[1]储俊东,刁澎云,李旭光,等.中华绒螯蟹表皮蛋白基因EsCAP的克隆与表达分析[J].江苏农业科学,2021,49(8):74-80.
Chu Jundong,et al.Cloning and expression analysis of cuticle protein gene EsCAP in Eriocheir sinensis[J].Jiangsu Agricultural Sciences,2021,49(8):74-80.
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中华绒螯蟹表皮蛋白基因EsCAP的克隆与表达分析(PDF)
Chu Jundong,et al.Cloning and expression analysis of cuticle protein gene EsCAP in Eriocheir sinensis[J].Jiangsu Agricultural Sciences,2021,49(8):74-80.
《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]
- 卷:
- 第49卷
- 期数:
- 2021年第8期
- 页码:
- 74-80
- 栏目:
- 生物技术
- 出版日期:
- 2021-04-20
文章信息/Info
- Title:
- Cloning and expression analysis of cuticle protein gene EsCAP in Eriocheir sinensis
- 作者:
- 储俊东1; 2; 刁澎云1; 2; 李旭光1; 2; 毕可然1; 周刚2; 周军2; 邓燕飞2; 许郑超2
- 1.江苏海洋大学，江苏连云港 222005； 2.江苏省淡水水产研究所，江苏南京 210017
- Author(s):
- Chu Jundong; et al
- Keywords:
- -
- 分类号:
- S917
- DOI:
- -
- 文献标志码:
- A
- 摘要:
- 以中华绒螯蟹(Eriocheir sinensis)为试验对象，克隆中华绒螯蟹表皮蛋白CAP基因序列，分析其表达规律，为探索中华绒螯蟹蜕皮发生机理提供参考。运用生物信息学从中华绒螯蟹转录组数据中比对筛查表皮蛋白CAP基因序列信息，克隆扩增获得表皮蛋白CAP基因cDNA序列，运用RT-qPCR分析表皮蛋白CAP基因在不同组织、不同发育阶段和不同蜕皮时期的表达特征。结果表明，中华绒螯蟹表皮蛋白CAP基因cDNA序列全长380 bp，编码101个氨基酸，包括1个信号肽和1个R&R结合域。表皮蛋白基因CAP主要在蜕皮后期的表皮组织中表达，在蜕皮间期不表达；在不同发育阶段的仔蟹1期表达量最高，根据其发育表达模式推测表皮蛋白基因CAP参与表皮的形成和钙化，为后续深入研究表皮蛋白基因的生理功能奠定基础。
- Abstract:
- -
参考文献/References:
［1］Delon I，Payre F. Evolution of larval morphology in flies：get in shape with shavenbaby［J］. Trends in Genetics，2004，20(7)：305-313.
［2］Frédéric M，Luquet G. Unusually acidic proteins in biomineralization［M］.Handbook of Biomineralization：Biological Aspects and Structure Formation，2008.
［3］Nagasawa H. The crustacean cuticle：structure，composition and mineralization［J］. Frontiers in Bioscience，2012，4(1)：711-720.
［4］Roer R，Abehsera S，Sagi A. Exoskeletons across the pancrustacea：comparative morphology，physiology，biochemistry and genetics［J］. Integrative and Comparative Biology，2015，55(5)：771-791.
［5］Andersen S O. Exoskeletal proteins from the crab，cancer pagurus［J］. Comparative Biochemistry and Physiology，1999，123(2)：203-211.
［6］郑征帆，吕艳杰，宁黔冀. 甲壳动物表皮几丁质结合蛋白结构与功能研究进展［J］. 水产科学，2017，36(4)：538-542.
［7］Andersen O S. Biochemistry of insect cuticle［J］. Annual Review of Entomology，1979，24(1)：29-59.
［8］2019年中国渔业统计年鉴［M］. 北京：中国农业出版社，2019.
