|本期目录/Table of Contents|

[1]楚宗丽,李亮杰,姬虹,等.小麦Hsp70基因家族鉴定及蛋白互作网络分析[J].江苏农业科学,2022,50(10):37-44.
 Chu Zongli,et al.Identification and protein interaction network analysis of wheat Hsp70 gene family[J].Jiangsu Agricultural Sciences,2022,50(10):37-44.
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小麦Hsp70基因家族鉴定及蛋白互作网络分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第10期
页码:
37-44
栏目:
生物技术
出版日期:
2022-05-20

文章信息/Info

Title:
Identification and protein interaction network analysis of wheat Hsp70 gene family
作者:
楚宗丽李亮杰姬虹程琴孙君艳
信阳农林学院,河南信阳 464006
Author(s):
Chu Zongliet al
关键词:
小麦Hsp70热激蛋白基因鉴定蛋白互作
Keywords:
-
分类号:
S512.101
DOI:
-
文献标志码:
A
摘要:
为研究小麦Hsp70蛋白的相互作用,利用生物信息学方法,在全基因组范围内对小麦Hsp70基因家族进行鉴定,并进行蛋白互作及可视化分析。结果表明,在全基因组范围内共鉴定到21个结构域高度保守的Hsp70基因家族成员,亚细胞定位显示主要分布于细胞质,蛋白的二级结构中,α-螺旋与无规卷曲类型在每个蛋白中所占比重较大,二者之和大于70%,延伸链和β-转角所占比重较小,二者之和小于30%。互作分析结果表明,TaHsp70-14、TaHsp70-6、TaHsp70-12、TaHsp70-16、TaHsp70-3,TaHsp70-8、TaHsp70-21、TaHsp70-20,TaHsp70-17和TaHsp70-11之间有相互作用,GO分析显示所鉴定基因主要在分子功能、生物过程及细胞成分方面起作用。
Abstract:
-

参考文献/References:

