|本期目录/Table of Contents|

[1]吉颖,吴淑华,崔晓艳,等.江苏省大豆上一种新粉虱传病毒的分子鉴定及系统进化分析[J].江苏农业科学,2022,50(10):30-36.
　Ji Ying,et al.Molecular identification and phylogenetic analysis of a new whitefly-transmitted virus infecting soybean from Jiangsu Province[J].Jiangsu Agricultural Sciences,2022,50(10):30-36.
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江苏省大豆上一种新粉虱传病毒的分子鉴定及系统进化分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第10期
页码:
30-36
栏目:
生物技术
出版日期:
2022-05-20

文章信息/Info

Title:
Molecular identification and phylogenetic analysis of a new whitefly-transmitted virus infecting soybean from Jiangsu Province
作者:
吉颖1; 吴淑华2; 崔晓艳3; 涂丽琴2; 高丹娜2; 程兆榜2; 周益军2; 陈新3; 季英华2; 郭青云1
1.青海大学农林科学院/青海省农业有害生物综合治理重点实验室/农业农村部西宁作物有害生物科学观测实验站，青海西宁 810016；2.江苏省农业科学院植物保护研究所，江苏南京 210014； 3.江苏省农业科学院经济作物研究所，江苏南京 210014
Author(s):
Ji Ying; et al
关键词:
大豆; 豇豆轻斑驳病毒; 分子鉴定; 聚类分析; 进化树构建
Keywords:
-
分类号:
S435.651
DOI:
-
文献标志码:
A
摘要:
2019年在对江苏大豆上病毒病进行调查时，发现徐州和淮安大豆田间出现一种表现叶片黄化、花叶症状的病毒病，为明确其伴随病毒种类，对田间采集的70份疑似病样进行分子检测和鉴定，结果在利用香石竹潜隐病毒属(Carlavirus)通用引物检测时发现，70份疑似病样中有15份样品扩增到800 bp左右的目的片段，序列测定后BLAST分析结果显示，其与一种粉虱传病毒——豇豆轻斑驳病毒CpMMV(cowpea mild mottle virus，CpMMV)的同源性最高，暗示了其可能是CpMMV的一个分离物。针对CpMMV编码的CP基因设计特异性引物，检测结果显示15份阳性样品均扩增到目的片段，对获得的CP基因片段进行克隆测序，发现其序列全长867 bp，编码分子量约为32ku的蛋白；序列聚类分析结果显示，其与已报道的CpMMV分离物有较高的同源性，并聚类到同一个大的分支，其中与安徽、海南等地分离物相对近缘，聚在同一个小分支。这些结果表明江苏大豆存在豇豆轻斑驳病毒的侵染危害，生产上需要密切关注。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2022-01-14
基金项目：国家自然科学基金(编号：32072506)；现代农业产业技术体系建设专项(编号：CARS-24-C-01)；江苏省农业自主创新资金［编号：CX(21)1011］；青海省农业有害生物综合治理重点实验室项目(编号：2021-ZJ-Y08)。
作者简介：吉颖(1997—)，男，江西赣州人，硕士研究生，研究方向为园艺蔬菜作物病毒。E-mail：1298723368@qq.com。
通信作者：季英华，研究员，主要从事蔬菜病毒病成灾机理及防控技术研究，E-mail：jiyinghua@jaas.ac.cn；郭青云，研究员，主要从事植物病害生物防治研究，E-mail：guoqingyunqh@163.com。
更新日期/Last Update: 2022-05-20