|本期目录/Table of Contents|

[1]尹义旭,汤伟,咸洪泉.重组毕赤酵母表达棘孢木霉几丁质酶gene02524的酶学性质及抑菌活性[J].江苏农业科学,2022,50(9):96-103.
　Yin Yixu,et al.Enzymatic properties and antibacterial activity of recombinant Pichia pastoris expressing chitinase gene02524 from Trichoderma asperellum[J].Jiangsu Agricultural Sciences,2022,50(9):96-103.
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重组毕赤酵母表达棘孢木霉几丁质酶gene02524的酶学性质及抑菌活性(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第9期
页码:
96-103
栏目:
植物保护
出版日期:
2022-05-05

文章信息/Info

Title:
Enzymatic properties and antibacterial activity of recombinant Pichia pastoris expressing chitinase gene02524 from Trichoderma asperellum
作者:
尹义旭1; 汤伟1; 咸洪泉1; 2
1.青岛农业大学生命科学学院，山东青岛 266109； 2.山东省应用真菌重点实验室，山东青岛 266109
Author(s):
Yin Yixu; et al
关键词:
棘孢木霉; 几丁质酶; 基因表达; 毕赤酵母; 酶学性质
Keywords:
-
分类号:
S188+.3
DOI:
-
文献标志码:
A
摘要:
几丁质酶是催化降解几丁质的水解酶，在防治植物真菌病害与虫害方面的应用越来越广泛，是一种重要的生防蛋白。棘孢木霉TD3104是一株优良的植物病害生防菌，几丁质酶在抑菌防病过程中发挥重要作用。为克隆表达棘孢木霉TD3104几丁质酶基因gene02514，明确酶学性质和抑菌活性，利用毕赤酵母表达系统对目的蛋白进行表达，并通过SephadexG-100凝胶进行纯化，对序列进行分析并研究其酶学性质及进行体外抑菌试验。本研究成功地构建了pPIC9K/gene02524重组载体，并且经过比对发现其包含GH18家族的特征氨基酸序列，获得了毕赤酵母转几丁质酶基因工程菌，表达的重组几丁质酶表观分子量44.4 ku，Km=2.048 2 g/L，Vmax=0.635 5×103 μmol/(L·min)，最适反应温度为50 ℃，最适pH值为5.0，在pH值为3.5时稳定性最高；金属离子Cu2+、Hg2+可强烈抑制酶活性；可抑制金黄壳囊孢菌等病原真菌的生长。研究结果为解析棘孢木霉几丁质酶在生防中的作用及功能奠定了基础，并为植物病虫害的防治提供了新的基因资源。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2022-02-28
基金项目：山东省重点研发计划(编号：2018GNC110024)；山东省青岛市科技惠民示范引导专项(编号：21-1-4-ny-5-nsh)。
作者简介：尹义旭(1996—)，男，山东泰安人，硕士研究生，研究方向为生物工程，E-mail：yyx960930@163.com；共同第一作者：汤伟(1986—)，女，山东青岛人，博士，研究方向为微生物学，E-mail：tianti_121@ 163. com。
通信作者：咸洪泉，博士，教授，研究方向为应
更新日期/Last Update: 2022-05-05