|本期目录/Table of Contents|

[1]徐海,鲍熹,董洪燕,等.T7噬菌体穿孔素的克隆表达及对宿主损伤研究[J].江苏农业科学,2020,48(24):45-49.
　Xu Hai,et al.Cloning and expression of T7 bacteriophage perforin and its damage to the host[J].Jiangsu Agricultural Sciences,2020,48(24):45-49.
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T7噬菌体穿孔素的克隆表达及对宿主损伤研究(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年第24期
页码:
45-49
栏目:
生物技术
出版日期:
2020-12-30

文章信息/Info

Title:
Cloning and expression of T7 bacteriophage perforin and its damage to the host
作者:
徐海1;  鲍熹2;  董洪燕1;  郭长明1;  邓碧华2;  卢宇2;  朱善元1
1.江苏农牧科技职业学院/江苏省兽用生物制药高技术研究重点实验室，江苏泰州 225300；2.江苏省农业科学院动物免疫工程研究所，江苏南京 210014
Author(s):
Xu Hai; et al
关键词:
穿孔素; 表达; 宿主损伤; T7噬菌体
Keywords:
-
分类号:
S188
DOI:
-
文献标志码:
A
摘要:
为研究T7噬菌体穿孔素(Holin)蛋白对宿主细菌的增殖性能的影响，根据T7噬菌体gene 17.5设计引物，PCR扩增Holin基因和序列分析，将该基因插入原核表达载体构建重组表达菌BL-Holin。IPTG诱导Holin蛋白表达，SDS-PAGE和Western-blot检测目标蛋白。分光光度法测定Holin蛋白对宿主细菌增殖性能的影响，扫描电镜观察重组菌诱导后表面结构的损伤情况。结果表明，成功扩增Holin基因，构建的重组菌能高效表达Holin蛋白。T7噬菌体Holin蛋白含有1个跨膜区，氨基端位于胞外、羧基端位于胞内。Holin蛋白的表达量随诱导时间延长而逐渐增加，细菌浓度从诱导0 h至2 h逐渐升高，2 h后细菌浓度开始下降，直至6 h，电镜观察发现诱导细菌表面塌陷、皱缩进而死亡。研究结果显示，Holin蛋白具有抑菌活性，这为进一步开发噬菌体抗菌制剂提供应用基础和技术支持。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2020-04-14
基金项目：江苏农牧科技职业学院院级课题(编号：NSF201902)。
作者简介：徐海(1982—)，男，江苏扬州人，副研究员，主要从事兽用生物制品研发工作。E-mail：hai_x@126.com。
通信作者：朱善元，博士，教授，主要从事水禽疫病研究。E-mail：jstzzsy@126.com。
更新日期/Last Update: 2020-12-20