|本期目录/Table of Contents|

[1]吕孙建,袁雪梅,张海琪,等.凡纳滨对虾养殖期间水体及肠道菌群变化研究[J].江苏农业科学,2021,49(2):125-131.
 Lü Sunjian,et al.Study on changes of water body and intestinal flora during culture of Litopenaeus vannamei[J].Jiangsu Agricultural Sciences,2021,49(2):125-131.
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凡纳滨对虾养殖期间水体及肠道菌群变化研究(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第2期
页码:
125-131
栏目:
畜牧兽医与水产蚕桑
出版日期:
2021-01-20

文章信息/Info

Title:
Study on changes of water body and intestinal flora during culture of Litopenaeus vannamei
作者:
吕孙建1 袁雪梅1 张海琪1 刘莉2 于喆1 杭小英1 施伟达1
1.农业农村部淡水渔业健康养殖重点实验室/浙江省鱼类健康与营养重点实验室/浙江省淡水水产研究所,浙江湖州 313001;2.浙江省农业科学院水生生物研究所,浙江杭州 310021
Author(s):
Lü Sunjianet al
关键词:
凡纳滨对虾微生物群γ-变形菌纲蓝藻细菌纲
Keywords:
-
分类号:
S945.1+2
DOI:
-
文献标志码:
A
摘要:
近年,由于凡纳滨对虾(Litopenaeus vannamei)养殖业疾病频发,且病原种类复杂多样,对对虾养殖业造成巨大的影响,其中又以细菌病最为普遍及严重。以浙江某养殖场的6口养殖塘作为试验塘,在养殖至20 d后开始分别采用水质监测试剂盒及高通量测序等方法连续监测水质指标、水体及对虾肠道微生物群变化情况,从而了解在对虾疾病高发期水体和虾体中微生物群的动态规律。研究发现随着养殖时间延长,水体中的氨氮、硫化氢、钙离子、总氮和总磷等指标会出现显著上升。另外,养殖塘中的主要微生物群为蓝藻细菌纲和变形菌纲类细菌,且蓝藻细菌纲和γ-变形菌纲-弧菌微生物群到养殖后期数量显著上升;对虾肠道中的主要微生物群为γ-变形菌纲-弧菌,在养殖中期有明显上升。本研究首次研究了在对虾疾病高发期水体和虾体中的主要微生物群种类,为后续病害防控技术研发提供一定的理论依据。
Abstract:
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参考文献/References:

[1]Devaraja T N,Yusoff F M,Shariff M. Changes in bacterial populations and shrimp production in ponds treated with commercial microbial products[J]. Aquaculture,2002,206(3):245-256.
[2]Verschuere L,Rombaut G,Sorgeloos P,et al. Probiotic bacteria as biological control agents in aquaculture[J]. Microbiology and Molecular Biology Reviews,2000,64(4):655-671.
[3]Kong J R,Qiao X L,Yang P,et al. LvCdc42 is a potential negative regulator of Lvp53 in Litopenaeus vannamei exposed to Vibrio alginolyticus stress[J]. Developmental and Comparative Immunology,2018,82:113-117.
[4]Phupet B,Pitakpornpreecha T,Baowubon N,et al. Lipopolysaccharide-and β-1,3-glucan-binding protein from Litopenaeus vannamei:Purification,cloning and contribution in shrimp defense immunity via phenoloxidase activation[J]. Developmental and Comparative Immunology,2018,81:167-179.
[5]杨丛海,赵增元,麻次松,等. 对虾暴发性流行病综合防治技术要点[J]. 齐鲁渔业,1996,13(3):5-7.
[6]蔡莹.对虾养殖沉积环境细菌多样性的分子分析与若干可培养技术优化探讨[D]. 厦门:厦门大学,2007.
[7]胡梦红.抗生素在水产养殖中的应用、存在的问题及对策[J]. 水产科技情报,2006,33(5):217-221.
[8]孙振丽,宣引明,张皓,等. 南美白对虾养殖环境及其肠道细菌多样性分析[J]. 中国水产科学,2016,23(3):594-605.
[9]Wan L,Wang J Q,Gao F,et al. Bacterial flora in intestines of white leg shrimp(Penaeus vannamei Booen)[J]. Fisheries Science,2006,25(1):13-15.
[10]Li K,Zheng T L,Tian Y,et al. Bacterial community structure in intestine of the white shrimp,Litopenaeus vannamei[J]. Acta Microbiologica Sinica,2007,47(4):649-653.
[11]Liu H D,Wang L,Wang B J,et al. Analysis on the diversity of microorganism in intestine of Penaeus vannamei-cultured by PCR-DGGE[J]. Feed Industry,2008,29(20):55-58.
[12]王兴强,马甡,董双林.凡纳滨对虾生物学及养殖生态学研究进展[J]. 海洋湖沼通报,2004(4):94-100.
[13]杭小英,周志明,李倩,等. 不同养殖模式对南美白对虾生长、病害发生与水质的影响[J]. 江苏农业科学,2014,42(5):191-192,193.
[14]彭自然,臧维玲,高杨. 氨、亚硝酸盐对凡纳滨对虾幼虾毒性作用[J]. 上海水产大学学报,2004,13(3):274-278.
[15]孙国铭,汤建华,仲霞铭.氨氮和亚硝酸氮对南美白对虾的毒性研究[J]. 水产养殖,2002,22(1):22-24.
[16]罗鹏,胡超群,张吕平, 等. 凡纳滨对虾海水养殖系统内细菌群落的PCR-DGGE分析[J]. 中国水产科学,2009,16(1):31-38.
[17]Zeng Y,Ma Y,Wei C,et al. Bacterial diversity in various coastal mariculture ponds in southeast China and in diseased eels as revealed by culture and culture-independent molecular techniques[J]. Aquaculture Research,2010,41(9):172-186.
[18]Sakami T,Fujioka Y,Shimoda T. Comparison of microbial community structures in intensive and extensive shrimp culture ponds and a mangrove area in Thailand[J]. Fisheries Science,2008,74(4):889-898.
[19]Beardsley C,Moss S,Malfatti F,et al. Quantitative role of shrimp fecal bacteria in organic matter fluxes in a recirculating shrimp aquaculture system[J]. FEMS Microbiology Ecology,2011,77(1):134-145.
[20]Liu H D,Liu M,Wang B J,et al. PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus)[J]. Chinese Journal of Oceanology and Limnology,2010,28(4):808-814.
[21]Rungrassamee W,Klanchui A,Maibunkaew S,et al. Characterization of intestinal bacteria in wild and domesticated adult black tiger shrimp (Penaeus monodon)[J]. PLoS One,2014,9(3):e91853.
[22]Tang Y,Tao P,Tan J,et al. Identification of bacterial community composition in freshwater aquaculture system farming of Litopenaeus vannamei reveals distinct temperature-driven patterns[J]. International Journal of Molecular Sciences,2014,15(8):13663-13680.

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备注/Memo

备注/Memo:
收稿日期:2020-06-03
基金资助:浙江省科研院所扶持专项(编号:2020YSZX002、2020YSZX010)。
作者简介:吕孙建(1988—),男,浙江温州人,博士,助理研究员,主要从事水产动物病害防治。E-mail:lvsunjian@163.com。
通信作者:张海琪,硕士,正高级工程师,主要从事水产育种和病害防治。E-mail:zmk407@126.com。
更新日期/Last Update: 2021-01-20