[1]Mishra D,Shekhar S,Agrawal L,et al. Cultivar-specific high temperature stress responses in bread wheat (Triticum aestivum L.) associated with physicochemical traits and defense pathways[J]. Food Chemistry,2017,221:1077-1087.
[2]Nakajima Y,Suzuki S. Environmental stresses induce misfolded protein aggregation in plant cells in a microtubule-dependent manner[J]. International Journal of Molecular Sciences,2013,14(4):7771-7783.
[3]Wang W X,Vinocur B,Altman A. Plant responses to drought,salinity and extreme temperatures:towards genetic engineering for stress tolerance[J]. Planta,2003,218(1):1-14.
[4]Xue G P,Drenth J,McIntyre C L. TaHsfA6f is a transcriptional activator that regulates a suite of heat stress protection genes in wheat (Triticum aestivum L.) including previously unknown Hsf targets[J]. Journal of Experimental Botany,2014,66(3):1025-1039.
[5]Wang W X,Vinocur B,Shoseyov O,et al. Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response[J]. Trends in Plant Science,2004,9(5):244-252.
[6]Morimoto R I. Regulation of the heat shock transcriptional response:cross talk between a family of heat shock factors,molecular chaperones,and negative regulators[J]. Genes & Development,1998,12(24):3788-3796.
[7]Feder M E. Organismal,ecological,and evolutionary aspects of heat-shock proteins and the stress response:established conclusions and unresolved issues[J]. Integrative and Comparative Biology,2015,39(6):857-864.
[8]王明强,张道远. 植物热激蛋白70基因家族及其生物学功能研究进展[J]. 基因组学与应用生物学,2015,34(2):421-428.
[9]Park C J,Seo Y S. Heat shock proteins:a review of the molecular chaperones for plant immunity[J]. The Plant Pathology Journal,2015,31(4):323-333.
[10]Lin B L,Wang J S,Liu H C,et al. Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana[J]. Cell Stress & Chaperones,2001,6(3):201-208.
[11]Mishra R C,Grover A. ClpB/Hsp100 proteins and heat stress tolerance in plants[J]. Critical Reviews in Biotechnology,2016,36(5):862-874.
[12]Li J,Liu X H. Genome-wide identification and expression profile analysis of the Hsp20 gene family in barley (Hordeum vulgare L.)[J]. PeerJ,2019,7:e6832.
[13]Siddique M,Gernhard S,von Koskull-Dring P,et al. The plant sHSP superfamily:five new members in Arabidopsis thaliana with unexpected properties[J]. Cell Stress & Chaperones,2008,13(2):183-197.
[14]Corigliano M G,Maglioco A,Laguía Becher M,et al. Plant Hsp90 proteins interact with B-cells and stimulate their proliferation[J]. PLoS One,2011,6(6):e21231.
[15]杜巧丽,蒋君梅,陈美晴,等. 水稻热休克蛋白Hsp70基因克隆、表达分析及原核表达[J]. 植物保护学报,2021,48(3):620-629.
[16]Duan Y H,Guo J,Ding K,et al. Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses[J]. Molecular Biology Reports,2011,38(1):301-307.
[17]刘阳. 普通小麦热激蛋白70基因家族分析及TaHsp70-47的克隆[D]. 杨凌:西北农林科技大学,2020.
[18]安艳秋,蔺瑞明,冯晶,等. 小麦热激蛋白基因TaHSP70克隆及其在植物防卫和抗逆反应中的表达分析[J]. 分子植物育种,2011,9(4):402-409.
[19]Jung K H,Gho H J,Nguyen M X,et al. Genome-wide expression analysis of HSP70 family genes in rice and identification of a cytosolic HSP70 gene highly induced under heat stress[J]. Functional & Integrative Genomics,2013,13(3):391-402.
[20]宋晋辉,马海莲,瓮巧云,等. 玉米Hsp70基因家族的全基因组鉴定与分析[J]. 核农学报,2017,31(7):1245-1254.
[21]Augustine S M,Cherian A V,Syamaladevi D P,et al. Erianthus arundinaceus HSP70 (EaHSP70) acts as a key regulator in the formation of anisotropic interdigitation in sugarcane (Saccharum spp. hybrid) in response to drought stress[J]. Plant and Cell Physiology,2015,56(12):2368-2380.
[22]郭猛. 辣椒热胁迫相关基因表达分析及功能研究[D]. 杨凌:西北农林科技大学,2016.
[23]赵溪,张婷婷,邢文婷,等. 铁皮石斛热激蛋白HSP70家族基因鉴定及温度胁迫下的表达分析[J]. 园艺学报,2021,48(9):1743-1754.
[24]Rowarth N M,Dauphinee A N,Denbigh G L,et al. Hsp70 plays a role in programmed cell death during the remodelling of leaves of the lace plant (Aponogeton madagascariensis)[J]. Journal of Experimental Botany,2020,71(3):907-918.
[25]Latijnhouwers M,Xu X M,Mller S G. Arabidopsis stromal 70-kDa heat shock proteins are essential for chloroplast development[J]. Planta,2010,232(3):567-578.
[26]Jiang C H,Bi Y K,Zhang R Y,et al. Expression of RcHSP70,heat shock protein 70 gene from Chinese rose,enhances host resistance to abiotic stresses[J]. Scientific Reports,2020,10:2445.
[27]Chaudhary R,Baranwal V K,Kumar R,et al. Genome-wide identification and expression analysis of Hsp70,Hsp90,and Hsp100 heat shock protein genes in barley under stress conditions and reproductive development[J]. Functional & Integrative Genomics,2019,19(6):1007-1022.
[28]王鹏飞,苏玲,蒋锡龙,等. 全基因组范围葡萄热休克转录因子的鉴定与分析[J]. 安徽农业科学,2017,45(32):144-151,227.
[29]Song Z P,Pan F L,Lou X P,et al. Genome-wide identification and characterization of Hsp70 gene family in Nicotiana tabacum[J]. Molecular Biology Reports,2019,46(2):1941-1954.
[30]Jacob P,Hirt H,Bendahmane A.The heat-shock protein/chaperone network and multiple stress resistance[J]. Plant Biotechnology Journal,2017,15(4):405-414.

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备注/Memo

备注/Memo:
收稿日期:2021-09-30
基金项目:信阳农林学院高水平科研孵化器建设项目(编号:FCL202007);河南省科技攻关(编号:212102110049);信阳农林学院校青年基金(编号:20200104)。
作者简介:楚宗丽(1979— ),女,河南商丘人,博士,讲师,主要从事小麦遗传育种研究。E-mail: chuzl10000@126.com。
通信作者:孙君艳,硕士,教授,主要从事小麦栽培技术研究。E-mail:xysjy66@163.com。
更新日期/Last Update: 2022-05-20