|本期目录/Table of Contents|

[1]李明,丁丽娜,王政,等.甘蓝型油菜脂肪酶基因BnGLIP1的克隆与分析[J].江苏农业科学,2020,48(04):59-65.
　Li Ming,et al.Cloning and expression analysis of lipase gene BnGLIP1 in Brassica napus L.[J].Jiangsu Agricultural Sciences,2020,48(04):59-65.
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甘蓝型油菜脂肪酶基因BnGLIP1的克隆与分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年第04期
页码:
59-65
栏目:
生物技术
出版日期:
2020-03-25

文章信息/Info

Title:
Cloning and expression analysis of lipase gene BnGLIP1 in Brassica napus L.
作者:
李明1;  丁丽娜1;  王政1;  曹维1;  王玉康1;  张洁夫2;  谭小力1
1.江苏大学生命科学研究院，江苏镇江 212013； 2.江苏省农业科学院经济作物研究所，江苏南京 210014
Author(s):
Li Ming; et al
关键词:
甘蓝型油菜; BnGLIP1; 生物信息学; 表达模式分析; 胁迫响应
Keywords:
-
分类号:
S634.301；Q785
DOI:
-
文献标志码:
A
摘要:
植物GDSL脂肪酶是一个重要的脂肪酶家族，具有水解酶活性，在植物体内参与众多的生理活动。从甘蓝型油菜中克隆到1个GDSL类型脂肪酶基因，将其命名为BnGLIP1。该脂肪酶基因编码的蛋白有360个氨基酸，含有20种氨基酸，分子质量为39 545.35 u，理论等电点为4.95。生物信息学分析表明，该蛋白属于稳定性蛋白，可能为亲水性蛋白；编码蛋白的N端具有信号肽序列，推测可能为外分泌蛋白；蛋白质二级和三级结构显示α螺旋和无规则卷曲所占比例较高。组织特异性表达分析表明，BnGLIP1在甘蓝型油菜的根、茎、茎尖、叶、花、角果、种子等中均有表达，其中角果中的表达量最高，茎中最低。干旱、高盐等胁迫处理均能诱导BnGLIP1的表达，表明BnGLIP1脂肪酶基因在植物的胁迫响应中发挥作用。
Abstract:
-

参考文献/References:

［1］解蕊. 一种潜在的植物蛋白资源——菜籽蛋白的开发［J］. 农产品加工，2003(1)：26-27.
［2］王汉中. 发展油菜生物柴油的潜力、问题与对策［J］. 中国油料作物学报，2005，27(2)：74-76.
［3］Lai C P，Huang L M，Chen L O，et al. Genome-wide analysis of GDSL-type esterases/lipases in Arabidopsis［J］. Plant Molecular Biology，2017，95(1/2)：181-197.
［4］Chepyshko H，Lai C P，Huang L M，et al. Multifunctionality and diversity of GDSL esterase/lipase gene family in rice (Oryza sativa L. japonica) genome：new insights from bioinformatics analysis［J］. BMC Genomics，2012，13(1)：309.
［5］Volokita M，Rosilio-Brami T，Rivkin N，et al. Combining comparative sequence and genomic data to ascertain phylogenetic relationships and explore the evolution of the large GDSL-lipase family in land plants［J］. Molecular Biology and Evolution，2011，28(1)：551-565.
［6］Akoh C C，Lee G C，Liaw Y C，et al. GDSL family of serine esterases/lipases［J］. Progress in Lipid Research，2004，43(6)：534-552.
［7］Ji R，Wang H，Xin X，et al. BrEXL6，a GDSL lipase gene of Brassica rapa，functions in pollen development［J］. Biologia Plantarum，2017，61(4)：685-692.
［8］Ma R，Yuan H，An J，et al. A Gossypium hirsutum GDSL lipase/hydrolase gene (GhGLIP) appears to be involved in promoting seed growth in Arabidopsis［J］. PLoS One，2018，13(4)：e0195556.
［9］Kwon S J，Jin H C，Lee S，et al. GDSL lipase-like 1 regulates systemic resistance associated with ethylene signaling in Arabidopsis［J］. The Plant Journal，2009，58(2)：235-245.
［10］Zhang Y J，Bai B，Lee M，et al. Cloning and characterization of EgGDSL，a gene associated with oil content in oil palm［J］. Scientific Reports，2018，8(1)：11406.
［11］Ling H，Zuo K，Zhao J，et al. Isolation and characterization of a homologous to lipase gene from Brassica napus［J］. Russian Journal of Plant Physiology，2006，53(3)：366-372.
［12］Takahashi K，Shimada T，Kondo M，et al. Ectopic expression of an esterase，which is a candidate for the unidentified plant cutinase，causes cuticular defects in Arabidopsis thaliana［J］. Plant & Cell Physiology，2010，51(1)：123-131.
［13］Kim H G，Kwon S J，Jang Y J，et al. GDSL lipase 1 regulates ethylene signaling and ethylene-associated systemic immunity in Arabidopsis［J］. FEBS Letters，2014，588(9)：1652-1658.
［14］Ding L N，Guo X J，Li M，et al. Improving seed germination and oil contents by regulating the GDSL transcriptional level in Brassica napus［J］. Plant Cell Reports，2019，38(2)：243-253.
［15］Chen M X，Du X，Zhu Y，et al. Seed Fatty Acid Reducer acts downstream of gibberellin signalling pathway to lower seed fatty acid storage in Arabidopsis［J］. Plant，Cell & Environment，2012，35(12)：2155-2169.
［16］郝晓云，蔡永智，袁哈利，等. 过量表达棉花GDSL脂肪酶提高甘蓝型油菜油脂含量的研究［J］. 中国粮油学报，2014，29(6)：63-68，73.
［17］Marchler-Bauer A，Derbyshire M K，Gonzales N R，et al. CDD：NCBIs conserved domain database［J］. Nucleic Acids Research，2015，43：D222-D226.
［18］Emanuelsson O，Brunak S，von Heijne G，et al. Locating proteins in the cell using TargetP，SignalP and related tools［J］. Nature Protocols，2007，2(4)：953-971.
［19］Kondou Y，Nakazawa M，Kawashima M，et al. RETARDED GROWTH OF EMBRYO1，a new basic helix-loop-helix protein，expresses in endosperm to control embryo growth［J］. Plant Physiology，2008，147(4)：1924-1935.
［20］Lee D S，Kim B K，Kwon S J，et al. Arabidopsis GDSL lipase 2 plays a role in pathogen defense via negative regulation of auxin signaling［J］. Biochemical and Biophysical Research Communications，2009，379(4)：1038-1042.
［21］Oh I S，Park A R，Bae M S，et al. Secretome analysis reveals an Arabidopsis lipase involved in defense against Alternaria brassicicola［J］. The Plant Cell，2005，17(10)：2832-2847.

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备注/Memo

备注/Memo:
收稿日期：2019-03-05
基金项目：国家重点研发计划(编号：2016YFD0100305)。
作者简介：李明(1993—)，女，山东菏泽人，硕士研究生，研究方向为模式生物功能基因组学和蛋白质组学。E-mail：LMING0309@126.com。
通信作者：谭小力，博士，研究员，研究方向为油菜功能基因组学。E-mail：xltan@ujs.edu.cn。
更新日期/Last Update: 2020-02-20