［9］Andersen S O，Hjrup P，Roepstorff P. Insect cuticular proteins［J］. Insect Biochemistry and Molecular Biology，1995，25(2)：153-176.
［10］Karouzou M V，Spyropoulos Y，Iconomidou V A，et al. Drosophila cuticular proteins with the R&R consensus：annotation and classification with a new tool for discriminating RR-1 and RR-2 sequences［J］. Insect Biochemistry and Molecular Biology，2007，37(8)：754-760.
［11］Coblentz F E，Shafer T H，Roer R D. Cuticular proteins from the blue crab alter in vitro calcium carbonate mineralization［J］. Comparative Biochemistry and Physiology，1998，121(3)：349-360.
［12］Glazer L，Sagi A. On the involvement of proteins in the assembly of the crayfish gastrolith extracellular matrix［J］. Invertebrate Reproduction & Development，2012，56(1)：57-65.
［13］Endo H，Persson P，Watanabe T. Molecular cloning of the crustacean DD4 cDNA encoding a Ca2+-binding protein［J］. Biochemical and Biophysical Research Communications，2000，276(1)：286-291.
［14］Endo H，Takagi Y，Ozaki N，et al. A crustacean Ca2+-binding protein with a glutamate-rich sequence promotes CaCO3 crystallization［J］. The Biochemical Journal，2004，384(1)：159-167.
［15］Inoue H，Ohira T，Ozaki N，et al. Cloning and expression of a cDNA encoding a matrix peptide associated with calcification in the exoskeleton of the crayfish［J］. Comparative Biochemistry and Physiology(Part B Biochemistry & Molecular Biology)，2003，136(4)：755-765.
［16］Inoue H，Ohira T，Ozaki N，et al. A novel calcium-binding peptide from the cuticle of the crayfish，Procambarus clarkii［J］. Biochemical and Biophysical Research Communications，2004，318(3)：649-654.
［17］Rebers J E，Riddiford L M. Structure and expression of a Manduca sexta larval cuticle gene homologous to drosophila cuticle genes［J］. Journal of Molecular Biology，1988，203(2)：411-423.
［18］Rebers J E，Willis J H. A conserved domain in arthropod cuticular proteins binds chitin［J］. Insect Biochemistry and Molecular Biology，2001，31(11)：1083-1093.
［19］Noh M Y，Muthukrishnan S，Kramer K J，et al. Tribolium castaneum RR-1 cuticular protein TcCPR4 is required for formation of pore canals in rigid cuticle［J］. PLoS Genetics，2015，11(2)：e1004963.
［20］Mun S，Noh M Y，Dittmer N T，et al. Cuticular protein with a low complexity sequence becomes cross-linked during insect cuticle sclerotization and is required for the adult molt［J］. Scientific Reports，2015，5：10484.
［21］Abehsera S，Weil S，Manor R，et al. The search for proteins involved in the formation of crustacean cuticularstructures［J］. Hydrobiologia，2018，825(1)：29-45.
［22］Luquet G. Biomineralizations：insights and prospects from crustaceans［J］. ZooKeys，2012，176(176)：103-121.
［23］Volovych O，Lin Z，Du J，et al. Identification and temporal expression profiles of cuticular proteins in the endoparasitoid wasp，Microplitis mediator［J］. Insect Science，2020，27(5)：998-1018.
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备注/Memo
- 备注/Memo:
-
收稿日期：20200-08-11
基金项目：国家现代农业产业技术体系建设专项(编号：CARS-48)；江苏省自然科学基金面上项目(编号：BK20161602)；江苏省渔业科技类重点项目(编号：D2018-4)；江苏省农业重大新品种创制项目(编号：PZCZ201748)；江苏省现代农业(中华绒螯蟹)产业技术体系项目(编号：JATS［2019］386)；江苏省研究生科研与实践创新计划(编号：SJCX20_1269、SJCX20_1293)。
作者简介：储俊东(1996—)，男，江苏南通人，
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