 2.泰兴市江之韵科技发展有限公司,江苏泰兴 225400
Author(s):
Liu Xiaoliet al
关键词:
白鱼温度pH值内源酶肌原纤维蛋白
Keywords:
-
分类号:
TS254.4
DOI:
-
文献标志码:
A
摘要:
研究不同温度和pH值条件对白鱼内源性蛋白酶活性及其降解肌原纤维蛋白的影响。结果表明,钙蛋白酶残余活性在不同温度和pH值条件下差异较大,在温度为4、10、20、37 ℃条件下放置20 h后残余活性分别为94.5%、63.2%、43.9%、47.1%;在pH值为4.50、5.00、5.50、6.89条件下放置20 h后残余活性分别为94.62%、77.86%、3136%、60.04%。在不同温度和pH值条件下组织蛋白酶D变化幅度次于钙蛋白酶,而组织蛋白酶B、L残余活性的差异不大。SDS-PAGE结果显示,随着温度的升高,白鱼肌肉肌球蛋白重链降解程度增加,而在pH值为5.00、5.50条件下肌球蛋白重链和肌动蛋白的降解均很明显。综合考虑生产实际,最佳操作环境温度为10~20 ℃、腌制液pH值为4.5。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2018-10-10
基金项目:江苏省水产三新工程(编号:Y2015-29);江苏省泰州市高层次创新创业人才(团队)引进计划。
作者简介:刘小莉(1981—),女,江苏泰兴人,博士,研究员,研究方向为水产品加工。E-mail:liuxljaas@hotmail.com。
通信作者:周剑忠,博士,研究员,研究方向为水产品加工。E-mail:zjzluck@126.com。
更新日期/Last Update: 2020-01-20