|本期目录/Table of Contents|

[1]敖金成,李永梅,李博.连作烟田健株与感染根腐病烟株根际土壤细菌群落多样性研究[J].江苏农业科学,2022,50(4):198-204.
 Ao Jincheng,et al.Diversity of bacterial community in rhizosphere soil of healthy and root susceptible tobacco plants in continuous cropping tobacco fields[J].Jiangsu Agricultural Sciences,2022,50(4):198-204.
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连作烟田健株与感染根腐病烟株根际土壤细菌群落多样性研究(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第4期
页码:
198-204
栏目:
资源与环境
出版日期:
2022-02-20

文章信息/Info

Title:
Diversity of bacterial community in rhizosphere soil of healthy and root susceptible tobacco plants in continuous cropping tobacco fields
作者:
敖金成1 李永梅1 李博2
1.云南农业大学植物保护学院,云南昆明 650201; 2.云南农业大学资源与环境学院,云南昆明 650201
Author(s):
Ao Jinchenget al
关键词:
高通量测序连作烟田细菌群落冗余分析根际土壤多样性分析
Keywords:
-
分类号:
S154.36;S154.37
DOI:
-
文献标志码:
A
摘要:
为探明连作与感染根腐病烟株根际土壤细菌群落结构变化,采用Illumina高通量技术对细菌16S rDNA的V3-V4区域进行测序,对比研究连作烟田健株与感染根腐病烟株根际土壤细菌群落结构。结果表明,变形菌门、放线菌门、浮霉菌门、酸杆菌门、芽单胞菌门、绿弯菌门是连作烟田健株和病株根际土壤主要的细菌优势种群,相对丰度累计总和为89.1%~93.9%,随连作年限的延长各优势种群发生趋向性变化;随连作年限的延长,健株根际土壤细菌群落丰富度和多样性呈先增后降趋势,病株根际土壤细菌群落丰富度和多样性呈降低趋势,短期连作(2~4 年)烟田健株和病株根际土壤细菌群落组成较为相似,与连作8年和撂荒2年以上(CK)土壤样本的细菌群落组成差异较大;冗余分析结果表明,土壤化学因子与细菌群落分布关联紧密,其中pH值是影响土壤细菌群落分布的核心因子,其贡献度为15.77%。综上,连作和染病降低了土壤细菌群落丰富度和多样性,优势菌群趋向性演化,有益菌群相对丰度降低。在生产实践中,提高烟田土壤pH值,降低连作年限并及时清除烟株病残体有利于维持土壤微生态环境的稳定性。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2021-05-26
基金项目:国家自然科学基金(编号:32060445、41807524);云南省自然科学基金(编号:202001AU070114);广西中烟工业有限责任公司科技项目(编号:GXZYCX2019b004)。
作者简介:敖金成(1984—),男,云南曲靖人,博士研究生,高级工程师,主要从事植物营养与病害控制技术研究。E-mail:89693180@qq.com。
通信作者:李永梅,博士,教授,博士生导师,主要从事植物营养与病害控制技术研究。E-mail:youngmaylee@126.com。
更新日期/Last Update: 2022-02-20